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  4. Recombinant Mouse Phosphatidylinositol 3-kinase regulatory subunit alpha (Pik3r1)

Recombinant Mouse Phosphatidylinositol 3-kinase regulatory subunit alpha (Pik3r1)

  • 中文名稱：
    小鼠Pik3r1重組蛋白
  • 貨號：
    CSB-EP018003MO
  • 規(guī)格：
    ￥1536
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
  • 生物活性：
    Not Test
  • 基因名：
  • Uniprot No.：
  • 別名：
    PI3-kinase regulatory subunit alpha;PI3K regulatory subunit alpha;PtdIns-3-kinase regulatory subunit alpha;Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha;PI3-kinase subunit p85-alpha;PtdIns-3-kinase regulatory subunit p85-alpha
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Full Length of Mature Protein
  • 來源：
    E.coli
  • 分子量：
    90.3 kDa
  • 表達區(qū)域：
    2-724aa
  • 氨基酸序列
    SAEGYQYRALYDYKKEREEDIDLHLGDILTVNKGSLVALGFSDGQEARPEDIGWLNGYNETTGERGDFPGTYVEYIGRKRISPPTPKPRPPRPLPVAPGSSKTEADTEQQALPLPDLAEQFAPPDVAPPLLIKLLEAIEKKGLECSTLYRTQSSSNPAELRQLLDCDAASVDLEMIDVHVLADAFKRYLADLPNPVIPVAVYNEMMSLAQELQSPEDCIQLLKKLIRLPNIPHQCWLTLQYLLKHFFKLSQASSKNLLNARVLSEIFSPVLFRFPAASSDNTEHLIKAIEILISTEWNERQPAPALPPKPPKPTTVANNSMNNNMSLQDAEWYWGDISREEVNEKLRDTADGTFLVRDASTKMHGDYTLTLRKGGNNKLIKIFHRDGKYGFSDPLTFNSVVELINHYRNESLAQYNPKLDVKLLYPVSKYQQDQVVKEDNIEAVGKKLHEYNTQFQEKSREYDRLYEEYTRTSQEIQMKRTAIEAFNETIKIFEEQCQTQERYSKEYIEKFKREGNEKEIQRIMHNHDKLKSRISEIIDSRRRLEEDLKKQAAEYREIDKRMNSIKPDLIQLRKTRDQYLMWLTQKGVRQKKLNEWLGNENTEDQYSLVEDDEDLPHHDEKTWNVGSSNRNKAENLLRGKRDGTFLVRESSKQGCYACSVVVDGEVKHCVINKTATGYGFAEPYNLYSSLKELVLHYQHTSLVQHNDSLNVTLAYPVYAQQRR
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標簽：
    C-terminal 6xHis-tagged
  • 產品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • Datasheet & COA：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling. Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement.
  • 基因功能參考文獻：
    1. the P85 subunit plays an essential role in neuronal migration and neuronal morphology during mouse corticogenesis. PMID: 29130119
    2. A RAB35-p85/PI3K axis controls oscillatory apical protrusions required for efficient chemotactic migration. PMID: 29662076
    3. Alk1 deletion leads to increased endothelial PI3K pathway activation that may be a novel target for the treatment of vascular lesions in hereditary haemorrhagic telangiectasia type 2. PMID: 27897192
    4. A central role of PI3K in development of obesity and fatty liver disease, separating these effects from the role of PI3K in insulin resistance and the resultant hyperglycemia. PMID: 29724723
    5. Findings provide evidence that Pik3r1 downregulation promotes hyperglycaemia probably by impairing GLUT4-facilitated glucose uptake. PMID: 27199286
    6. TLR2 confers a pivotal role in allergic airway inflammation via regulating the PI3K/Akt signaling pathway-related autophagy in mice. PMID: 28493079
    7. These data suggest that the antidepressant-like effects of VPA might depend on PI3K and mTOR activation. PMID: 28408298
    8. These findings highlight a hitherto unexplored and novel role for Cbl and PI3K in modulating the osteogenic response of periosteal cells during the early stages of fracture repair. PMID: 27884787
    9. Erk5 MAP kinase is activated in response to PDGF-BB in the smooth muscle cell line MOVAS in a manner dependent on Mekk2, Mek1/2, Mek5, PI3-kinase and protein kinase C (PKC). PMID: 27339033
    10. Data demonstrated a novel role of Pik3r1 that was independent of the regulatory function of phosphoinositide 3-kinase in mediating the metabolic action of glucocorticoids. Glucocorticoids increased hormone sensitive lipase phosphorylation, but not Plin1 phosphorylation, in adipose tissue-specific Pik3r1-null (AKO) mice. PMID: 28292967
    11. Collectively, these findings showed that SA suppressed mammary gland development of pubertal mice, which was coincident with the SA-inhibited HC11 proliferation, and was associated with inhibition of PI3K/Akt signaling pathway through activation of GPR120. PMID: 28712865
    12. suggest that small-bowel resection reduces p85alpha and TP53, which increases survivin and intestinal epithelial cell expansion during therapeutic adaptation in patients with short bowel syndrome PMID: 27157990
    13. These results indicate that there is a tissue-specific regulation of IGF-1/PI3K/Akt during normal aging and upon dietary restriction to help maintain the metabolic status of these tissues at those phases of animal's lifespan. PMID: 26372898
    14. Uncarboxylated osteocalcin inhibits high glucose-induced reactive oxygen species production and stimulates osteoblastic differentiation by inhibiting the activation of PI3K/Akt in MC3T3-E1 cells. PMID: 26719856
    15. miR-130a plays a critical role in attenuation of cardiac dysfunction and remodeling after myocardial infarction. The mechanisms involve activation of PI3K/Akt signaling via suppression of PTEN expression. PMID: 26458524
    16. PI3K/AKT pathway was partially involved in the modulation of the release HMGB-1 from reactive astrocytes. PMID: 26115623
    17. These data demonstrate the ability of this heterozygous mutation to alter PI3K activity in vivo and the central role of PI3K in insulin/growth factor action, adipocyte function, and glucose metabolism. PMID: 26974159
    18. These results suggested that cordycein is a highly selective treatment to induce MA-10 cells apoptosis via p38 MAPKs signaling. PMID: 26303320
    19. A Cytosolic Multiprotein Complex Containing p85alpha Is Required for beta-Catenin Activation in Colitis and Colitis-associated Cancer. PMID: 26565021
    20. Our results thus demonstrated that GalT deficiency can effect down-regulation of the PI3K/Akt growth signaling pathway in mouse fibroblasts through distinct mechanisms targeting both gene and protein expression levels. PMID: 26773505
    21. Our results indicate HDM extract induced the expression of VEGF, TGF-beta1, and FGF-2 by activating the PI3K/Akt/HIF-1alpha pathway in human primary cultured NECs and in the nasal mucosa of a murine model. PMID: 26740176
    22. Apelin regulates inflammatory response, diminishes inflammatory remote organ damage and improves survival in an experimental model of burn sepsis, which is at least partly mediated by a phosphatidylinositol 3-kinase/protein kinase B dependent pathway. PMID: 26163884
    23. Y276 phosphorylation is necessary for enhanced CD300f-mediated phagocytosis through the recruitment of the p85alpha regulatory subunit of phosphatidylinositol-3-kinase PMID: 24477292
    24. Defective podocyte insulin signaling through p85-XBP1 promotes ATF6-dependent maladaptive ER-stress response in diabetic nephropathy. PMID: 25754093
    25. PI3K and p38 play an important role in the suppression of differentiation of erythroid precursors after blood loss. PMID: 25711660
    26. these results suggest that PPARgamma is essential for the transcriptional activity of PIK3R1 during adipogenesis PMID: 24859974
    27. Insulin-phosphatidylinositol 3-kinase-Forkhead box class O3 (FOXO3) signaling is required for circadian rhythmicity in the liver via regulation of Clock. FoxO3 knockdown dampens circadian amplitude, an effect that is rescued by overexpression of Clock. PMID: 24856209
    28. starvation activates the autophagic PI3K, which in turn induces the recruitment of COPII to the endoplasmic reticulum-Golgi intermediate compartment to bud LC3 lipidation-active vesicles as one potential membrane source of the autophagosome. PMID: 25432021
    29. Our observations also point towards PI3K acting as a morphogenic switch for FGF7 signalling PMID: 25460003
    30. The PTEN/PI3K/AKT signaling axis controls hypoxia-induced HIF1alpha (HIF1A) and HIF2alpha (EPAS1) stability in macrophages. PMID: 25103499
    31. The identification of PI3K pathway deregulation as both an early event in the neoplastic transformation of the gallbladder epithelium and a main mechanism of tumor growth in Pten heterozygous. PMID: 24658595
    32. Its signaling is involved in neuroprotection. PMID: 25004888
    33. blockade of p85-PTEN interaction and PTEN inhibition may be promising strategies for rescuing the heart from ischemia and reperfusion injury. PMID: 24752319
    34. Whereas Cbl-PI3K interaction regulates differentiation and survival, bone resorption is predominantly regulated by Cbl-b in osteoclasts. PMID: 24470255
    35. TLR4 and PI3K effects on BST-2 induction are at the level of transcription. PMID: 24036213
    36. LPS-induced activation of phosphoinositide 3-kinase (PI3K) in adipocytes. PMID: 24506665
    37. The downstream signals and mechanism of the PI3K signal pathway mediate apoptosis in neural progenitor cells. PMID: 24022164
    38. Although it also depended on JAK/STAT- and PI3K signalling. PMID: 24825007
    39. TRbeta acts as a cytoplasmic, phosphotyrosine-dependent scaffold for the p85 regulatory subunit of PI3K and the Src kinase Lyn in the absence of thyroid hormone. PMID: 24932806
    40. Treatment of LPS treated IL-14+PTEN-/- mice with a pan PI-3K inhibitor. PMID: 24881819
    41. These results suggest that PI3K/Akt signals play a role in the GDF5-mediated brown adipogenesis through a mechanism related to activation of the Smad pathway. PMID: 24944017
    42. The results of this study indicated that PI3Kgamma contributes to development of autoimmune CNS inflammation in mice/ PMID: 24012746
    43. This study presentes that CaN is critical for neural induction during embryonic development and ESC differentiation. PMID: 24698271
    44. BRD7-mediated translocation of a subfraction (but not all) of the p85 protein to the nucleus could enhance PI3K signaling. PMID: 24657164
    45. Activation of the MEK/ERK, PI3K/Akt, and inhibition of Wnt/beta-catenin signaling pathways is involved in the melanogenesis. PMID: 24129178
    46. Lower activity of the PI3K/Akt-axis. PMID: 23874604
    47. By activating the TCF1/beta-catenin axis via the PI3K/AKT/ERK pathway. PMID: 23874982
    48. modulation of p85alpha can protect pancreatic beta-cells from endoplasmic reticulum stress-induced apoptosis PMID: 24395790
    49. cell-type-specific miRNA dysregulation and subsequent PI3K/Akt activation may be involved in the transition from intestinal inflammation to cancer PMID: 24464560
    50. GLP-2 plays a key physiological role in the control of hepatic glucose production through activating PI3K-dependent modulation of membrane excitability and nuclear transcription of POMC neurons in the brain. PMID: 23823479

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  • 蛋白家族：
    PI3K p85 subunit family
  • 數(shù)據(jù)庫鏈接：