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  4. Recombinant Mouse Telomere-associated protein RIF1 (Rif1), partial

Recombinant Mouse Telomere-associated protein RIF1 (Rif1), partial

  • 中文名稱：
    小鼠Rif1重組蛋白
  • 貨號：
    CSB-YP744143MO
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Rif1重組蛋白
  • 貨號：
    CSB-EP744143MO
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Rif1重組蛋白
  • 貨號：
    CSB-EP744143MO-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Rif1重組蛋白
  • 貨號：
    CSB-BP744143MO
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Rif1重組蛋白
  • 貨號：
    CSB-MP744143MO
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    Rif1; Telomere-associated protein RIF1; Rap1-interacting factor 1 homolog; mRif1
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Key regulator of TP53BP1 that plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage: acts by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs. In response to DNA damage, interacts with ATM-phosphorylated TP53BP1. Interaction with TP53BP1 leads to dissociate the interaction between NUDT16L1/TIRR and TP53BP1, thereby unmasking the tandem Tudor-like domain of TP53BP1 and allowing recruitment to DNA DSBs. Once recruited to DSBs, RIF1 and TP53BP1 act by promoting NHEJ-mediated repair of DSBs. In the same time, RIF1 and TP53BP1 specifically counteract the function of BRCA1 by blocking DSBs resection via homologous recombination (HR) during G1 phase. Also required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs. Promotes NHEJ of dysfunctional telomeres.
  • 基因功能參考文獻(xiàn)：
    1. Rif1 is a high-affinity binding partner of protein phosphatase 1. PMID: 28522851
    2. Knockdown of RIF1 increases cisplatin sensitivity in vivo in Epithelial Ovarian Cancer. PMID: 29719287
    3. Findings indicate an essential role of telomere-associated protein Rif1 in the epigenetic defense against endogenous retroviruses (ERVs) activation. PMID: 29040764
    4. This study identifies Rif1 as the molecular link between nuclear architecture and replication-timing establishment in mammals. PMID: 26725008
    5. Oct4 recruits Smad3 to bind to Rif1 promoter, but Smad3 joining facilitates the loading of a polycomb complex that generates a repressive epigenetic modification on Rif1 promoter and maintains the expression of Rif1 in embryonic stem cells. PMID: 25569105
    6. structural study of the mammalian Rif1 PMID: 24634216
    7. Rif1 negatively modulates Zscan4 expression by maintaining H3K9me3 levels at subtelomeric regions. Rif1 interacts and stabilizes H3K9 methylation complex. PMID: 24735877
    8. Rif1(-/-) mice are severely compromised for 53BP1-dependent class switch recombination (CSR) and fusion of dysfunctional telomeres. PMID: 23333305
    9. study identifies Rif1 as an ATM phosphorylation-dependent interactor of 53BP1 and shows that absence of Rif1 results in 5'-3' DNA-end resection; Rif1 deficiency impairs DNA repair in cell cycle G(1) and S phases, interferes with immunoglobulin class switchnig and leads to accumulation of chromosome double strand breaks PMID: 23306439
    10. Rif1 is a key regulator of the replication-timing programme in mammalian cells PMID: 22850673
    11. Rif1 has a role in the repair of stalled replication forks by facilitating homology-directed repair. PMID: 19948482
    12. This is a mouse ortholog of the yeast Rif1 family of telomere-associated proteins identified on the basis of its high expression in primordial germ cells and embryo-derived pluripotent stem cell lines. PMID: 15042697

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  • 亞細(xì)胞定位：
    Nucleus. Chromosome. Chromosome, telomere. Cytoplasm, cytoskeleton, spindle.
  • 蛋白家族：
    RIF1 family
  • 組織特異性：
    Expressed in Sertoli cells, prospermatagonia, early primary spermatocytes, and in oocytes at all stages of their growth. Expressed in embryonic stem (ES) and embryonic germ (EG) cells: expression is lost upon differentiation.
  • 數(shù)據(jù)庫鏈接：