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  4. Recombinant Mouse Tyrosine-protein kinase JAK3 (Jak3), partial

Recombinant Mouse Tyrosine-protein kinase JAK3 (Jak3), partial

  • 中文名稱：
    小鼠Jak3重組蛋白
  • 貨號：
    CSB-EP717252MO
  • 規(guī)格：
    ￥1836
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 別名：
    Jak3; Tyrosine-protein kinase JAK3; EC 2.7.10.2; Janus kinase 3; JAK-3
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Partial
  • 來源：
    E.coli
  • 分子量：
    36.2kDa
  • 表達區(qū)域：
    818-1100aa
  • 氨基酸序列
    LKYISLLGKGNFGSVELCRYDPLGDNTGPLVAVKQLQHSGPDQQRDFQREIQILKALHSDFIVKYRGVSYGPGRQSLRLVMEYLPSGCLRDFLQRHRARLHTDRLLLFAWQICKGMEYLGARRCVHRDLAARNILVESEAHVKIADFGLAKLLPLGKDYYVVREPGQSPIFWYAPESLSDNIFSRQSDVWSFGVVLYELFTYCDKSCSPSAEFLRMMGPEREGPPLCRLLELLAEGRRLPPPPTCPTEVQELMQLCWAPSPHDRPAFGTLSPQLDALWRGRPG
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標簽：
    N-terminal 6xHis-tagged
  • 產品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation. Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A AND STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion.
  • 基因功能參考文獻：
    1. Study investigated the effect of Jak3 signaling on differentiation from nestin progenitor cells using E13.5 spinal progenitor cell cultures. Results indicated that neuronal and microglial cell differentiation was regulated primarily by Jak3 signaling and the developing neurons and neurite outgrowth might also be regulated by Jak3-dependent microglial activity. PMID: 28817455
    2. JAK3 up-regulates SGLT1 activity by increasing the carrier protein abundance in the cell membrane, an effect enforcing cellular glucose uptake into activated lymphocytes and thus contributing to the immune response. PMID: 27595398
    3. Foxp3 has a rapid turn over in Treg partly controlled at the transcriptional level by the JAK/STAT pathway PMID: 27077371
    4. JAK3 contributes to the regulation of membrane Kv1.5 protein abundance and activity, an effect sensitive to ouabain and thus possibly involving Na(+)/K(+) ATPase activity. PMID: 26100849
    5. JAK3 deficiency is followed by down-regulation of cytosolic Ca(2+) release, receptor and store operated Ca(2+) entry and Na(+)/Ca(2+) exchanger activity in dendritic cells. PMID: 26279433
    6. Jak3 has a role in promoting mucosal tolerance through suppressed expression and limiting activation of TLRs thereby preventing intestinal and systemic chronic low-grade inflammation and associated obesity and MetS PMID: 26451047
    7. Our results demonstrate that JAK3/STAT6 has an important role in bone marrow-derived fibroblast activation, extracellular matrix production, and interstitial fibrosis development. PMID: 26032813
    8. results showed that different JAK3 mutations induce constitutive activation through distinct mechanisms, pointing to specific therapeutic perspectives PMID: 26446793
    9. JAK3 down-regulates Na(+)/K(+)-ATPase activity, an effect involving gene expression and profoundly curtailing ATP consumption. PMID: 26021261
    10. In conclusion, JAK3 deficiency leads to increased formation of calcitriol, which contributes to or even accounts for increased release of FGF23 and enhanced intestinal phosphate absorption. PMID: 25493954
    11. JAK3 deficiency leads to intestinal sodium (Na+) loss, decrease of blood pressure, increased aldosterone release and subsequent stimulation of renal tubular Na(+) reabsorption. PMID: 24281140
    12. JAK3 is a powerful regulator of the peptide transporters PEPT1 and PEPT2. PMID: 23934551
    13. cPLA2, p38 mitogen-activated protein kinase (MAPK), and Janus kinase 3 (JAK3) signaling are necessary for F. tularensis-induced PGE2 production. PMID: 24343645
    14. the essential role of Jak3 in the colon where it facilitated mucosal differentiation by promoting the expression of differentiation markers and enhanced colonic barrier functions through AJ localization of beta-catenin. PMID: 24045942
    15. The potential effects of pharmacological JAK3 inhibition in a myocardial ischemia and reperfusion injury model, were investigated. PMID: 23680658
    16. Inhibition of JAK3 suppressed phosphorylation of PI3K downstream effectors including Akt, mammalian target of rapamycin complex 1, glycogen synthase kinase 3beta, and CREB. JAK3 differentially regulates TLR-mediated inflammatory cytokine production. PMID: 23797672
    17. hemagglutinin of H5N1 avian influenza triggered an abnormal innate immune signalling in the pulmonary epithelial cells, through an unusual process involving activation of Janus kinase 3 PMID: 22359619
    18. analysis of the biochemistry, immunological functions, and clinical significance of JAK3 [review] PMID: 22130498
    19. These findings highlight the pathological role of CD8(+) T cells and Jak3-dependent IFN-gamma-mediated Th1 responses in cerebral malaria pathogenesis. PMID: 22363534
    20. JAK3 inhibition potently facilitates myoblast differentiation through antagonistic STAT1/STAT3 activities. PMID: 22120524
    21. dominant-negative mutants of either Cullin1 or Cullin5, which lack the C terminus responsible for recruiting the E2 enzymes, were able to prevent JAK3 degradation induced by both ASB2/SKP2 and NOTCH signaling. PMID: 21969365
    22. This study defines a novel JAK3 mutation among patients with N-DS AML and demonstrates that normal individuals can also display germline JAK3 substitutions, previously proven to have oncogenic properties, in vitro and in vivo. PMID: 21599579
    23. Janus kinase 3 is expressed in erythrocytes, phosphorylated upon energy depletion and involved in the regulation of suicidal erythrocyte death. PMID: 21691072
    24. Human cord blood-derived CD34+ hematopoietic stem cells were successfully engrafted into Balb/c Rag-2-/-Jak3-/- mice; however, the engraftment rate was far lower in C57/BL6 Rag-2-/-Jak3-/- mice. PMID: 21331358
    25. Jak3 inhibition induced neuronal differentiation accompanied by neurite growth. PMID: 20979137
    26. the CD69 cytoplasmic tail associates with the Jak3/Stat5 signaling pathway, which regulates the transcription of RORgammat and, consequently, differentiation toward the Th17 lineage PMID: 20696842
    27. despite its dependence on leukemia inhibitory factor, JAK3 is not essential for LIF-mediated photoreceptor protection or gene expression. PMID: 20345762
    28. Jak3 is important for dendritic cell maturation, migration and function, through a CCR7-mediated signalling pathway PMID: 19759904
    29. Nonhematopoietic expression of Janus kinase 3 is required for eosinophilic airway inflammation PMID: 11859141
    30. plays several important roles in B cells: during development, to enable cell division, Ig gene rearrangement and cell differentiation, and in mature cells, to promote B cell survival in the periphery PMID: 12147626
    31. Activation of this pathway is associated with granulocytic differentiation of myeloid precursor cells PMID: 12351382
    32. observations establish JAK3 as a key mediator of severe GVHD after allogeneic BMT in the context of a major-HLA disparity PMID: 12389628
    33. Jak3 is a primary response gene for interleukin-6 in macrophage differentiation, and ectopic overexpression of Jak3 accelerates monocytic differentiation of normal mouse bone marrow cells stimulated with cytokines PMID: 14976041
    34. Jak3 is not essential for dendritic cell development but unexpectedly appears to be an important negative regulator PMID: 16020505
    35. the effect of IL-15 and IL-21, which are closely related to IL-2 and share the usage of the common gamma chain and of its JAK3-associated pathway. PMID: 17938255
    36. PDGFRalpha and JAK3 have roles in Galpha(12)-mediated cell proliferation PMID: 18038259
    37. The efficacy observed with CP-690550 in collagen-induced arthritis and adjuvant arthritis suggests JAK3 inhibition may represent novel therapeutic target for treatment of rheumatoid arthritis. PMID: 18234077
    38. data indicate that Janus-kinase-3 regulates epigenetic modification and chromatin remodeling of the Ifng locus during Th1 cell differentiation PMID: 18549798
    39. somatic loss of BLNK and concomitant mutations leading to constitutive activation of Jak3/STAT5 pathway result in the generation of pre-B-cell leukemia PMID: 19047679
    40. functions of JAK3 in the development of skin immune responses and diseases such as psoriasis PMID: 19596999

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  • 亞細胞定位：
    Endomembrane system; Peripheral membrane protein. Cytoplasm.
  • 蛋白家族：
    Protein kinase superfamily, Tyr protein kinase family, JAK subfamily
  • 組織特異性：
    In contrast with the ubiquitous expression of the other JAKs, JAK3 is predominantly expressed in hematopoietic tissues.
  • 數據庫鏈接：