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  4. Recombinant Vesicular stomatitis Indiana virus RNA-directed RNA polymerase L (L), partial

Recombinant Vesicular stomatitis Indiana virus RNA-directed RNA polymerase L (L), partial

  • 中文名稱：
    Recombinant Vesicular stomatitis Indiana virus RNA-directed RNA polymerase L(L) ,partial,Yeast
  • 貨號：
    CSB-YP365969VBJ
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Vesicular stomatitis Indiana virus RNA-directed RNA polymerase L(L) ,partial,Yeast
  • 貨號：
    CSB-EP365969VBJ
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Vesicular stomatitis Indiana virus RNA-directed RNA polymerase L(L) ,partial,Yeast
  • 貨號：
    CSB-EP365969VBJ-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Vesicular stomatitis Indiana virus RNA-directed RNA polymerase L(L) ,partial,Yeast
  • 貨號：
    CSB-BP365969VBJ
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Vesicular stomatitis Indiana virus RNA-directed RNA polymerase L(L) ,partial,Yeast
  • 貨號：
    CSB-MP365969VBJ
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    L
  • Uniprot No.：
  • 別名：
    LRNA-directed RNA polymerase L; Protein L; Large structural protein; Replicase; Transcriptase) [Includes: RNA-directed RNA polymerase; EC 2.7.7.48); mRNA; guanine-N(7)-)-methyltransferase; EC 2.1.1.56); GDP polyribonucleotidyltransferase; EC 2.7.7.88); Cap-specific mRNA; nucleoside-2'-O-)-methyltransferase 2; EC 2.1.1.296)]
  • 種屬：
    Vesicular stomatitis Indiana virus (strain San Juan) (VSIV)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Responsible for RNA synthesis (replicase and transcriptase), cap addition, and cap methylation. Performs also the polyadenylation of subgenomic mRNAs by a stuttering mechanism at a slipery stop site present at the end of viral genes. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N) (Probable). The viral polymerase binds to the genomic RNA at the 3' leader promoter, thereby initiating either genome replication or mRNA transcription. In the transcription mode, the polymerase performs the sequential transcription of all mRNAs using a termination-reinitiation mechanism responding to gene start and gene end signals. Some polymerase disengage from the template at each gene junction, resulting in a decreasing abundance of transcripts from the 3' to the 5' end of the genome. The first gene is the most transcribed, and the last the least transcribed (Probable). The viral phosphoprotein helps the polymerase to engage the N-RNA template and acts as processivity factor. Polyribonucleotidyl transferase (PRNTase) adds the cap structure when the nascent RNA chain length has reached few nucleotides. Ribose 2'-O methylation of viral mRNA cap precedes and facilitates subsequent guanine-N-7 methylation, both activities being carried by the viral polymerase. In the replication mode, the polymerase replicates the whole viral genome without recognizing the gene end transcriptional signals. The ability of the polymerase to override the gene end signals as it is producing the antigenome is probably due to replicative RNA becoming encapsidated with nucleoprotein as it is synthesized.
  • 基因功能參考文獻：
    1. Here, using an improved oligo-RNA capping assay with the vesiculovirus L protein, the authors showed that the Michaelis constants for GDP and pppAACAG (vesiculovirus mRNA-start sequence) are 0.03 and 0.4 muM, respectively. PMID: 28053102
    2. Authors determined by electron cryomicroscopy the structure of the VSV L protein. The density map, at a resolution of 3.8 A, has led to an atomic model for nearly all of the 2109-residue polypeptide chain, which comprises three enzymatic domains (RNA-dependent RNA polymerase, polyribonucleotidyl transferase, and methyltransferase and two structural domains. PMID: 26144317
    3. Authors shed light on requirements for domain-domain interactions and domain contiguity in viral polymerase function. PMID: 25297996
    4. Here, the authors that vesicular stomatitis virus L initiates synthesis via a de-novo mechanism that does not require N or P, but depends on a high concentration of the first two nucleotides and specific template requirements. PMID: 22246179
    5. identified a single amino acid change, D1671V, in domain VI of the L protein, which specifically abolished viral mRNA cap methylation and was responsible for both the hr and temperature-sensitive (ts) phenotypes of mutant hr1 PMID: 15919887
    6. Our results show that the VSV RdRp can access initiation signals up or downstream from a termination signal but it does so with decreasing efficiency as the intergenic regions increases. PMID: 18241907
    7. The paper reports that the L protein produces an unusual cap structure, guanosine(5')tetraphospho(5')adenosine (GppppA) PMID: 18495767
    8. This work reveals that inhibiting cap addition and cap methylation by L protein have opposing effects on polyadenylation during VSV mRNA synthesis and provides evidence in support of a link between correct 5' cap formation and 3' polyadenylation. PMID: 19073725
    9. These findings support the notion that a flexible "hinge" region separates the cap methylase domain of L proteins from upstream functions. PMID: 19793815

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  • 亞細胞定位：
    Virion. Host cytoplasm.
  • 蛋白家族：
    Rhabdoviridae protein L family
  • 數(shù)據(jù)庫鏈接：

    KEGG: vg:1489835