Your Good Partner in Biology Research

  1. 首頁
  2. 蛋白
  3. 跨膜蛋白
  4. Recombinant Human Potassium voltage-gated channel subfamily E member 1 (KCNE1)

Recombinant Human Potassium voltage-gated channel subfamily E member 1 (KCNE1)

  • 中文名稱：
    人KCNE1重組蛋白
  • 貨號(hào)：
    CSB-CF012025HU
  • 規(guī)格：
  • 來源：
    in vitro E.coli expression system
  • 其他：

產(chǎn)品詳情

  • 基因名：
    KCNE1
  • Uniprot No.：
  • 別名：
    KCNE1; Potassium voltage-gated channel subfamily E member 1; Delayed rectifier potassium channel subunit IsK; IKs producing slow voltage-gated potassium channel subunit beta Mink; Minimal potassium channel
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長(zhǎng)度：
    full length protein
  • 表達(dá)區(qū)域：
    1-129
  • 氨基酸序列
    MILSNTTAVTPFLTKLWQETVQQGGNMSGLARRSPRSSDGKLEALYVLMVLGFFGFFTLGIMLSYIRSKKLEHSNDPFNVYIESDAWQEKDKAYVQARVLESYRSCYVVENHLAIEQPNTHLPETKPSP
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標(biāo)簽：
    N-terminal 10xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Ancillary protein that assembles as a beta subunit with a voltage-gated potassium channel complex of pore-forming alpha subunits. Modulates the gating kinetics and enhances stability of the channel complex. Assembled with KCNB1 modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1. Assembled with KCNQ1/KVLQT1 is proposed to form the slowly activating delayed rectifier cardiac potassium (IKs) channel. The outward current reaches its steady state only after 50 seconds. Assembled with KCNH2/HERG may modulate the rapidly activating component of the delayed rectifying potassium current in heart (IKr).
  • 基因功能參考文獻(xiàn)：
    1. S38G mutation induced a loss-of-function of IKs due to decreasing of KCNE1 protein expression and defecting in KCNE1 protein membrane trafficking PMID: 29395134
    2. All the protein systems generated through these processes were refined by long Molecular Dynamics simulations. The refined models were analyzed extensively to infer data about the interaction of KCNQ1 channel with its accessory KCNE1 beta subunits. PMID: 28992529
    3. In lesion samples collected from children with cardiac insufficiency, for Mink-S27, we detected significant difference in AA, CC genotype frequency and allele frequency between the observation group and the control group PMID: 28829496
    4. SUMOylation of KCNQ1 is KCNE1 dependent and determines the native attributes of cardiac IKs in vivo. PMID: 28743749
    5. Equine KV7.1/KCNE1 expressed in CHO-K1 cells exhibited electrophysiological properties that are overall similar to the human orthologs; however, a slower deactivation was found which could result in more open channels at fast rates. PMID: 28917093
    6. The KCNE1 (rs1805127) appears to an independent risk factor for AF in the Uygur population. And the KCNE4 (rs12621643) was an independent risk factor for AF among both Uygurs and Hans. PMID: 28981946
    7. Western blotting analysis combined with these pharmacological data suggest that long-term insulin treatment augments KCNQ1/KCNE1 currents by increasing KCNE1 protein expression. PMID: 28882596
    8. KCNQ1/KCNE1 channel does not require phosphatidylinositol-4,5-bisphosphate (PIP2) or phosphatidylinositol-4-phosphate for anterograde trafficking, but is heavily reliant on PIP2 for channel function once at the plasma membrane. PMID: 29020060
    9. The genetic variant rs426496 in AQP2; rs591810 in AQP3 and rs1805127, rs1805128, and rs17173510, in KCNE1 were found in patients with Meniere's disease PMID: 27509294
    10. Meta-analysis suggests that the G38S polymorphism in the KCNE1 gene can significantly increase the risk of atrial fibrillation in both Chinese and white individuals. PMID: 28640127
    11. Sphingomyelin synthase 1 positively regulates KCNQ1/KCNE1 channel density in a protein kinase D-dependent manner. PMID: 27194473
    12. Receptor Species-dependent Desensitization Controls KCNQ1/KCNE1 K+ Channels as Downstream Effectors of Gq Protein-coupled Receptors.( PMID: 27834678
    13. KCNE1 encodes a modulator of KCNQ1 and KCNH2 channels. Long QT syndrome (LQTS) patients with KCNE1(G38S) had a rate-dependent repolarization abnormality similar to patients with LQT2 and, therefore, may have a potential risk to develop lethal arrhythmias. PMID: 27255646
    14. A stoichiometry of 4:4 between the alpha KCNE1 and the beta KCNQ1-subunits has been established. PMID: 26802629
    15. Based on current evidence from published studies, neither of the two variants from KCNE was significantly associated with the risk of Meniere's disease--{REVIEW} PMID: 26890422
    16. Analysis of QT-RR relationship could also evaluate the latent arrhythmogenicity of KCNE1(G38S). PMID: 26520166
    17. The incidence of AF among the senior Uygur population in Xinjiang territory was correlated with the KCNE1 (G38S) polymorphism, which may be an independent risk factor for Uygur AF patients. PMID: 26662381
    18. The KCNQ1 F279I mutation induces a gain of function of IKs due to an impaired gating modulation of Kv7.1 induced by KCNE1, leading to a shortening of the cardiac action potential PMID: 26168993
    19. This study aimed to assess the associations between polymorphisms on KCNE1, KCNQ1, and KCNH2 with the risk of AF in a Chinese population. PMID: 26066992
    20. KCNE1 and KCNE3: The yin and yang of voltage-gated K(+) channel regulation PMID: 26410412
    21. Two phenylalanine residues on KCNQ1, Phe232 on S4 (VSD) and Phe279 on S5 (pore domain) to be responsible for the gating modulation by KCNE1. PMID: 24920132
    22. acute application of PUFAs increases Kv7.1/KCNE1 through a probably direct effect, and shows antiarrhythmic efficacy under IKr block. PMID: 25497550
    23. IKs phosphorylation and alpha1-AR regulation via activation of calcium-dependent PKC isoforms (cPKC) may be a key mechanism to control channel voltage-dependent activation and consequently action potential duration (APD) in response to adrenergic-stimulus. PMID: 25479336
    24. KCNE1 distal C-terminus appears essential for the regulation of yotiao-mediated PKA phosphorylation. PMID: 25037568
    25. the KCNE1 112G polymorphism may be a risk factor for AF. KCNE1 112G>A may be useful as a biomarker for predicting the development of AF. PMID: 25366730
    26. Klotho upregulates KCNQ1 and KCNE1 channel activity by enhancing channel protein abundance in the cell membrane. PMID: 24457979
    27. KCNE1-D85N is less stable than the wild-type protein, and is rapidly degraded through the ubiquitin-proteasome system. Verapamil may be of a therapeutic value in LQTS patients via preventing degradation of KCNE1-D85N PMID: 24499369
    28. The resilience of the curvature in the KCNE1 transmembrane domain is likely to be maintained upon binding of protein to the KCNQ1 channel. PMID: 25234231
    29. A significant association between Mink S38G gene polymorphism and AF risk was found. G allele carriers may predispose to AF PMID: 24696659
    30. A590T mutation in KCNQ1 C-terminal helix D decreases KCNE1 channel trafficking and function but not Yotiao interaction. PMID: 24713462
    31. Homozygous inheritance of KCNE1(38S) might cause a mild reduction of the delayed rectifier K(+) currents and might thereby increase an arrhythmogenic potential particularly in the presence of QT prolonging factors. PMID: 24419801
    32. KCNE1 divides the voltage sensor movement in KCNQ1/KCNE1 channels into two steps. PMID: 24769622
    33. The transmembrane domains (TMDs) of KCNE1 and KCNE2 were illustrated to associate with the KCNQ1 channel in different modes. PMID: 24827085
    34. study concluded that the variants in KCNQ1, KCNH2, KCNE1 and KCNE2 genes may be correlated with the occurrence of part of sudden unexplained nocturnal death syndrome cases in southern China PMID: 23890619
    35. This study confirmed the association of the 112G>A polymorphism and postoperative AF in a cohort of patients undergoing cardiac surgery. PMID: 24439990
    36. Subjects with LQT-PM may have longer QTc intervals at rest and at peak exercise and all phases of the recovery period compared to controls. Those with homozygous SNPs (KCNE1 35%) had longer resting QTc intervals when compared to heterozygotes. PMID: 23714088
    37. KCNE1 rs1805127 polymorphism increases the risk of atrial fibrillation. PMID: 23874724
    38. KCNE1 maintained a stable cell surface localization, whereas KCNQ1 exhibited variations in the cytosolic compartment (striations versus vesicles) and the degree of presence on the cell surface of cardiac myocytes PMID: 24142691
    39. KCNE1 single nucleotide polymorphism contributed to tinnitus that developed independently of hearing loss. PMID: 23224734
    40. KCNE1 gene abnormality and mutation is associated with long QT synrome development PMID: 23237912
    41. KCNE1 redirects the targeting-deficient KV7.1-Y51A mutant to the apical membrane of MDCK celsl. PMID: 23324056
    42. unsaturated heteromultimeric (KCNQ1)4(KCNE1)n channels are present as components of IKs and are pharmacologically distinct from KCNE-saturated KCNQ1-KCNE1 channels. PMID: 23650380
    43. KCNE1 and KCNE2, auxiliary subunits of voltage-gated potassium channels, undergo sequential cleavage mediated by either alpha-secretase and presenilin(PS)/gamma-secretase or BACE1 and PS/gamma-secretase in cells. PMID: 23504710
    44. The genetic polymorphism of KCNE1 was associated with increased risk of AF in a Chinese Han population. PMID: 23020083
    45. The KCNE1 G38S might have different impact on AF in different ethnicities. PMID: 23129484
    46. Screening of the genotype disclosed the KCNE1 D85N polymorphism, which is known as one of the typical disease-causing gene variants in long-QT syndrome (LQTS). PMID: 22999324
    47. IKs channels open slowly because KCNE1 accessory subunits slow the movement of S4 voltage sensors in KCNQ1 pore-forming subunits. PMID: 23359697
    48. KCNQ1 channels in both the absence and the presence of KCNE1 undergo sequential gating transitions leading to channel opening even before all VSDs have moved. PMID: 22908235
    49. Early-onset lone atrial fibrillation is associated with mutations in the potassium current channel regulatory subunit KCNE1. PMID: 22471742
    50. The KCNE1 variant rs1805128, resulting in D85N, confers substantially increased risk for drug-induced torsades de pointes. PMID: 22100668

    顯示更多

    收起更多

  • 相關(guān)疾病：
    Jervell and Lange-Nielsen syndrome 2 (JLNS2); Long QT syndrome 5 (LQT5)
  • 亞細(xì)胞定位：
    Cell membrane; Single-pass type I membrane protein. Apical cell membrane. Membrane raft.
  • 蛋白家族：
    Potassium channel KCNE family
  • 組織特異性：
    Expressed in lung, kidney, testis, ovaries, small intestine, peripheral blood leukocytes. Expressed in the heart. Not detected in pancreas, spleen, prostate and colon. Restrictively localized in the apical membrane portion of epithelial cells.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 6240

    OMIM: 176261

    KEGG: hsa:3753

    STRING: 9606.ENSP00000337255

    UniGene: Hs.121495