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  4. Recombinant Mouse Sialidase-4 (Neu4)

Recombinant Mouse Sialidase-4 (Neu4)

  • 中文名稱(chēng)：
    小鼠Neu4重組蛋白
  • 貨號(hào)：
    CSB-CF803970MO
  • 規(guī)格：
    ￥5268
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    Neu4
  • Uniprot No.：
  • 別名：
    Neu4; Sialidase-4; N-acetyl-alpha-neuraminidase 4; Neuraminidase 4
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長(zhǎng)度：
    Full Length
  • 來(lái)源：
    in vitro E.coli expression system
  • 表達(dá)區(qū)域：
    1-478aa
  • 氨基酸序列
    MGPTRVPRRTVLFQRERTGLTYRVPALLCVPPRPTLLAFAEQRLSPDDSHAHRLVLRRGTLTRGSVRWGTLSVLETAVLEEHRSMNPCPVLDEHSGTIFLFFIAVLGHTPEAVQIATGKNAARLCCVTSCDAGLTWGSVRDLTEEAIGAALQDWATFAVGPGHGVQLRSGRLLVPAYTYHVDRRECFGKICWTSPHSLAFYSDDHGISWHCGGLVPNLRSGECQLAAVDGDFLYCNARSPLGNRVQALSADEGTSFLPGELVPTLAETARGCQGSIVGFLAPPSIEPQDDRWTGSPRNTPHSPCFNLRVQESSGEGARGLLERWMPRLPLCYPQSRSPENHGLEPGSDGDKTSWTPECPMSSDSMLQSPTWLLYSHPAGRRARLHMGIYLSRSPLDPHSWTEPWVIYEGPSGYSDLAFLGPMPGASLVFACLFESGTRTSYEDISFCLFSLADVLENVPTGLEMLSLRDKAQGHCWPS
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標(biāo)簽：
    N-terminal 10xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產(chǎn)品描述：
    制備重組小鼠唾液酸酶-4 (Neu4)的一般步驟：
    獲取并克隆Neu4基因：從小鼠組織中提取總RNA，利用逆轉(zhuǎn)錄酶和PCR擴(kuò)增技術(shù)獲得Neu4基因。將擴(kuò)增后的Neu4基因插入到表達(dá)載體中，如pET系列載體中。
    轉(zhuǎn)染宿主細(xì)胞：將含有Neu4基因的表達(dá)載體轉(zhuǎn)染到宿主細(xì)胞中?？梢赃x擇常見(jiàn)的哺乳動(dòng)物細(xì)胞系，如CHO細(xì)胞、HEK293細(xì)胞等。
    表達(dá)Neu4蛋白：根據(jù)表達(dá)載體中的啟動(dòng)子和調(diào)節(jié)元件，誘導(dǎo)Neu4蛋白在轉(zhuǎn)染的宿主細(xì)胞中表達(dá)。在培養(yǎng)期間，需要添加抗生素等來(lái)保持表達(dá)載體的穩(wěn)定。
    收集細(xì)胞裂解液：利用細(xì)胞破碎和離心等方法，收集含有Neu4蛋白的細(xì)胞裂解液。
    純化Neu4蛋白：采用親和層析、離子交換層析、凝膠過(guò)濾層析等方法對(duì)Neu4蛋白進(jìn)行純化。
    鑒定和活性檢測(cè)：使用SDS-PAGE、Western blot等方法對(duì)純化后的Neu4蛋白進(jìn)行鑒定和定量。此外，可以使用唾液酸酶活性分析等方法來(lái)檢測(cè)其生物學(xué)活性和功能。
    需要注意的是，唾液酸酶是一種糖酶，需要在適當(dāng)?shù)膒H和溫度下進(jìn)行表達(dá)和純化。此外，唾液酸酶在儲(chǔ)存和使用時(shí)容易受到蛋白質(zhì)降解酶的影響，因此需要注意保存條件。
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Exo-alpha-sialidase that catalyzes the hydrolytic cleavage of the terminal sialic acid (N-acetylneuraminic acid, Neu5Ac) of a glycan moiety in the catabolism of glycolipids, glycoproteins and oligosacharides. Efficiently hydrolyzes gangliosides including alpha-(2->3)-sialylated GD1a and GM3 and alpha-(2->8)-sialylated GD3. Hydrolyzes poly-alpha-(2->8)-sialylated neural cell adhesion molecule NCAM1 likely at growth cones, suppressing neurite outgrowth in hippocampal neurons. May desialylate sialyl Lewis A and X antigens at the cell surface, down-regulating these glycan epitopes recognized by SELE/E selectin in the initiation of cell adhesion and extravasation. Has sialidase activity toward mucin, fetuin and sialyllactose.
  • 基因功能參考文獻(xiàn)：
    1. These results provide the first in vivo evidence that neuraminidases 3 and 4 have important roles in CNS function by catabolizing gangliosides and preventing their storage in lipofuscin bodies. PMID: 28442549
    2. NEU4 is involved in regulation of neuronal function by polySia degradation in mammals. PMID: 22393058
    3. Neu4 is not the only sialidase contributing to the metabolic bypass in Hexa(-/-) mice. PMID: 20862357
    4. Neu4 is a functional component of the ganglioside-metabolizing system, contributing to the postnatal development of the brain and other vital organs. PMID: 18270209
    5. mouse Neu4 plays an important regulatory role in neurite formation, possibly through desialylation of glycoproteins PMID: 19506080

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  • 亞細(xì)胞定位：
    Cell membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. Mitochondrion inner membrane; Peripheral membrane protein. Mitochondrion outer membrane; Peripheral membrane protein. Cell projection, neuron projection. Lysosome lumen.
  • 蛋白家族：
    Glycosyl hydrolase 33 family
  • 組織特異性：
    Highly expressed in brain, particularly in hippocampus, and at lower levels in liver and spleen. Expressed in hippocampal neurons (at protein level).
  • 數(shù)據(jù)庫(kù)鏈接：