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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Ralstonia pickettii 30S ribosomal protein S10 (rpsJ) CSB-YP458821RJI
CSB-EP458821RJI
CSB-BP458821RJI
CSB-MP458821RJI
CSB-EP458821RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii 50S ribosomal protein L10 (rplJ) CSB-YP458822RJI
CSB-EP458822RJI
CSB-BP458822RJI
CSB-MP458822RJI
CSB-EP458822RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii UPF0042 nucleotide-binding protein Rpic_0258 (Rpic_0258) CSB-YP458823RJI
CSB-EP458823RJI
CSB-BP458823RJI
CSB-MP458823RJI
CSB-EP458823RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Dihydroorotase (pyrC) CSB-YP458824RJI
CSB-EP458824RJI
CSB-BP458824RJI
CSB-MP458824RJI
CSB-EP458824RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP458825RJI
CSB-EP458825RJI
CSB-BP458825RJI
CSB-MP458825RJI
CSB-EP458825RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Bifunctional protein FolD (folD) CSB-YP458826RJI
CSB-EP458826RJI
CSB-BP458826RJI
CSB-MP458826RJI
CSB-EP458826RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP458827RJI
CSB-EP458827RJI
CSB-BP458827RJI
CSB-MP458827RJI
CSB-EP458827RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Chorismate synthase (aroC) CSB-YP458828RJI
CSB-EP458828RJI
CSB-BP458828RJI
CSB-MP458828RJI
CSB-EP458828RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Putative pterin-4-alpha-carbinolamine dehydratase (Rpic_3550) CSB-YP458829RJI
CSB-EP458829RJI
CSB-BP458829RJI
CSB-MP458829RJI
CSB-EP458829RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Probable 5-dehydro-4-deoxyglucarate dehydratase (Rpic_4452) CSB-YP458830RJI
CSB-EP458830RJI
CSB-BP458830RJI
CSB-MP458830RJI
CSB-EP458830RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP458831AZF
CSB-EP458831AZF
CSB-BP458831AZF
CSB-MP458831AZF
CSB-EP458831AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila 50S ribosomal protein L33 (rpmG) CSB-YP458832AZF
CSB-EP458832AZF
CSB-BP458832AZF
CSB-MP458832AZF
CSB-EP458832AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila Dihydrodipicolinate reductase (dapB) CSB-YP458833AZF
CSB-EP458833AZF
CSB-BP458833AZF
CSB-MP458833AZF
CSB-EP458833AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP458834AZF
CSB-EP458834AZF
CSB-BP458834AZF
CSB-MP458834AZF
CSB-EP458834AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila 30S ribosomal protein S19 (rpsS) CSB-YP458835AZF
CSB-EP458835AZF
CSB-BP458835AZF
CSB-MP458835AZF
CSB-EP458835AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP458836AZF
CSB-EP458836AZF
CSB-BP458836AZF
CSB-MP458836AZF
CSB-EP458836AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila Dihydroorotate dehydrogenase (quinone) CSB-YP458837AZF
CSB-EP458837AZF
CSB-BP458837AZF
CSB-MP458837AZF
CSB-EP458837AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila 30S ribosomal protein S21 (rpsU) CSB-YP458838AZF
CSB-EP458838AZF
CSB-BP458838AZF
CSB-MP458838AZF
CSB-EP458838AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila 30S ribosomal protein S13 (rpsM) CSB-YP458839AZF
CSB-EP458839AZF
CSB-BP458839AZF
CSB-MP458839AZF
CSB-EP458839AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP458840AZF
CSB-EP458840AZF
CSB-BP458840AZF
CSB-MP458840AZF
CSB-EP458840AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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