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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Yersinia pseudotuberculosis serotype IB Urease subunit gamma (ureA) CSB-YP460421YAW
CSB-EP460421YAW
CSB-BP460421YAW
CSB-MP460421YAW
CSB-EP460421YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elusimicrobium minutum Alanine--tRNA ligase (alaS), partial CSB-YP460422EKE
CSB-EP460422EKE
CSB-BP460422EKE
CSB-MP460422EKE
CSB-EP460422EKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elusimicrobium minutum 50S ribosomal protein L21 (rplU) CSB-YP460423EKE
CSB-EP460423EKE
CSB-BP460423EKE
CSB-MP460423EKE
CSB-EP460423EKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elusimicrobium minutum Aspartate--tRNA ligase (aspS), partial CSB-YP460424EKE
CSB-EP460424EKE
CSB-BP460424EKE
CSB-MP460424EKE
CSB-EP460424EKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elusimicrobium minutum UPF0234 protein Emin_0136 (Emin_0136) CSB-YP460425EKE
CSB-EP460425EKE
CSB-BP460425EKE
CSB-MP460425EKE
CSB-EP460425EKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elusimicrobium minutum Lon protease (lon), partial CSB-YP460426EKE
CSB-EP460426EKE
CSB-BP460426EKE
CSB-MP460426EKE
CSB-EP460426EKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elusimicrobium minutum SsrA-binding protein (smpB) CSB-YP460427EKE
CSB-EP460427EKE
CSB-BP460427EKE
CSB-MP460427EKE
CSB-EP460427EKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elusimicrobium minutum 10 kDa chaperonin (groS) CSB-YP460428EKE
CSB-EP460428EKE
CSB-BP460428EKE
CSB-MP460428EKE
CSB-EP460428EKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elusimicrobium minutum NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP460429EKE
CSB-EP460429EKE
CSB-BP460429EKE
CSB-MP460429EKE
CSB-EP460429EKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elusimicrobium minutum 50S ribosomal protein L25 (rplY) CSB-YP460430EKE
CSB-EP460430EKE
CSB-BP460430EKE
CSB-MP460430EKE
CSB-EP460430EKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elusimicrobium minutum 50S ribosomal protein L17 (rplQ) CSB-YP460431EKE
CSB-EP460431EKE
CSB-BP460431EKE
CSB-MP460431EKE
CSB-EP460431EKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elusimicrobium minutum 50S ribosomal protein L14 (rplN) CSB-YP460432EKE
CSB-EP460432EKE
CSB-BP460432EKE
CSB-MP460432EKE
CSB-EP460432EKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elusimicrobium minutum 50S ribosomal protein L2 (rplB) CSB-YP460433EKE
CSB-EP460433EKE
CSB-BP460433EKE
CSB-MP460433EKE
CSB-EP460433EKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elusimicrobium minutum NAD (P)H-hydrate epimerase CSB-YP460434EKE
CSB-EP460434EKE
CSB-BP460434EKE
CSB-MP460434EKE
CSB-EP460434EKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dendrobium findlayanum Mannose-specific lectin (dfa) CSB-YP460435DJE
CSB-EP460435DJE
CSB-BP460435DJE
CSB-MP460435DJE
CSB-EP460435DJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Guizotia abyssinica DNA-directed RNA polymerase subunit beta' (rpoC2), partial CSB-YP460436GHI
CSB-EP460436GHI
CSB-BP460436GHI
CSB-MP460436GHI
CSB-EP460436GHI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Guizotia abyssinica NAD (P)H-quinone oxidoreductase subunit K, chloroplastic CSB-YP460437GHI
CSB-EP460437GHI
CSB-BP460437GHI
CSB-MP460437GHI
CSB-EP460437GHI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Guizotia abyssinica 50S ribosomal protein L33, chloroplastic (rpl33) CSB-YP460438GHI
CSB-EP460438GHI
CSB-BP460438GHI
CSB-MP460438GHI
CSB-EP460438GHI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Guizotia abyssinica Protein psbN (psbN), partial CSB-YP460439GHI
CSB-EP460439GHI
CSB-BP460439GHI
CSB-MP460439GHI
CSB-EP460439GHI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Guizotia abyssinica 30S ribosomal protein S19, chloroplastic (rps19) CSB-YP460440GHI
CSB-EP460440GHI
CSB-BP460440GHI
CSB-MP460440GHI
CSB-EP460440GHI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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