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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Clostridium botulinum 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP460641DUG
CSB-EP460641DUG
CSB-BP460641DUG
CSB-MP460641DUG
CSB-EP460641DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Cell division topological specificity factor (minE) CSB-YP460642DUG
CSB-EP460642DUG
CSB-BP460642DUG
CSB-MP460642DUG
CSB-EP460642DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Biotin synthase (bioB) CSB-YP460643DUG
CSB-EP460643DUG
CSB-BP460643DUG
CSB-MP460643DUG
CSB-EP460643DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Flagellar assembly factor FliW (fliW) CSB-YP460644DUG
CSB-EP460644DUG
CSB-BP460644DUG
CSB-MP460644DUG
CSB-EP460644DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP460645DUG
CSB-EP460645DUG
CSB-BP460645DUG
CSB-MP460645DUG
CSB-EP460645DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Fructose-1,6-bisphosphatase class 3 (fbp), partial CSB-YP460646DUG
CSB-EP460646DUG
CSB-BP460646DUG
CSB-MP460646DUG
CSB-EP460646DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum HPr kinase/phosphorylase (hprK) CSB-YP460647DUG
CSB-EP460647DUG
CSB-BP460647DUG
CSB-MP460647DUG
CSB-EP460647DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Probable GTP-binding protein EngB (engB) CSB-YP460648DUG
CSB-EP460648DUG
CSB-BP460648DUG
CSB-MP460648DUG
CSB-EP460648DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Glutamyl-tRNA reductase (hemA) CSB-YP460649DUG
CSB-EP460649DUG
CSB-BP460649DUG
CSB-MP460649DUG
CSB-EP460649DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UPF0229 protein CLL_A3091 (CLL_A3091) CSB-YP460650DUG
CSB-EP460650DUG
CSB-BP460650DUG
CSB-MP460650DUG
CSB-EP460650DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP460651DUG
CSB-EP460651DUG
CSB-BP460651DUG
CSB-MP460651DUG
CSB-EP460651DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Tyrosine--tRNA ligase (tyrS) CSB-YP460652DUG
CSB-EP460652DUG
CSB-BP460652DUG
CSB-MP460652DUG
CSB-EP460652DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP460653DUG
CSB-EP460653DUG
CSB-BP460653DUG
CSB-MP460653DUG
CSB-EP460653DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP460654DUG
CSB-EP460654DUG
CSB-BP460654DUG
CSB-MP460654DUG
CSB-EP460654DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP460655DUG
CSB-EP460655DUG
CSB-BP460655DUG
CSB-MP460655DUG
CSB-EP460655DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 33 kDa chaperonin (hslO) CSB-YP460656DUG
CSB-EP460656DUG
CSB-BP460656DUG
CSB-MP460656DUG
CSB-EP460656DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Cysteine--tRNA ligase (cysS) CSB-YP460657SYZ
CSB-EP460657SYZ
CSB-BP460657SYZ
CSB-MP460657SYZ
CSB-EP460657SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Probable phosphatase YcdX (ycdX) CSB-YP460658SYZ
CSB-EP460658SYZ
CSB-BP460658SYZ
CSB-MP460658SYZ
CSB-EP460658SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 UPF0319 protein YccT (yccT) CSB-YP460659SYZ
CSB-EP460659SYZ
CSB-BP460659SYZ
CSB-MP460659SYZ
CSB-EP460659SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 UPF0758 protein yicR (yicR) CSB-YP460660SYZ
CSB-EP460660SYZ
CSB-BP460660SYZ
CSB-MP460660SYZ
CSB-EP460660SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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