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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Phenylobacterium zucineum Dihydrodipicolinate synthase (dapA) CSB-YP461641EUP
CSB-EP461641EUP
CSB-BP461641EUP
CSB-MP461641EUP
CSB-EP461641EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum 50S ribosomal protein L27 (rpmA) CSB-YP461642EUP
CSB-EP461642EUP
CSB-BP461642EUP
CSB-MP461642EUP
CSB-EP461642EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum Recombination protein RecR (recR) CSB-YP461643EUP
CSB-EP461643EUP
CSB-BP461643EUP
CSB-MP461643EUP
CSB-EP461643EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum Bifunctional protein FolD (folD) CSB-YP461644EUP
CSB-EP461644EUP
CSB-BP461644EUP
CSB-MP461644EUP
CSB-EP461644EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP461645EUP
CSB-EP461645EUP
CSB-BP461645EUP
CSB-MP461645EUP
CSB-EP461645EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP461646EUP
CSB-EP461646EUP
CSB-BP461646EUP
CSB-MP461646EUP
CSB-EP461646EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum Aspartate 1-decarboxylase (panD) CSB-YP461647EUP
CSB-EP461647EUP
CSB-BP461647EUP
CSB-MP461647EUP
CSB-EP461647EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum Lon protease (lon), partial CSB-YP461648EUP
CSB-EP461648EUP
CSB-BP461648EUP
CSB-MP461648EUP
CSB-EP461648EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae Histidinol-phosphate aminotransferase (hisC) CSB-YP461649NGB
CSB-EP461649NGB
CSB-BP461649NGB
CSB-MP461649NGB
CSB-EP461649NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP461650NGB
CSB-EP461650NGB
CSB-BP461650NGB
CSB-MP461650NGB
CSB-EP461650NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae Ribonuclease P protein component (rnpA) CSB-YP461651NGB
CSB-EP461651NGB
CSB-BP461651NGB
CSB-MP461651NGB
CSB-EP461651NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae UPF0434 protein NGK_0378 (NGK_0378) CSB-YP461652NGB
CSB-EP461652NGB
CSB-BP461652NGB
CSB-MP461652NGB
CSB-EP461652NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae N- (5'-phosphoribosyl)anthranilate isomerase CSB-YP461653NGB
CSB-EP461653NGB
CSB-BP461653NGB
CSB-MP461653NGB
CSB-EP461653NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae UPF0042 nucleotide-binding protein NGK_0463 (NGK_0463) CSB-YP461654NGB
CSB-EP461654NGB
CSB-BP461654NGB
CSB-MP461654NGB
CSB-EP461654NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae Peptidyl-tRNA hydrolase (pth) CSB-YP461655NGB
CSB-EP461655NGB
CSB-BP461655NGB
CSB-MP461655NGB
CSB-EP461655NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP461656NGB
CSB-EP461656NGB
CSB-BP461656NGB
CSB-MP461656NGB
CSB-EP461656NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP461657NGB
CSB-EP461657NGB
CSB-BP461657NGB
CSB-MP461657NGB
CSB-EP461657NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae 50S ribosomal protein L31 type B (rpmE2) CSB-YP461658NGB
CSB-EP461658NGB
CSB-BP461658NGB
CSB-MP461658NGB
CSB-EP461658NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP461659NGB1
CSB-EP461659NGB1
CSB-BP461659NGB1
CSB-MP461659NGB1
CSB-EP461659NGB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae UPF0758 protein NGK_1225 (NGK_1225) CSB-YP461660NGB
CSB-EP461660NGB
CSB-BP461660NGB
CSB-MP461660NGB
CSB-EP461660NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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