Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rhodopseudomonas palustris tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP465162RLV
CSB-EP465162RLV
CSB-BP465162RLV
CSB-MP465162RLV
CSB-EP465162RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris UPF0314 protein Rpal_0309 (Rpal_0309), partial CSB-YP465163RLV1
CSB-EP465163RLV1
CSB-BP465163RLV1
CSB-MP465163RLV1
CSB-EP465163RLV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Potassium-transporting ATPase C chain (kdpC), partial CSB-YP465164RLV1
CSB-EP465164RLV1
CSB-BP465164RLV1
CSB-MP465164RLV1
CSB-EP465164RLV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Isocitrate dehydrogenase kinase/phosphatase (aceK), partial CSB-YP465165RLV
CSB-EP465165RLV
CSB-BP465165RLV
CSB-MP465165RLV
CSB-EP465165RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris UPF0317 protein Rpal_1947 (Rpal_1947) CSB-YP465166RLV
CSB-EP465166RLV
CSB-BP465166RLV
CSB-MP465166RLV
CSB-EP465166RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Polyphosphate kinase (ppk), partial CSB-YP465167RLV
CSB-EP465167RLV
CSB-BP465167RLV
CSB-MP465167RLV
CSB-EP465167RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP465168RLV
CSB-EP465168RLV
CSB-BP465168RLV
CSB-MP465168RLV
CSB-EP465168RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris UPF0262 protein Rpal_5021 (Rpal_5021) CSB-YP465169RLV
CSB-EP465169RLV
CSB-BP465169RLV
CSB-MP465169RLV
CSB-EP465169RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (fabA) CSB-YP465170RLV
CSB-EP465170RLV
CSB-BP465170RLV
CSB-MP465170RLV
CSB-EP465170RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Oxaloacetate decarboxylase (Rpal_3584) CSB-YP465171RLV
CSB-EP465171RLV
CSB-BP465171RLV
CSB-MP465171RLV
CSB-EP465171RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Monofunctional biosynthetic peptidoglycan transglycosylase (mtgA), partial CSB-YP465172RLV1
CSB-EP465172RLV1
CSB-BP465172RLV1
CSB-MP465172RLV1
CSB-EP465172RLV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Pyrroloquinoline-quinone synthase (pqqC) CSB-YP465173RLV
CSB-EP465173RLV
CSB-BP465173RLV
CSB-MP465173RLV
CSB-EP465173RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 50S ribosomal protein L15 (rplO) CSB-YP465174RLV
CSB-EP465174RLV
CSB-BP465174RLV
CSB-MP465174RLV
CSB-EP465174RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 30S ribosomal protein S14 (rpsN) CSB-YP465175RLV
CSB-EP465175RLV
CSB-BP465175RLV
CSB-MP465175RLV
CSB-EP465175RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 50S ribosomal protein L10 (rplJ) CSB-YP465176RLV
CSB-EP465176RLV
CSB-BP465176RLV
CSB-MP465176RLV
CSB-EP465176RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Protein ApaG (apaG) CSB-YP465177RLV
CSB-EP465177RLV
CSB-BP465177RLV
CSB-MP465177RLV
CSB-EP465177RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Molybdenum cofactor biosynthesis protein A (moaA) CSB-YP465178RLV
CSB-EP465178RLV
CSB-BP465178RLV
CSB-MP465178RLV
CSB-EP465178RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 2-isopropylmalate synthase (leuA), partial CSB-YP465179RLV
CSB-EP465179RLV
CSB-BP465179RLV
CSB-MP465179RLV
CSB-EP465179RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Putative membrane protein insertion efficiency factor (Rpal_3875) CSB-YP465180RLV
CSB-EP465180RLV
CSB-BP465180RLV
CSB-MP465180RLV
CSB-EP465180RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Cytochrome c oxidase assembly protein CtaG (ctaG), partial CSB-YP465181RLV
CSB-EP465181RLV
CSB-BP465181RLV
CSB-MP465181RLV
CSB-EP465181RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>