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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Escherichia coli O157:H7 D-erythrose-4-phosphate dehydrogenase (epd) CSB-YP466763EOE
CSB-EP466763EOE
CSB-BP466763EOE
CSB-MP466763EOE
CSB-EP466763EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Octanoyltransferase (lipB) CSB-YP466764EOE
CSB-EP466764EOE
CSB-BP466764EOE
CSB-MP466764EOE
CSB-EP466764EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Potassium-transporting ATPase A chain (kdpA), partial CSB-YP466765EOE
CSB-EP466765EOE
CSB-BP466765EOE
CSB-MP466765EOE
CSB-EP466765EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Uncharacterized Nudix hydrolase NudL (nudL) CSB-YP466766EOE
CSB-EP466766EOE
CSB-BP466766EOE
CSB-MP466766EOE
CSB-EP466766EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 tRNA (cmo5U34)-methyltransferase CSB-YP466767EOE
CSB-EP466767EOE
CSB-BP466767EOE
CSB-MP466767EOE
CSB-EP466767EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Glycogen synthesis protein glgS (glgS) CSB-YP466768EOE
CSB-EP466768EOE
CSB-BP466768EOE
CSB-MP466768EOE
CSB-EP466768EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 G/U mismatch-specific DNA glycosylase (mug) CSB-YP466769EOE
CSB-EP466769EOE
CSB-BP466769EOE
CSB-MP466769EOE
CSB-EP466769EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Protein TolB (tolB) CSB-YP466770EOE
CSB-EP466770EOE
CSB-BP466770EOE
CSB-MP466770EOE
CSB-EP466770EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UvrABC system protein B (uvrB) CSB-YP466771EOE
CSB-EP466771EOE
CSB-BP466771EOE
CSB-MP466771EOE
CSB-EP466771EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 D-tagatose-1,6-bisphosphate aldolase subunit KbaY (kbaY) CSB-YP466772EOE
CSB-EP466772EOE
CSB-BP466772EOE
CSB-MP466772EOE
CSB-EP466772EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 30S ribosomal protein S15 (rpsO) CSB-YP466773EOE
CSB-EP466773EOE
CSB-BP466773EOE
CSB-MP466773EOE
CSB-EP466773EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 DNA protection during starvation protein (dps) CSB-YP466774EOE
CSB-EP466774EOE
CSB-BP466774EOE
CSB-MP466774EOE
CSB-EP466774EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Putative transport protein YbjL (ybjL), partial CSB-YP466775EOE
CSB-EP466775EOE
CSB-BP466775EOE
CSB-MP466775EOE
CSB-EP466775EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP466776EOE
CSB-EP466776EOE
CSB-BP466776EOE
CSB-MP466776EOE
CSB-EP466776EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Sulfoxide reductase catalytic subunit YedY (yedY) CSB-YP466777EOE
CSB-EP466777EOE
CSB-BP466777EOE
CSB-MP466777EOE
CSB-EP466777EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Cobalamin synthase (cobS), partial CSB-YP466778EOE1
CSB-EP466778EOE1
CSB-BP466778EOE1
CSB-MP466778EOE1
CSB-EP466778EOE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Putative sialic acid transporter (nanT), partial CSB-YP466779EOE
CSB-EP466779EOE
CSB-BP466779EOE
CSB-MP466779EOE
CSB-EP466779EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 p-hydroxybenzoic acid efflux pump subunit AaeB (aaeB), partial CSB-YP466780EOE
CSB-EP466780EOE
CSB-BP466780EOE
CSB-MP466780EOE
CSB-EP466780EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Ribosomal RNA small subunit methyltransferase B (rsmB) CSB-YP466781EOE
CSB-EP466781EOE
CSB-BP466781EOE
CSB-MP466781EOE
CSB-EP466781EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 30S ribosomal protein S4 (rpsD) CSB-YP466782EOE
CSB-EP466782EOE
CSB-BP466782EOE
CSB-MP466782EOE
CSB-EP466782EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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