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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Coxiella burnetii tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP471864DXM
CSB-EP471864DXM
CSB-BP471864DXM
CSB-MP471864DXM
CSB-EP471864DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Probable disulfide formation protein (CbuG_1114), partial CSB-YP471865DXM1
CSB-EP471865DXM1
CSB-BP471865DXM1
CSB-MP471865DXM1
CSB-EP471865DXM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP471866DXM
CSB-EP471866DXM
CSB-BP471866DXM
CSB-MP471866DXM
CSB-EP471866DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Thiosulfate sulfurtransferase glpE (glpE) CSB-YP471867DXM
CSB-EP471867DXM
CSB-BP471867DXM
CSB-MP471867DXM
CSB-EP471867DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Phosphate acyltransferase (plsX) CSB-YP471868DXM
CSB-EP471868DXM
CSB-BP471868DXM
CSB-MP471868DXM
CSB-EP471868DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Ribosome maturation factor RimM (rimM) CSB-YP471869DXM
CSB-EP471869DXM
CSB-BP471869DXM
CSB-MP471869DXM
CSB-EP471869DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Pantothenate synthetase (panC) CSB-YP471870DXM
CSB-EP471870DXM
CSB-BP471870DXM
CSB-MP471870DXM
CSB-EP471870DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii GTPase obg (obg) CSB-YP471871DXM
CSB-EP471871DXM
CSB-BP471871DXM
CSB-MP471871DXM
CSB-EP471871DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP471872DXM
CSB-EP471872DXM
CSB-BP471872DXM
CSB-MP471872DXM
CSB-EP471872DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 50S ribosomal protein L17 (rplQ) CSB-YP471873DXM
CSB-EP471873DXM
CSB-BP471873DXM
CSB-MP471873DXM
CSB-EP471873DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 50S ribosomal protein L15 (rplO) CSB-YP471874DXM
CSB-EP471874DXM
CSB-BP471874DXM
CSB-MP471874DXM
CSB-EP471874DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 50S ribosomal protein L29 (rpmC) CSB-YP471875DXM
CSB-EP471875DXM
CSB-BP471875DXM
CSB-MP471875DXM
CSB-EP471875DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 50S ribosomal protein L3 (rplC) CSB-YP471876DXM
CSB-EP471876DXM
CSB-BP471876DXM
CSB-MP471876DXM
CSB-EP471876DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 50S ribosomal protein L1 (rplA) CSB-YP471877DXM
CSB-EP471877DXM
CSB-BP471877DXM
CSB-MP471877DXM
CSB-EP471877DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP471878DXM
CSB-EP471878DXM
CSB-BP471878DXM
CSB-MP471878DXM
CSB-EP471878DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Glutamate--tRNA ligase 2 (gltX2) CSB-YP471879DXM
CSB-EP471879DXM
CSB-BP471879DXM
CSB-MP471879DXM
CSB-EP471879DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP471880DXM
CSB-EP471880DXM
CSB-BP471880DXM
CSB-MP471880DXM
CSB-EP471880DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 30S ribosomal protein S9 (rpsI) CSB-YP471881DXM
CSB-EP471881DXM
CSB-BP471881DXM
CSB-MP471881DXM
CSB-EP471881DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 50S ribosomal protein L25 (rplY) CSB-YP471882DXM
CSB-EP471882DXM
CSB-BP471882DXM
CSB-MP471882DXM
CSB-EP471882DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Bis (5'-nucleosyl)-tetraphosphatase, symmetrical CSB-YP471883DXM
CSB-EP471883DXM
CSB-BP471883DXM
CSB-MP471883DXM
CSB-EP471883DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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