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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Coxiella burnetii Tryptophan synthase alpha chain (trpA) CSB-YP471904DXN
CSB-EP471904DXN
CSB-BP471904DXN
CSB-MP471904DXN
CSB-EP471904DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Chaperone protein DnaK (dnaK), partial CSB-YP471905DXN
CSB-EP471905DXN
CSB-BP471905DXN
CSB-MP471905DXN
CSB-EP471905DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP471906DXN
CSB-EP471906DXN
CSB-BP471906DXN
CSB-MP471906DXN
CSB-EP471906DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 50S ribosomal protein L32 (rpmF) CSB-YP471907DXN
CSB-EP471907DXN
CSB-BP471907DXN
CSB-MP471907DXN
CSB-EP471907DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Ribosome maturation factor RimM (rimM) CSB-YP471908DXN
CSB-EP471908DXN
CSB-BP471908DXN
CSB-MP471908DXN
CSB-EP471908DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Chromosomal replication initiator protein DnaA (dnaA) CSB-YP471909DXN
CSB-EP471909DXN
CSB-BP471909DXN
CSB-MP471909DXN
CSB-EP471909DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP471910DXN
CSB-EP471910DXN
CSB-BP471910DXN
CSB-MP471910DXN
CSB-EP471910DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 50S ribosomal protein L25 (rplY) CSB-YP471911DXN
CSB-EP471911DXN
CSB-BP471911DXN
CSB-MP471911DXN
CSB-EP471911DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Pantothenate synthetase (panC) CSB-YP471912DXN
CSB-EP471912DXN
CSB-BP471912DXN
CSB-MP471912DXN
CSB-EP471912DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 50S ribosomal protein L27 (rpmA) CSB-YP471913DXN
CSB-EP471913DXN
CSB-BP471913DXN
CSB-MP471913DXN
CSB-EP471913DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP471914OED
CSB-EP471914OED
CSB-BP471914OED
CSB-MP471914OED
CSB-EP471914OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Putative phosphotransferase OCAR_4385/OCA5_c01460 (OCAR_4385, OCA5_c01460) CSB-YP471915OED
CSB-EP471915OED
CSB-BP471915OED
CSB-MP471915OED
CSB-EP471915OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans 30S ribosomal protein S16 (rpsP) CSB-YP471916OED
CSB-EP471916OED
CSB-BP471916OED
CSB-MP471916OED
CSB-EP471916OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans D-alanine--D-alanine ligase (ddl) CSB-YP471917OED
CSB-EP471917OED
CSB-BP471917OED
CSB-MP471917OED
CSB-EP471917OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans 30S ribosomal protein S21 (rpsU) CSB-YP471918OED
CSB-EP471918OED
CSB-BP471918OED
CSB-MP471918OED
CSB-EP471918OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP471919OED
CSB-EP471919OED
CSB-BP471919OED
CSB-MP471919OED
CSB-EP471919OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Dihydrodipicolinate reductase (dapB) CSB-YP471920OED
CSB-EP471920OED
CSB-BP471920OED
CSB-MP471920OED
CSB-EP471920OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Cytidylate kinase (cmk) CSB-YP471921OED
CSB-EP471921OED
CSB-BP471921OED
CSB-MP471921OED
CSB-EP471921OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans N- (5'-phosphoribosyl)anthranilate isomerase CSB-YP471922OED
CSB-EP471922OED
CSB-BP471922OED
CSB-MP471922OED
CSB-EP471922OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP471923OED
CSB-EP471923OED
CSB-BP471923OED
CSB-MP471923OED
CSB-EP471923OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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