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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus cereus 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP478666BQL
CSB-EP478666BQL
CSB-BP478666BQL
CSB-MP478666BQL
CSB-EP478666BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L7/L12 (rplL) CSB-YP478667BQL
CSB-EP478667BQL
CSB-BP478667BQL
CSB-MP478667BQL
CSB-EP478667BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L2 (rplB) CSB-YP478668BQL
CSB-EP478668BQL
CSB-BP478668BQL
CSB-MP478668BQL
CSB-EP478668BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S19 (rpsS) CSB-YP478669BQL
CSB-EP478669BQL
CSB-BP478669BQL
CSB-MP478669BQL
CSB-EP478669BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L24 (rplX) CSB-YP478670BQL
CSB-EP478670BQL
CSB-BP478670BQL
CSB-MP478670BQL
CSB-EP478670BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Adenylate kinase (adk) CSB-YP478671BQL
CSB-EP478671BQL
CSB-BP478671BQL
CSB-MP478671BQL
CSB-EP478671BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S9 (rpsI) CSB-YP478672BQL
CSB-EP478672BQL
CSB-BP478672BQL
CSB-MP478672BQL
CSB-EP478672BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Heme-degrading monooxygenase isdG (isdG) CSB-YP478673BQL
CSB-EP478673BQL
CSB-BP478673BQL
CSB-MP478673BQL
CSB-EP478673BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L20 (rplT) CSB-YP478674BQL
CSB-EP478674BQL
CSB-BP478674BQL
CSB-MP478674BQL
CSB-EP478674BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Protein sprT-like (BCAH187_A0290) CSB-YP478675BQL
CSB-EP478675BQL
CSB-BP478675BQL
CSB-MP478675BQL
CSB-EP478675BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP478676BQL
CSB-EP478676BQL
CSB-BP478676BQL
CSB-MP478676BQL
CSB-EP478676BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0397 protein BCAH187_A2708 (BCAH187_A2708), partial CSB-YP478677BQL1
CSB-EP478677BQL1
CSB-BP478677BQL1
CSB-MP478677BQL1
CSB-EP478677BQL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP478678BQL
CSB-EP478678BQL
CSB-BP478678BQL
CSB-MP478678BQL
CSB-EP478678BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphoenolpyruvate carboxykinase [ATP] (pckA), partial CSB-YP478679BQL
CSB-EP478679BQL
CSB-BP478679BQL
CSB-MP478679BQL
CSB-EP478679BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP478680BQL
CSB-EP478680BQL
CSB-BP478680BQL
CSB-MP478680BQL
CSB-EP478680BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Putative membrane protein insertion efficiency factor (BCAH187_A4932) CSB-YP478681BQL
CSB-EP478681BQL
CSB-BP478681BQL
CSB-MP478681BQL
CSB-EP478681BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Regulatory protein recX (recX) CSB-YP478682BQL
CSB-EP478682BQL
CSB-BP478682BQL
CSB-MP478682BQL
CSB-EP478682BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable manganese-dependent inorganic pyrophosphatase (ppaC) CSB-YP478683BQL
CSB-EP478683BQL
CSB-BP478683BQL
CSB-MP478683BQL
CSB-EP478683BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable malate:quinone oxidoreductase (mqo) CSB-YP478684BQL
CSB-EP478684BQL
CSB-BP478684BQL
CSB-MP478684BQL
CSB-EP478684BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0178 protein BCAH187_A3092 (BCAH187_A3092) CSB-YP478685BQL
CSB-EP478685BQL
CSB-BP478685BQL
CSB-MP478685BQL
CSB-EP478685BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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