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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus cereus 50S ribosomal protein L29 (rpmC) CSB-YP478626BQO
CSB-EP478626BQO
CSB-BP478626BQO
CSB-MP478626BQO
CSB-EP478626BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L14 (rplN) CSB-YP478627BQO
CSB-EP478627BQO
CSB-BP478627BQO
CSB-MP478627BQO
CSB-EP478627BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Adenylate kinase (adk) CSB-YP478628BQO
CSB-EP478628BQO
CSB-BP478628BQO
CSB-MP478628BQO
CSB-EP478628BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus NH (3)-dependent NAD (+) synthetase CSB-YP478629BQO
CSB-EP478629BQO
CSB-BP478629BQO
CSB-MP478629BQO
CSB-EP478629BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphonoacetaldehyde hydrolase (phnX) CSB-YP478630BQL
CSB-EP478630BQL
CSB-BP478630BQL
CSB-MP478630BQL
CSB-EP478630BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus D-alanine--poly (phosphoribitol) ligase subunit 1, partial CSB-YP478631BQL
CSB-EP478631BQL
CSB-BP478631BQL
CSB-MP478631BQL
CSB-EP478631BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Imidazole glycerol phosphate synthase subunit HisH (hisH) CSB-YP478632BQL
CSB-EP478632BQL
CSB-BP478632BQL
CSB-MP478632BQL
CSB-EP478632BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Sulfate adenylyltransferase (sat) CSB-YP478633BQL
CSB-EP478633BQL
CSB-BP478633BQL
CSB-MP478633BQL
CSB-EP478633BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0154 protein BCAH187_A3740 (BCAH187_A3740), partial CSB-YP478634BQL
CSB-EP478634BQL
CSB-BP478634BQL
CSB-MP478634BQL
CSB-EP478634BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Isopentenyl-diphosphate delta-isomerase (fni) CSB-YP478635BQL
CSB-EP478635BQL
CSB-BP478635BQL
CSB-MP478635BQL
CSB-EP478635BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 3-dehydroquinate synthase (aroB) CSB-YP478636BQL
CSB-EP478636BQL
CSB-BP478636BQL
CSB-MP478636BQL
CSB-EP478636BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Aspartate 1-decarboxylase (panD) CSB-YP478637BQL
CSB-EP478637BQL
CSB-BP478637BQL
CSB-MP478637BQL
CSB-EP478637BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Cell cycle protein GpsB (gpsB) CSB-YP478638BQL
CSB-EP478638BQL
CSB-BP478638BQL
CSB-MP478638BQL
CSB-EP478638BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP478639BQL
CSB-EP478639BQL
CSB-BP478639BQL
CSB-MP478639BQL
CSB-EP478639BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Methylenetetrahydrofolate--tRNA- (uracil-5-)-methyltransferase TrmFO CSB-YP478640BQL
CSB-EP478640BQL
CSB-BP478640BQL
CSB-MP478640BQL
CSB-EP478640BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S16 (rpsP) CSB-YP478641BQL
CSB-EP478641BQL
CSB-BP478641BQL
CSB-MP478641BQL
CSB-EP478641BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L28 (rpmB) CSB-YP478642BQL
CSB-EP478642BQL
CSB-BP478642BQL
CSB-MP478642BQL
CSB-EP478642BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP478643BQL
CSB-EP478643BQL
CSB-BP478643BQL
CSB-MP478643BQL
CSB-EP478643BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Bifunctional protein pyrR (pyrR) CSB-YP478644BQL
CSB-EP478644BQL
CSB-BP478644BQL
CSB-MP478644BQL
CSB-EP478644BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0747 protein BCAH187_A3972 (BCAH187_A3972), partial CSB-YP478645BQL
CSB-EP478645BQL
CSB-BP478645BQL
CSB-MP478645BQL
CSB-EP478645BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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