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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli O17:K52:H18 Chorismate--pyruvate lyase (ubiC) CSB-YP482881EOG
CSB-EP482881EOG
CSB-BP482881EOG
CSB-MP482881EOG
CSB-EP482881EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 10 kDa chaperonin (groS) CSB-YP482882EOG
CSB-EP482882EOG
CSB-BP482882EOG
CSB-MP482882EOG
CSB-EP482882EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 HTH-type transcriptional regulator UlaR (ulaR) CSB-YP482883EOG
CSB-EP482883EOG
CSB-BP482883EOG
CSB-MP482883EOG
CSB-EP482883EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 30S ribosomal protein S18 (rpsR) CSB-YP482884EOG
CSB-EP482884EOG
CSB-BP482884EOG
CSB-MP482884EOG
CSB-EP482884EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Phosphoglycerol transferase I (mdoB), partial CSB-YP482885EOG1
CSB-EP482885EOG1
CSB-BP482885EOG1
CSB-MP482885EOG1
CSB-EP482885EOG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Trp operon repressor (trpR) CSB-YP482886EOG
CSB-EP482886EOG
CSB-BP482886EOG
CSB-MP482886EOG
CSB-EP482886EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O7:K1 Chaperone protein DnaJ (dnaJ) CSB-YP482887EON
CSB-EP482887EON
CSB-BP482887EON
CSB-MP482887EON
CSB-EP482887EON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O7:K1 Probable crotonobetaine/carnitine-CoA ligase (caiC) CSB-YP482888EON
CSB-EP482888EON
CSB-BP482888EON
CSB-MP482888EON
CSB-EP482888EON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O7:K1 Glutathione-regulated potassium-efflux system protein kefC (kefC), partial CSB-YP482889EON
CSB-EP482889EON
CSB-BP482889EON
CSB-MP482889EON
CSB-EP482889EON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O7:K1 Ribulokinase (araB) CSB-YP482890EON
CSB-EP482890EON
CSB-BP482890EON
CSB-MP482890EON
CSB-EP482890EON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O7:K1 UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP482891EON
CSB-EP482891EON
CSB-BP482891EON
CSB-MP482891EON
CSB-EP482891EON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O7:K1 UPF0289 protein yacF (yacF) CSB-YP482892EON
CSB-EP482892EON
CSB-BP482892EON
CSB-MP482892EON
CSB-EP482892EON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O7:K1 UPF0267 protein yqfB (yqfB) CSB-YP482893EON
CSB-EP482893EON
CSB-BP482893EON
CSB-MP482893EON
CSB-EP482893EON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O7:K1 D-erythrose-4-phosphate dehydrogenase (epd) CSB-YP482894EON
CSB-EP482894EON
CSB-BP482894EON
CSB-MP482894EON
CSB-EP482894EON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O7:K1 Probable Fe (2+)-trafficking protein CSB-YP482895EON
CSB-EP482895EON
CSB-BP482895EON
CSB-MP482895EON
CSB-EP482895EON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O7:K1 Pantothenate synthetase (panC) CSB-YP482896EON
CSB-EP482896EON
CSB-BP482896EON
CSB-MP482896EON
CSB-EP482896EON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O7:K1 Deoxyguanosinetriphosphate triphosphohydrolase (dgt) CSB-YP482897EON
CSB-EP482897EON
CSB-BP482897EON
CSB-MP482897EON
CSB-EP482897EON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O7:K1 Acetyl esterase (aes) CSB-YP482898EON
CSB-EP482898EON
CSB-BP482898EON
CSB-MP482898EON
CSB-EP482898EON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O7:K1 Trans-aconitate 2-methyltransferase (tam) CSB-YP482899EON
CSB-EP482899EON
CSB-BP482899EON
CSB-MP482899EON
CSB-EP482899EON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O7:K1 ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP482900EON
CSB-EP482900EON
CSB-BP482900EON
CSB-MP482900EON
CSB-EP482900EON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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