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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli O127:H6 Mannonate dehydratase (uxuA) CSB-YP483081EOB
CSB-EP483081EOB
CSB-BP483081EOB
CSB-MP483081EOB
CSB-EP483081EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 UPF0391 membrane protein ytjA (ytjA), partial CSB-YP483082EOB
CSB-EP483082EOB
CSB-BP483082EOB
CSB-MP483082EOB
CSB-EP483082EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Trp operon repressor (trpR) CSB-YP483083EOB
CSB-EP483083EOB
CSB-BP483083EOB
CSB-MP483083EOB
CSB-EP483083EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 UPF0283 membrane protein ycjF (ycjF), partial CSB-YP483084EOB1
CSB-EP483084EOB1
CSB-BP483084EOB1
CSB-MP483084EOB1
CSB-EP483084EOB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Spermidine export protein MdtI (mdtI), partial CSB-YP483085EOB1
CSB-EP483085EOB1
CSB-BP483085EOB1
CSB-MP483085EOB1
CSB-EP483085EOB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Electron transport complex protein RnfC (rnfC) CSB-YP483086EOB
CSB-EP483086EOB
CSB-BP483086EOB
CSB-MP483086EOB
CSB-EP483086EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 HTH-type transcriptional repressor PurR (purR) CSB-YP483087EOB
CSB-EP483087EOB
CSB-BP483087EOB
CSB-MP483087EOB
CSB-EP483087EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Putative phosphotransferase ydiA (ydiA) CSB-YP483088EOB
CSB-EP483088EOB
CSB-BP483088EOB
CSB-MP483088EOB
CSB-EP483088EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP483089EOB
CSB-EP483089EOB
CSB-BP483089EOB
CSB-MP483089EOB
CSB-EP483089EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Threonine--tRNA ligase (thrS) CSB-YP483090EOB
CSB-EP483090EOB
CSB-BP483090EOB
CSB-MP483090EOB
CSB-EP483090EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 N-succinylglutamate 5-semialdehyde dehydrogenase (astD) CSB-YP483091EOB
CSB-EP483091EOB
CSB-BP483091EOB
CSB-MP483091EOB
CSB-EP483091EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 UPF0181 protein yoaH (yoaH) CSB-YP483092EOB
CSB-EP483092EOB
CSB-BP483092EOB
CSB-MP483092EOB
CSB-EP483092EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 ProP effector (proQ) CSB-YP483093EOB
CSB-EP483093EOB
CSB-BP483093EOB
CSB-MP483093EOB
CSB-EP483093EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Copper homeostasis protein CutC (cutC) CSB-YP483094EOB
CSB-EP483094EOB
CSB-BP483094EOB
CSB-MP483094EOB
CSB-EP483094EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Sulfoxide reductase heme-binding subunit YedZ (yedZ), partial CSB-YP483095EOB1
CSB-EP483095EOB1
CSB-BP483095EOB1
CSB-MP483095EOB1
CSB-EP483095EOB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Histidinol-phosphate aminotransferase (hisC) CSB-YP483096EOB
CSB-EP483096EOB
CSB-BP483096EOB
CSB-MP483096EOB
CSB-EP483096EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Probable lipid kinase YegS (yegS) CSB-YP483097EOB
CSB-EP483097EOB
CSB-BP483097EOB
CSB-MP483097EOB
CSB-EP483097EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Peptide methionine sulfoxide reductase MsrB (msrB) CSB-YP483098EZY
CSB-EP483098EZY
CSB-BP483098EZY
CSB-MP483098EZY
CSB-EP483098EZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Bacteriohemerythrin (PLES_36541) CSB-YP483099EZY
CSB-EP483099EZY
CSB-BP483099EZY
CSB-MP483099EZY
CSB-EP483099EZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Cell division protein ZipA homolog (zipA), partial CSB-YP483100EZY
CSB-EP483100EZY
CSB-BP483100EZY
CSB-MP483100EZY
CSB-EP483100EZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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