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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Escherichia coli O127:H6 Peptidase B (pepB) CSB-YP483001EOB
CSB-EP483001EOB
CSB-BP483001EOB
CSB-MP483001EOB
CSB-EP483001EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 HTH-type transcriptional regulator iscR (iscR) CSB-YP483002EOB
CSB-EP483002EOB
CSB-BP483002EOB
CSB-MP483002EOB
CSB-EP483002EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 tRNA1 (Val) (adenine (37)-N6)-methyltransferase CSB-YP483003EOB
CSB-EP483003EOB
CSB-BP483003EOB
CSB-MP483003EOB
CSB-EP483003EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 30S ribosomal protein S16 (rpsP) CSB-YP483004EOB
CSB-EP483004EOB
CSB-BP483004EOB
CSB-MP483004EOB
CSB-EP483004EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Protein nrdI (nrdI) CSB-YP483005EOB
CSB-EP483005EOB
CSB-BP483005EOB
CSB-MP483005EOB
CSB-EP483005EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Protein RecA (recA) CSB-YP483006EOB
CSB-EP483006EOB
CSB-BP483006EOB
CSB-MP483006EOB
CSB-EP483006EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP483007EOB
CSB-EP483007EOB
CSB-BP483007EOB
CSB-MP483007EOB
CSB-EP483007EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Phosphoadenosine phosphosulfate reductase (cysH) CSB-YP483008EOB
CSB-EP483008EOB
CSB-BP483008EOB
CSB-MP483008EOB
CSB-EP483008EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 L-fucose isomerase (fucI) CSB-YP483009EOB
CSB-EP483009EOB
CSB-BP483009EOB
CSB-MP483009EOB
CSB-EP483009EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Lysophospholipid transporter lplT (lplT), partial CSB-YP483010EOB
CSB-EP483010EOB
CSB-BP483010EOB
CSB-MP483010EOB
CSB-EP483010EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 tRNA-modifying protein ygfZ (ygfZ) CSB-YP483011EOB
CSB-EP483011EOB
CSB-BP483011EOB
CSB-MP483011EOB
CSB-EP483011EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 S-adenosylmethionine synthase (metK) CSB-YP483012EOB
CSB-EP483012EOB
CSB-BP483012EOB
CSB-MP483012EOB
CSB-EP483012EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Membrane-bound lytic murein transglycosylase C (mltC) CSB-YP483013EOB
CSB-EP483013EOB
CSB-BP483013EOB
CSB-MP483013EOB
CSB-EP483013EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Lipoprotein signal peptidase (lspA), partial CSB-YP483014EOB1
CSB-EP483014EOB1
CSB-BP483014EOB1
CSB-MP483014EOB1
CSB-EP483014EOB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Crotonobetainyl-CoA:carnitine CoA-transferase (caiB) CSB-YP483015EOB
CSB-EP483015EOB
CSB-BP483015EOB
CSB-MP483015EOB
CSB-EP483015EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Bis (5'-nucleosyl)-tetraphosphatase, symmetrical CSB-YP483016EOB
CSB-EP483016EOB
CSB-BP483016EOB
CSB-MP483016EOB
CSB-EP483016EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP483017EOB
CSB-EP483017EOB
CSB-BP483017EOB
CSB-MP483017EOB
CSB-EP483017EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP483018EOB
CSB-EP483018EOB
CSB-BP483018EOB
CSB-MP483018EOB
CSB-EP483018EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 GMP reductase (guaC) CSB-YP483019EOB
CSB-EP483019EOB
CSB-BP483019EOB
CSB-MP483019EOB
CSB-EP483019EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Sugar fermentation stimulation protein A (sfsA) CSB-YP483020EOB
CSB-EP483020EOB
CSB-BP483020EOB
CSB-MP483020EOB
CSB-EP483020EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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