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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Ixodes scapularis Probable cytosolic iron-sulfur protein assembly protein (ISCW023049) CSB-YP483841INM
CSB-EP483841INM
CSB-BP483841INM
CSB-MP483841INM
CSB-EP483841INM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaucheria litorea ATP synthase subunit delta, chloroplastic (atpD) CSB-YP483842VEN
CSB-EP483842VEN
CSB-BP483842VEN
CSB-MP483842VEN
CSB-EP483842VEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agkistrodon piscivorus leucostoma Zinc metalloprotease VMP1 CSB-YP483843AYO
CSB-EP483843AYO
CSB-BP483843AYO
CSB-MP483843AYO
CSB-EP483843AYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Hydroxyethylthiazole kinase (thiM) CSB-YP483844EOB
CSB-EP483844EOB
CSB-BP483844EOB
CSB-MP483844EOB
CSB-EP483844EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Probable endonuclease 4 (nfo) CSB-YP483845EOB
CSB-EP483845EOB
CSB-BP483845EOB
CSB-MP483845EOB
CSB-EP483845EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Periplasmic nitrate reductase (napA) CSB-YP483846EOB
CSB-EP483846EOB
CSB-BP483846EOB
CSB-MP483846EOB
CSB-EP483846EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB) CSB-YP483847EOB
CSB-EP483847EOB
CSB-BP483847EOB
CSB-MP483847EOB
CSB-EP483847EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnF (arnF), partial CSB-YP483848EOB1
CSB-EP483848EOB1
CSB-BP483848EOB1
CSB-MP483848EOB1
CSB-EP483848EOB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP483849EOB
CSB-EP483849EOB
CSB-BP483849EOB
CSB-MP483849EOB
CSB-EP483849EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Protein CysZ (cysZ), partial CSB-YP483850EOB1
CSB-EP483850EOB1
CSB-BP483850EOB1
CSB-MP483850EOB1
CSB-EP483850EOB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 UPF0370 protein ypfN (ypfN) CSB-YP483851EOB
CSB-EP483851EOB
CSB-BP483851EOB
CSB-MP483851EOB
CSB-EP483851EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP483852EOB
CSB-EP483852EOB
CSB-BP483852EOB
CSB-MP483852EOB
CSB-EP483852EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Autonomous glycyl radical cofactor (grcA) CSB-YP483853EOB
CSB-EP483853EOB
CSB-BP483853EOB
CSB-MP483853EOB
CSB-EP483853EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Protein grpE (grpE) CSB-YP483854EOB
CSB-EP483854EOB
CSB-BP483854EOB
CSB-MP483854EOB
CSB-EP483854EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Glutamate--cysteine ligase (gshA) CSB-YP483855EOB
CSB-EP483855EOB
CSB-BP483855EOB
CSB-MP483855EOB
CSB-EP483855EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 5'/3'-nucleotidase SurE (surE) CSB-YP483856EOB
CSB-EP483856EOB
CSB-BP483856EOB
CSB-MP483856EOB
CSB-EP483856EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Sulfate adenylyltransferase subunit 2 (cysD) CSB-YP483857EOB
CSB-EP483857EOB
CSB-BP483857EOB
CSB-MP483857EOB
CSB-EP483857EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Protein Xni (xni) CSB-YP483858EOB
CSB-EP483858EOB
CSB-BP483858EOB
CSB-MP483858EOB
CSB-EP483858EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 DNA mismatch repair protein mutH (mutH) CSB-YP483859EOB
CSB-EP483859EOB
CSB-BP483859EOB
CSB-MP483859EOB
CSB-EP483859EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Isopentenyl-diphosphate Delta-isomerase (idi) CSB-YP483860EOB
CSB-EP483860EOB
CSB-BP483860EOB
CSB-MP483860EOB
CSB-EP483860EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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