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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli O127:H6 Ribose-5-phosphate isomerase A (rpiA) CSB-YP483861EOB
CSB-EP483861EOB
CSB-BP483861EOB
CSB-MP483861EOB
CSB-EP483861EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 UPF0301 protein yqgE (yqgE) CSB-YP483862EOB
CSB-EP483862EOB
CSB-BP483862EOB
CSB-MP483862EOB
CSB-EP483862EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 UPF0246 protein yaaA (yaaA) CSB-YP483863EOB
CSB-EP483863EOB
CSB-BP483863EOB
CSB-MP483863EOB
CSB-EP483863EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP483864EOB
CSB-EP483864EOB
CSB-BP483864EOB
CSB-MP483864EOB
CSB-EP483864EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 L-carnitine/gamma-butyrobetaine antiporter (caiT), partial CSB-YP483865EOB
CSB-EP483865EOB
CSB-BP483865EOB
CSB-MP483865EOB
CSB-EP483865EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP483866EOB
CSB-EP483866EOB
CSB-BP483866EOB
CSB-MP483866EOB
CSB-EP483866EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Protein MraZ (mraZ) CSB-YP483867EOB
CSB-EP483867EOB
CSB-BP483867EOB
CSB-MP483867EOB
CSB-EP483867EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Protein translocase subunit SecA (secA), partial CSB-YP483868EOB
CSB-EP483868EOB
CSB-BP483868EOB
CSB-MP483868EOB
CSB-EP483868EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Pantothenate synthetase (panC) CSB-YP483869EOB
CSB-EP483869EOB
CSB-BP483869EOB
CSB-MP483869EOB
CSB-EP483869EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Deoxyguanosinetriphosphate triphosphohydrolase (dgt) CSB-YP483870EOB
CSB-EP483870EOB
CSB-BP483870EOB
CSB-MP483870EOB
CSB-EP483870EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Bifunctional protein HldE (hldE) CSB-YP483871EOB
CSB-EP483871EOB
CSB-BP483871EOB
CSB-MP483871EOB
CSB-EP483871EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Putrescine aminotransferase (patA) CSB-YP483872EOB
CSB-EP483872EOB
CSB-BP483872EOB
CSB-MP483872EOB
CSB-EP483872EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 DnaA initiator-associating protein diaA (diaA) CSB-YP483873EOB
CSB-EP483873EOB
CSB-BP483873EOB
CSB-MP483873EOB
CSB-EP483873EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP483874EOB
CSB-EP483874EOB
CSB-BP483874EOB
CSB-MP483874EOB
CSB-EP483874EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Proline--tRNA ligase (proS) CSB-YP483875EOB
CSB-EP483875EOB
CSB-BP483875EOB
CSB-MP483875EOB
CSB-EP483875EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Sigma factor-binding protein crl (crl) CSB-YP483876EOB
CSB-EP483876EOB
CSB-BP483876EOB
CSB-MP483876EOB
CSB-EP483876EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP483877EOB
CSB-EP483877EOB
CSB-BP483877EOB
CSB-MP483877EOB
CSB-EP483877EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP483878EOB
CSB-EP483878EOB
CSB-BP483878EOB
CSB-MP483878EOB
CSB-EP483878EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Putative sialic acid transporter (nanT), partial CSB-YP483879EOB
CSB-EP483879EOB
CSB-BP483879EOB
CSB-MP483879EOB
CSB-EP483879EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Arginine repressor (argR) CSB-YP483880EOB
CSB-EP483880EOB
CSB-BP483880EOB
CSB-MP483880EOB
CSB-EP483880EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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