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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ0047 (MJ0047) CSB-YP720127MRU
CSB-EP720127MRU
CSB-BP720127MRU
CSB-MP720127MRU
CSB-EP720127MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ0065 (MJ0065) CSB-YP720128MRU
CSB-EP720128MRU
CSB-BP720128MRU
CSB-MP720128MRU
CSB-EP720128MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ0073 (MJ0073) CSB-YP720129MRU
CSB-EP720129MRU
CSB-BP720129MRU
CSB-MP720129MRU
CSB-EP720129MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Methyl-coenzyme M reductase II subunit alpha (mrtA), partial CSB-YP720130MRU
CSB-EP720130MRU
CSB-BP720130MRU
CSB-MP720130MRU
CSB-EP720130MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylococcus capsulatus Nucleoside diphosphate kinase (ndk) CSB-YP720131MFM
CSB-EP720131MFM
CSB-BP720131MFM
CSB-MP720131MFM
CSB-EP720131MFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylococcus capsulatus Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP720132MFM
CSB-EP720132MFM
CSB-BP720132MFM
CSB-MP720132MFM
CSB-EP720132MFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylococcus capsulatus Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP720133MFM
CSB-EP720133MFM
CSB-BP720133MFM
CSB-MP720133MFM
CSB-EP720133MFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylococcus capsulatus Shikimate dehydrogenase (aroE) CSB-YP720134MFM
CSB-EP720134MFM
CSB-BP720134MFM
CSB-MP720134MFM
CSB-EP720134MFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cricetulus griseus Hypoxia up-regulated protein 1 (HYOU1), partial CSB-YP720135DXU
CSB-EP720135DXU
CSB-BP720135DXU
CSB-MP720135DXU
CSB-EP720135DXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cricetulus griseus Cysteine-rich with EGF-like domain protein 2 (CRELD2) CSB-YP720136DXU
CSB-EP720136DXU
CSB-BP720136DXU
CSB-MP720136DXU
CSB-EP720136DXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cricetulus griseus TFIIH basal transcription factor complex helicase XPD subunit (ERCC2), partial CSB-YP720137DXU
CSB-EP720137DXU
CSB-BP720137DXU
CSB-MP720137DXU
CSB-EP720137DXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Guinea pig Disintegrin and metalloproteinase domain-containing protein 5 (ADAM5), partial CSB-YP720138GU
CSB-EP720138GU
CSB-BP720138GU
CSB-MP720138GU
CSB-EP720138GU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Guinea pig Proenkephalin-B (PDYN) CSB-YP720139GU
CSB-EP720139GU
CSB-BP720139GU
CSB-MP720139GU
CSB-EP720139GU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylococcus capsulatus Probable GTP-binding protein EngB (engB) CSB-YP720140MFM
CSB-EP720140MFM
CSB-BP720140MFM
CSB-MP720140MFM
CSB-EP720140MFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylococcus capsulatus Ribonuclease HII (rnhB) CSB-YP720141MFM
CSB-EP720141MFM
CSB-BP720141MFM
CSB-MP720141MFM
CSB-EP720141MFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylococcus capsulatus Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP720142MFM
CSB-EP720142MFM
CSB-BP720142MFM
CSB-MP720142MFM
CSB-EP720142MFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylococcus capsulatus UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP720143MFM
CSB-EP720143MFM
CSB-BP720143MFM
CSB-MP720143MFM
CSB-EP720143MFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylococcus capsulatus 5'-nucleotidase surE (surE) CSB-YP720144MFM
CSB-EP720144MFM
CSB-BP720144MFM
CSB-MP720144MFM
CSB-EP720144MFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus E3 ubiquitin-protein ligase Mdm2 (MDM2) CSB-YP720145MRG
CSB-EP720145MRG
CSB-BP720145MRG
CSB-MP720145MRG
CSB-EP720145MRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus Corticosteroid-binding globulin (SERPINA6) CSB-YP720146MRG
CSB-EP720146MRG
CSB-BP720146MRG
CSB-MP720146MRG
CSB-EP720146MRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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