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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Desulfotalea psychrophila Uridylate kinase (pyrH) CSB-YP721427DAAA
CSB-EP721427DAAA
CSB-BP721427DAAA
CSB-MP721427DAAA
CSB-EP721427DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila 50S ribosomal protein L36 (rpmJ) CSB-YP721428DAAA
CSB-EP721428DAAA
CSB-BP721428DAAA
CSB-MP721428DAAA
CSB-EP721428DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila 50S ribosomal protein L24 (rplX) CSB-YP721429DAAA
CSB-EP721429DAAA
CSB-BP721429DAAA
CSB-MP721429DAAA
CSB-EP721429DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila 50S ribosomal protein L23 (rplW) CSB-YP721430DAAA
CSB-EP721430DAAA
CSB-BP721430DAAA
CSB-MP721430DAAA
CSB-EP721430DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila 30S ribosomal protein S12 (rpsL) CSB-YP721431DAAA
CSB-EP721431DAAA
CSB-BP721431DAAA
CSB-MP721431DAAA
CSB-EP721431DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila N utilization substance protein B homolog (nusB) CSB-YP721432DAAA
CSB-EP721432DAAA
CSB-BP721432DAAA
CSB-MP721432DAAA
CSB-EP721432DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila UPF0059 membrane protein DP0890 (DP0890), partial CSB-YP721433DAAA1
CSB-EP721433DAAA1
CSB-BP721433DAAA1
CSB-MP721433DAAA1
CSB-EP721433DAAA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila ATP synthase subunit beta (atpD) CSB-YP721434DAAA
CSB-EP721434DAAA
CSB-BP721434DAAA
CSB-MP721434DAAA
CSB-EP721434DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila ATP synthase subunit c (atpE), partial CSB-YP721435DAAA1
CSB-EP721435DAAA1
CSB-BP721435DAAA1
CSB-MP721435DAAA1
CSB-EP721435DAAA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Methionyl-tRNA formyltransferase (fmt) CSB-YP721436DAAA
CSB-EP721436DAAA
CSB-BP721436DAAA
CSB-MP721436DAAA
CSB-EP721436DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Xanthine phosphoribosyltransferase (gpt) CSB-YP721437DAAA
CSB-EP721437DAAA
CSB-BP721437DAAA
CSB-MP721437DAAA
CSB-EP721437DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila UPF0597 protein DP0591 (DP0591) CSB-YP721438DAAA
CSB-EP721438DAAA
CSB-BP721438DAAA
CSB-MP721438DAAA
CSB-EP721438DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila SsrA-binding protein (smpB) CSB-YP721439DAAA
CSB-EP721439DAAA
CSB-BP721439DAAA
CSB-MP721439DAAA
CSB-EP721439DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Probable transaldolase (tal) CSB-YP721440DAAA
CSB-EP721440DAAA
CSB-BP721440DAAA
CSB-MP721440DAAA
CSB-EP721440DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Chaperone protein htpG (htpG), partial CSB-YP721441DAAA
CSB-EP721441DAAA
CSB-BP721441DAAA
CSB-MP721441DAAA
CSB-EP721441DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila 7-cyano-7-deazaguanine synthase (queC) CSB-YP721442DAAA
CSB-EP721442DAAA
CSB-BP721442DAAA
CSB-MP721442DAAA
CSB-EP721442DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Dihydroorotase (pyrC) CSB-YP721443DAAA
CSB-EP721443DAAA
CSB-BP721443DAAA
CSB-MP721443DAAA
CSB-EP721443DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP721444BUI
CSB-EP721444BUI
CSB-BP721444BUI
CSB-MP721444BUI
CSB-EP721444BUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable glucuronosyltransferase Os05g0559600 (Os05g0559600, LOC_Os05g48600), partial CSB-YP721445OFG
CSB-EP721445OFG
CSB-BP721445OFG
CSB-MP721445OFG
CSB-EP721445OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Sulfhydryl oxidase 1 (QSOX1) CSB-YP721446OFG
CSB-EP721446OFG
CSB-BP721446OFG
CSB-MP721446OFG
CSB-EP721446OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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