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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pongo abelii AMSH-like protease (STAMBPL1) CSB-YP722269PYX
CSB-EP722269PYX
CSB-BP722269PYX
CSB-MP722269PYX
CSB-EP722269PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii MORN repeat-containing protein 4 (MORN4) CSB-YP722270PYX
CSB-EP722270PYX
CSB-BP722270PYX
CSB-MP722270PYX
CSB-EP722270PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Deoxynucleotidyltransferase terminal-interacting protein 1 (DNTTIP1) CSB-YP722271PYX
CSB-EP722271PYX
CSB-BP722271PYX
CSB-MP722271PYX
CSB-EP722271PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Cyclin-G1 (CCNG1) CSB-YP722272PYX
CSB-EP722272PYX
CSB-BP722272PYX
CSB-MP722272PYX
CSB-EP722272PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Probable histidine--tRNA ligase, mitochondrial (HARS2) CSB-YP722273PYX
CSB-EP722273PYX
CSB-BP722273PYX
CSB-MP722273PYX
CSB-EP722273PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Protein transport protein Sec23B (SEC23B), partial CSB-YP722274PYX
CSB-EP722274PYX
CSB-BP722274PYX
CSB-MP722274PYX
CSB-EP722274PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Ubiquitin-conjugating enzyme E2 L3 (UBE2L3) CSB-YP722275PYX
CSB-EP722275PYX
CSB-BP722275PYX
CSB-MP722275PYX
CSB-EP722275PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Gamma-tubulin complex component 2 (TUBGCP2), partial CSB-YP722276PYX
CSB-EP722276PYX
CSB-BP722276PYX
CSB-MP722276PYX
CSB-EP722276PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Arrestin domain-containing protein 3 (ARRDC3) CSB-YP722277PYX
CSB-EP722277PYX
CSB-BP722277PYX
CSB-MP722277PYX
CSB-EP722277PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii RAF proto-oncogene serine/threonine-protein kinase (RAF1), partial CSB-YP722278PYX
CSB-EP722278PYX
CSB-BP722278PYX
CSB-MP722278PYX
CSB-EP722278PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Serine/arginine repetitive matrix protein 1 (SRRM1), partial CSB-YP722279PYX
CSB-EP722279PYX
CSB-BP722279PYX
CSB-MP722279PYX
CSB-EP722279PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Cellular nucleic acid-binding protein (CNBP) CSB-YP722280PYX
CSB-EP722280PYX
CSB-BP722280PYX
CSB-MP722280PYX
CSB-EP722280PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Probable methyltransferase-like protein 15 (METTL15) CSB-YP722281PYX
CSB-EP722281PYX
CSB-BP722281PYX
CSB-MP722281PYX
CSB-EP722281PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Vacuolar protein sorting-associated protein 72 homolog (VPS72) CSB-YP722282PYX
CSB-EP722282PYX
CSB-BP722282PYX
CSB-MP722282PYX
CSB-EP722282PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Voltage-dependent calcium channel gamma-3 subunit (CACNG3), partial CSB-YP722283PYX1
CSB-EP722283PYX1
CSB-BP722283PYX1
CSB-MP722283PYX1
CSB-EP722283PYX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Nuclear receptor subfamily 4 group A member 2 (NR4A2), partial CSB-YP722284PYX
CSB-EP722284PYX
CSB-BP722284PYX
CSB-MP722284PYX
CSB-EP722284PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Vesicle-associated membrane protein-associated protein A (VAPA), partial CSB-YP722285PYX
CSB-EP722285PYX
CSB-BP722285PYX
CSB-MP722285PYX
CSB-EP722285PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Leucine--tRNA ligase, cytoplasmic (LARS), partial CSB-YP722286PYX
CSB-EP722286PYX
CSB-BP722286PYX
CSB-MP722286PYX
CSB-EP722286PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Zinc transporter 3 (SLC30A3), partial CSB-YP722287PYX1
CSB-EP722287PYX1
CSB-BP722287PYX1
CSB-MP722287PYX1
CSB-EP722287PYX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Adaptin ear-binding coat-associated protein 1 (NECAP1) CSB-YP722288PYX
CSB-EP722288PYX
CSB-BP722288PYX
CSB-MP722288PYX
CSB-EP722288PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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