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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia pseudomallei Adenylosuccinate synthetase (purA) CSB-YP723690BXW
CSB-EP723690BXW
CSB-BP723690BXW
CSB-MP723690BXW
CSB-EP723690BXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP723691BXW
CSB-EP723691BXW
CSB-BP723691BXW
CSB-MP723691BXW
CSB-EP723691BXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 30S ribosomal protein S18 (rpsR) CSB-YP723692BXW
CSB-EP723692BXW
CSB-BP723692BXW
CSB-MP723692BXW
CSB-EP723692BXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei UPF0061 protein BPSL1422 (BPSL1422), partial CSB-YP723693BXW
CSB-EP723693BXW
CSB-BP723693BXW
CSB-MP723693BXW
CSB-EP723693BXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei UPF0060 membrane protein BPSL1340 (BPSL1340), partial CSB-YP723694BXW1
CSB-EP723694BXW1
CSB-BP723694BXW1
CSB-MP723694BXW1
CSB-EP723694BXW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei NADH-quinone oxidoreductase subunit C (nuoC) CSB-YP723695BXW
CSB-EP723695BXW
CSB-BP723695BXW
CSB-MP723695BXW
CSB-EP723695BXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP723696BXW
CSB-EP723696BXW
CSB-BP723696BXW
CSB-MP723696BXW
CSB-EP723696BXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Porphobilinogen deaminase (hemC) CSB-YP723697BXW
CSB-EP723697BXW
CSB-BP723697BXW
CSB-MP723697BXW
CSB-EP723697BXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP723698BXW1
CSB-EP723698BXW1
CSB-BP723698BXW1
CSB-MP723698BXW1
CSB-EP723698BXW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 50S ribosomal protein L33 (rpmG) CSB-YP723699BXW
CSB-EP723699BXW
CSB-BP723699BXW
CSB-MP723699BXW
CSB-EP723699BXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 30S ribosomal protein S20 (rpsT) CSB-YP723700BXW
CSB-EP723700BXW
CSB-BP723700BXW
CSB-MP723700BXW
CSB-EP723700BXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei LPS-assembly protein lptD (lptD), partial CSB-YP723701BXW
CSB-EP723701BXW
CSB-BP723701BXW
CSB-MP723701BXW
CSB-EP723701BXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP723702BXW
CSB-EP723702BXW
CSB-BP723702BXW
CSB-MP723702BXW
CSB-EP723702BXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Protein-export protein SecB (secB) CSB-YP723703BXW
CSB-EP723703BXW
CSB-BP723703BXW
CSB-MP723703BXW
CSB-EP723703BXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Isocitrate dehydrogenase kinase/phosphatase (aceK), partial CSB-YP723704BXW
CSB-EP723704BXW
CSB-BP723704BXW
CSB-MP723704BXW
CSB-EP723704BXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Flagellar P-ring protein (flgI) CSB-YP723705BXW
CSB-EP723705BXW
CSB-BP723705BXW
CSB-MP723705BXW
CSB-EP723705BXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Glutamyl-tRNA (Gln) amidotransferase subunit A (gatA) CSB-YP723706BXW
CSB-EP723706BXW
CSB-BP723706BXW
CSB-MP723706BXW
CSB-EP723706BXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Membrane protein insertase YidC (yidC), partial CSB-YP723707BXW1
CSB-EP723707BXW1
CSB-BP723707BXW1
CSB-MP723707BXW1
CSB-EP723707BXW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Solute carrier family 22 member 10 (SLC22A10), partial CSB-YP723708HU
CSB-EP723708HU
CSB-BP723708HU
CSB-MP723708HU
CSB-EP723708HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Heme transporter hrg1-A (slc48a1-a), partial CSB-YP723709XBE1
CSB-EP723709XBE1
CSB-BP723709XBE1
CSB-MP723709XBE1
CSB-EP723709XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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