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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pongo abelii Rho guanine nucleotide exchange factor 3 (ARHGEF3), partial CSB-YP725293PYX
CSB-EP725293PYX
CSB-BP725293PYX
CSB-MP725293PYX
CSB-EP725293PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii mTERF domain-containing protein 3, mitochondrial (MTERFD3) CSB-YP725294PYX
CSB-EP725294PYX
CSB-BP725294PYX
CSB-MP725294PYX
CSB-EP725294PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii DnaJ homolog subfamily B member 14 (DNAJB14), partial CSB-YP725295PYX1
CSB-EP725295PYX1
CSB-BP725295PYX1
CSB-MP725295PYX1
CSB-EP725295PYX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Proteasome-associated protein ECM29 homolog (ECM29), partial CSB-YP725296PYX
CSB-EP725296PYX
CSB-BP725296PYX
CSB-MP725296PYX
CSB-EP725296PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Procollagen-lysine,2-oxoglutarate 5-dioxygenase 3 (PLOD3), partial CSB-YP725297PYX
CSB-EP725297PYX
CSB-BP725297PYX
CSB-MP725297PYX
CSB-EP725297PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii PNMA-like protein 1 (PNMAL1) CSB-YP725298PYX
CSB-EP725298PYX
CSB-BP725298PYX
CSB-MP725298PYX
CSB-EP725298PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Probable RNA-binding protein 18 (RBM18) CSB-YP725299PYX
CSB-EP725299PYX
CSB-BP725299PYX
CSB-MP725299PYX
CSB-EP725299PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Origin recognition complex subunit 4 (ORC4) CSB-YP725300PYX
CSB-EP725300PYX
CSB-BP725300PYX
CSB-MP725300PYX
CSB-EP725300PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Cytoplasmic polyadenylation element-binding protein 1 (CPEB1) CSB-YP725301PYX
CSB-EP725301PYX
CSB-BP725301PYX
CSB-MP725301PYX
CSB-EP725301PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii U4/U6.U5 tri-snRNP-associated protein 2 (USP39), partial CSB-YP725302PYX
CSB-EP725302PYX
CSB-BP725302PYX
CSB-MP725302PYX
CSB-EP725302PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Acyl-CoA dehydrogenase family member 11 (ACAD11), partial CSB-YP725303PYX
CSB-EP725303PYX
CSB-BP725303PYX
CSB-MP725303PYX
CSB-EP725303PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Torsin-1A-interacting protein 1 (TOR1AIP1), partial CSB-YP725304PYX1
CSB-EP725304PYX1
CSB-BP725304PYX1
CSB-MP725304PYX1
CSB-EP725304PYX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Creatine kinase S-type, mitochondrial (CKMT2) CSB-YP725305PYX
CSB-EP725305PYX
CSB-BP725305PYX
CSB-MP725305PYX
CSB-EP725305PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Mitochondrial inner membrane protein COX18 (COX18), partial CSB-YP725306PYX
CSB-EP725306PYX
CSB-BP725306PYX
CSB-MP725306PYX
CSB-EP725306PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Multidrug and toxin extrusion protein 2 (SLC47A2), partial CSB-YP725307PYX
CSB-EP725307PYX
CSB-BP725307PYX
CSB-MP725307PYX
CSB-EP725307PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Calcium-binding protein p22 (CHP) CSB-YP725308PYX
CSB-EP725308PYX
CSB-BP725308PYX
CSB-MP725308PYX
CSB-EP725308PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Cytoskeleton-associated protein 2 (CKAP2), partial CSB-YP725309PYX
CSB-EP725309PYX
CSB-BP725309PYX
CSB-MP725309PYX
CSB-EP725309PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Thymosin beta-4 (TMSB4X) CSB-YP725310PYX
CSB-EP725310PYX
CSB-BP725310PYX
CSB-MP725310PYX
CSB-EP725310PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Protein tyrosine phosphatase type IVA 1 (PTP4A1) CSB-YP725311PYX
CSB-EP725311PYX
CSB-BP725311PYX
CSB-MP725311PYX
CSB-EP725311PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii 39S ribosomal protein L16, mitochondrial (MRPL16) CSB-YP725312PYX
CSB-EP725312PYX
CSB-BP725312PYX
CSB-MP725312PYX
CSB-EP725312PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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