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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mannheimia succiniciproducens Homoserine kinase (thrB) CSB-YP723929MAAB
CSB-EP723929MAAB
CSB-BP723929MAAB
CSB-MP723929MAAB
CSB-EP723929MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP723930MAAB
CSB-EP723930MAAB
CSB-BP723930MAAB
CSB-MP723930MAAB
CSB-EP723930MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Uncharacterized protein YjeA (yjeA) CSB-YP723931MAAB
CSB-EP723931MAAB
CSB-BP723931MAAB
CSB-MP723931MAAB
CSB-EP723931MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC), partial CSB-YP723932MAAB
CSB-EP723932MAAB
CSB-BP723932MAAB
CSB-MP723932MAAB
CSB-EP723932MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP723933MAAB
CSB-EP723933MAAB
CSB-BP723933MAAB
CSB-MP723933MAAB
CSB-EP723933MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN) CSB-YP723934MAAB
CSB-EP723934MAAB
CSB-BP723934MAAB
CSB-MP723934MAAB
CSB-EP723934MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens N-acetyl-D-glucosamine kinase (nagK) CSB-YP723935MAAB
CSB-EP723935MAAB
CSB-BP723935MAAB
CSB-MP723935MAAB
CSB-EP723935MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Protein hfq (hfq) CSB-YP723936MAAB
CSB-EP723936MAAB
CSB-BP723936MAAB
CSB-MP723936MAAB
CSB-EP723936MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Cytidylate kinase (cmk) CSB-YP723937MAAB
CSB-EP723937MAAB
CSB-BP723937MAAB
CSB-MP723937MAAB
CSB-EP723937MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens UPF0756 membrane protein MS1439 (MS1439), partial CSB-YP723938MAAB1
CSB-EP723938MAAB1
CSB-BP723938MAAB1
CSB-MP723938MAAB1
CSB-EP723938MAAB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP723939MAAB
CSB-EP723939MAAB
CSB-BP723939MAAB
CSB-MP723939MAAB
CSB-EP723939MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens [Protein-PII] uridylyltransferase (glnD), partial CSB-YP723940MAAB
CSB-EP723940MAAB
CSB-BP723940MAAB
CSB-MP723940MAAB
CSB-EP723940MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Tyrosine--tRNA ligase (tyrS) CSB-YP723941MAAB
CSB-EP723941MAAB
CSB-BP723941MAAB
CSB-MP723941MAAB
CSB-EP723941MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP723942MAAB
CSB-EP723942MAAB
CSB-BP723942MAAB
CSB-MP723942MAAB
CSB-EP723942MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Tryptophan synthase beta chain (trpB) CSB-YP723943MAAB
CSB-EP723943MAAB
CSB-BP723943MAAB
CSB-MP723943MAAB
CSB-EP723943MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens 50S ribosomal protein L25 (rplY) CSB-YP723944MAAB
CSB-EP723944MAAB
CSB-BP723944MAAB
CSB-MP723944MAAB
CSB-EP723944MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Integration host factor subunit alpha (ihfA) CSB-YP723945MAAB
CSB-EP723945MAAB
CSB-BP723945MAAB
CSB-MP723945MAAB
CSB-EP723945MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens UPF0181 protein MS1074 (MS1074) CSB-YP723946MAAB
CSB-EP723946MAAB
CSB-BP723946MAAB
CSB-MP723946MAAB
CSB-EP723946MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens 50S ribosomal protein L20 (rplT) CSB-YP723947MAAB
CSB-EP723947MAAB
CSB-BP723947MAAB
CSB-MP723947MAAB
CSB-EP723947MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens UvrABC system protein C (uvrC), partial CSB-YP723948MAAB
CSB-EP723948MAAB
CSB-BP723948MAAB
CSB-MP723948MAAB
CSB-EP723948MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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