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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus licheniformis Thymidylate kinase (tmk) CSB-YP723909BQU
CSB-EP723909BQU
CSB-BP723909BQU
CSB-MP723909BQU
CSB-EP723909BQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus licheniformis DNA replication and repair protein recF (recF) CSB-YP723910BQU
CSB-EP723910BQU
CSB-BP723910BQU
CSB-MP723910BQU
CSB-EP723910BQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP723911MAAB
CSB-EP723911MAAB
CSB-BP723911MAAB
CSB-MP723911MAAB
CSB-EP723911MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens ATP synthase subunit b (atpF), partial CSB-YP723912MAAB
CSB-EP723912MAAB
CSB-BP723912MAAB
CSB-MP723912MAAB
CSB-EP723912MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Rhamnulokinase (rhaB) CSB-YP723913MAAB
CSB-EP723913MAAB
CSB-BP723913MAAB
CSB-MP723913MAAB
CSB-EP723913MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Cell division protein FtsB (ftsB), partial CSB-YP723914MAAB
CSB-EP723914MAAB
CSB-BP723914MAAB
CSB-MP723914MAAB
CSB-EP723914MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Cell division protein ZapB (zapB) CSB-YP723915MAAB
CSB-EP723915MAAB
CSB-BP723915MAAB
CSB-MP723915MAAB
CSB-EP723915MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Glycerol-3-phosphate dehydrogenase [NAD (P)+] (gpsA) CSB-YP723916MAAB
CSB-EP723916MAAB
CSB-BP723916MAAB
CSB-MP723916MAAB
CSB-EP723916MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens 50S ribosomal protein L3 (rplC) CSB-YP723917MAAB
CSB-EP723917MAAB
CSB-BP723917MAAB
CSB-MP723917MAAB
CSB-EP723917MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens 50S ribosomal protein L22 (rplV) CSB-YP723918MAAB
CSB-EP723918MAAB
CSB-BP723918MAAB
CSB-MP723918MAAB
CSB-EP723918MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens 30S ribosomal protein S17 (rpsQ) CSB-YP723919MAAB
CSB-EP723919MAAB
CSB-BP723919MAAB
CSB-MP723919MAAB
CSB-EP723919MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens 50S ribosomal protein L6 (rplF) CSB-YP723920MAAB
CSB-EP723920MAAB
CSB-BP723920MAAB
CSB-MP723920MAAB
CSB-EP723920MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens 30S ribosomal protein S13 (rpsM) CSB-YP723921MAAB
CSB-EP723921MAAB
CSB-BP723921MAAB
CSB-MP723921MAAB
CSB-EP723921MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP723922MAAB
CSB-EP723922MAAB
CSB-BP723922MAAB
CSB-MP723922MAAB
CSB-EP723922MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Uridylate kinase (pyrH) CSB-YP723923MAAB
CSB-EP723923MAAB
CSB-BP723923MAAB
CSB-MP723923MAAB
CSB-EP723923MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Imidazole glycerol phosphate synthase subunit HisH (hisH) CSB-YP723924MAAB
CSB-EP723924MAAB
CSB-BP723924MAAB
CSB-MP723924MAAB
CSB-EP723924MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Uracil phosphoribosyltransferase (upp) CSB-YP723925MAAB
CSB-EP723925MAAB
CSB-BP723925MAAB
CSB-MP723925MAAB
CSB-EP723925MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Trigger factor (tig) CSB-YP723926MAAB
CSB-EP723926MAAB
CSB-BP723926MAAB
CSB-MP723926MAAB
CSB-EP723926MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP723927MAAB
CSB-EP723927MAAB
CSB-BP723927MAAB
CSB-MP723927MAAB
CSB-EP723927MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens UPF0234 protein MS1759 (MS1759) CSB-YP723928MAAB
CSB-EP723928MAAB
CSB-BP723928MAAB
CSB-MP723928MAAB
CSB-EP723928MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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