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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Debaryomyces hansenii Amino-acid acetyltransferase, mitochondrial (ARG2), partial CSB-YP724631DIS
CSB-EP724631DIS
CSB-BP724631DIS
CSB-MP724631DIS
CSB-EP724631DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Mediator of RNA polymerase II transcription subunit 13 (SSN2), partial CSB-YP724632DIS
CSB-EP724632DIS
CSB-BP724632DIS
CSB-MP724632DIS
CSB-EP724632DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Protein transport protein BOS1 (BOS1), partial CSB-YP724633YAP
CSB-EP724633YAP
CSB-BP724633YAP
CSB-MP724633YAP
CSB-EP724633YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica RNA polymerase II transcription factor B subunit 2 (TFB2) CSB-YP724634YAP
CSB-EP724634YAP
CSB-BP724634YAP
CSB-MP724634YAP
CSB-EP724634YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica DNA polymerase epsilon subunit B (DPB2), partial CSB-YP724635YAP
CSB-EP724635YAP
CSB-BP724635YAP
CSB-MP724635YAP
CSB-EP724635YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Ubiquitin-conjugating enzyme E2 2 (UBC2) CSB-YP724636YAP
CSB-EP724636YAP
CSB-BP724636YAP
CSB-MP724636YAP
CSB-EP724636YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica 13 kDa ribonucleoprotein-associated protein (SNU13) CSB-YP724637YAP
CSB-EP724637YAP
CSB-BP724637YAP
CSB-MP724637YAP
CSB-EP724637YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica tRNA (adenine (58)-N (1))-methyltransferase catalytic subunit TRM61 (TRM61) CSB-YP724638YAP
CSB-EP724638YAP
CSB-BP724638YAP
CSB-MP724638YAP
CSB-EP724638YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica ATP-dependent RNA helicase DHH1 (DHH1), partial CSB-YP724639YAP
CSB-EP724639YAP
CSB-BP724639YAP
CSB-MP724639YAP
CSB-EP724639YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Eukaryotic translation initiation factor 3 subunit A (TIF32), partial CSB-YP724640YAP
CSB-EP724640YAP
CSB-BP724640YAP
CSB-MP724640YAP
CSB-EP724640YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica RNA polymerase II transcription factor B subunit 5 (TFB5) CSB-YP724641YAP
CSB-EP724641YAP
CSB-BP724641YAP
CSB-MP724641YAP
CSB-EP724641YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Inorganic pyrophosphatase (IPP1) CSB-YP724642YAP
CSB-EP724642YAP
CSB-BP724642YAP
CSB-MP724642YAP
CSB-EP724642YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Protein SQS1 (SQS1), partial CSB-YP724643YAP
CSB-EP724643YAP
CSB-BP724643YAP
CSB-MP724643YAP
CSB-EP724643YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Sterol 24-C-methyltransferase (ERG6) CSB-YP724644YAP
CSB-EP724644YAP
CSB-BP724644YAP
CSB-MP724644YAP
CSB-EP724644YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Putative lipase ATG15 (ATG15), partial CSB-YP724645YAP
CSB-EP724645YAP
CSB-BP724645YAP
CSB-MP724645YAP
CSB-EP724645YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica DASH complex subunit DAM1 (DAM1) CSB-YP724646YAP
CSB-EP724646YAP
CSB-BP724646YAP
CSB-MP724646YAP
CSB-EP724646YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Autophagy-related protein 13 (ATG13), partial CSB-YP724647YAP
CSB-EP724647YAP
CSB-BP724647YAP
CSB-MP724647YAP
CSB-EP724647YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Chitobiosyldiphosphodolichol beta-mannosyltransferase (ALG1), partial CSB-YP724648YAP1
CSB-EP724648YAP1
CSB-BP724648YAP1
CSB-MP724648YAP1
CSB-EP724648YAP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Mediator of RNA polymerase II transcription subunit 6 (MED6) CSB-YP724649YAP
CSB-EP724649YAP
CSB-BP724649YAP
CSB-MP724649YAP
CSB-EP724649YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica U2 small nuclear ribonucleoprotein A' (LEA1) CSB-YP724650YAP
CSB-EP724650YAP
CSB-BP724650YAP
CSB-MP724650YAP
CSB-EP724650YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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