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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Legionella pneumophila subsp. pneumophila UPF0176 protein lpg2838 (lpg2838) CSB-YP726551LDL
CSB-EP726551LDL
CSB-BP726551LDL
CSB-MP726551LDL
CSB-EP726551LDL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila subsp. pneumophila Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP726552LDL
CSB-EP726552LDL
CSB-BP726552LDL
CSB-MP726552LDL
CSB-EP726552LDL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila subsp. pneumophila NADH-quinone oxidoreductase subunit I (nuoI) CSB-YP726553LDL
CSB-EP726553LDL
CSB-BP726553LDL
CSB-MP726553LDL
CSB-EP726553LDL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila subsp. pneumophila Multifunctional CCA protein (cca) CSB-YP726554LDL
CSB-EP726554LDL
CSB-BP726554LDL
CSB-MP726554LDL
CSB-EP726554LDL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila subsp. pneumophila 50S ribosomal protein L13 (rplM) CSB-YP726555LDL
CSB-EP726555LDL
CSB-BP726555LDL
CSB-MP726555LDL
CSB-EP726555LDL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila subsp. pneumophila Peptidyl-tRNA hydrolase (pth) CSB-YP726556LDL
CSB-EP726556LDL
CSB-BP726556LDL
CSB-MP726556LDL
CSB-EP726556LDL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila subsp. pneumophila GTPase obg (obg) CSB-YP726557LDL
CSB-EP726557LDL
CSB-BP726557LDL
CSB-MP726557LDL
CSB-EP726557LDL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila subsp. pneumophila D-alanine--D-alanine ligase (ddl) CSB-YP726558LDL
CSB-EP726558LDL
CSB-BP726558LDL
CSB-MP726558LDL
CSB-EP726558LDL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila subsp. pneumophila Probable hydrogenase nickel incorporation protein HypA (hypA) CSB-YP726559LDL
CSB-EP726559LDL
CSB-BP726559LDL
CSB-MP726559LDL
CSB-EP726559LDL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila subsp. pneumophila Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP726560LDL1
CSB-EP726560LDL1
CSB-BP726560LDL1
CSB-MP726560LDL1
CSB-EP726560LDL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila subsp. pneumophila Protein-export protein SecB (secB) CSB-YP726561LDL
CSB-EP726561LDL
CSB-BP726561LDL
CSB-MP726561LDL
CSB-EP726561LDL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila subsp. pneumophila Probable potassium transport system protein kup 2 (kup2), partial CSB-YP726562LDL
CSB-EP726562LDL
CSB-BP726562LDL
CSB-MP726562LDL
CSB-EP726562LDL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila subsp. pneumophila Ribonuclease PH (rph) CSB-YP726563LDL
CSB-EP726563LDL
CSB-BP726563LDL
CSB-MP726563LDL
CSB-EP726563LDL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila subsp. pneumophila Dihydroorotate dehydrogenase (quinone) (pyrD) CSB-YP726564LDL
CSB-EP726564LDL
CSB-BP726564LDL
CSB-MP726564LDL
CSB-EP726564LDL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila subsp. pneumophila Lysine--tRNA ligase (lysS) CSB-YP726565LDL
CSB-EP726565LDL
CSB-BP726565LDL
CSB-MP726565LDL
CSB-EP726565LDL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila subsp. pneumophila GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP726566LDL
CSB-EP726566LDL
CSB-BP726566LDL
CSB-MP726566LDL
CSB-EP726566LDL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila subsp. pneumophila Gamma-glutamyl phosphate reductase (proA) CSB-YP726567LDL
CSB-BP726567LDL
CSB-MP726567LDL
CSB-EP726567LDL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila subsp. pneumophila Nucleoside diphosphate kinase (ndk) CSB-YP726568LDL
CSB-EP726568LDL
CSB-BP726568LDL
CSB-MP726568LDL
CSB-EP726568LDL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila subsp. pneumophila Putative phosphotransferase lpg1528 (lpg1528) CSB-YP726569LDL
CSB-EP726569LDL
CSB-BP726569LDL
CSB-MP726569LDL
CSB-EP726569LDL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila subsp. pneumophila 4-hydroxybenzoate octaprenyltransferase (ubiA), partial CSB-YP726570LDL
CSB-EP726570LDL
CSB-BP726570LDL
CSB-MP726570LDL
CSB-EP726570LDL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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