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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Nymphaea alba NAD (P)H-quinone oxidoreductase subunit 6, chloroplastic (ndhG), partial CSB-YP725012NES1
CSB-EP725012NES1
CSB-BP725012NES1
CSB-MP725012NES1
CSB-EP725012NES1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nymphaea alba 50S ribosomal protein L22, chloroplastic (rpl22) CSB-YP725013NES
CSB-EP725013NES
CSB-BP725013NES
CSB-MP725013NES
CSB-EP725013NES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nymphaea alba DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP725014NES
CSB-EP725014NES
CSB-BP725014NES
CSB-MP725014NES
CSB-EP725014NES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nymphaea alba 50S ribosomal protein L20, chloroplastic (rpl20) CSB-YP725015NES
CSB-EP725015NES
CSB-BP725015NES
CSB-MP725015NES
CSB-EP725015NES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nymphaea alba Photosystem II reaction center protein L (psbL), partial CSB-YP725016NES
CSB-EP725016NES
CSB-BP725016NES
CSB-MP725016NES
CSB-EP725016NES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nymphaea alba NAD (P)H-quinone oxidoreductase subunit K, chloroplastic (ndhK) CSB-YP725017NES
CSB-EP725017NES
CSB-BP725017NES
CSB-MP725017NES
CSB-EP725017NES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nymphaea alba Photosystem II reaction center protein M (psbM), partial CSB-YP725018NES
CSB-EP725018NES
CSB-BP725018NES
CSB-MP725018NES
CSB-EP725018NES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nymphaea alba ATP synthase subunit a, chloroplastic (atpI), partial CSB-YP725019NES1
CSB-EP725019NES1
CSB-BP725019NES1
CSB-MP725019NES1
CSB-EP725019NES1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nymphaea alba Photosystem Q (B) protein (psbA), partial CSB-YP725020NES1
CSB-EP725020NES1
CSB-BP725020NES1
CSB-MP725020NES1
CSB-EP725020NES1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Eukaryotic translation initiation factor 5A-1 (EIF5A) CSB-YP725021BO
CSB-EP725021BO
CSB-BP725021BO
CSB-MP725021BO
CSB-EP725021BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Platanus occidentalis Protein psbN (psbN), partial CSB-YP725022PMU
CSB-EP725022PMU
CSB-BP725022PMU
CSB-MP725022PMU
CSB-EP725022PMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Houttuynia cordata Photosystem II reaction center protein T (psbT), partial CSB-YP725023HCAP
CSB-EP725023HCAP
CSB-BP725023HCAP
CSB-MP725023HCAP
CSB-EP725023HCAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aristolochia macrophylla Photosystem II reaction center protein T (psbT), partial CSB-YP725024AEAN
CSB-EP725024AEAN
CSB-BP725024AEAN
CSB-MP725024AEAN
CSB-EP725024AEAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saruma henryi Cytochrome b559 subunit beta (psbF), partial CSB-YP725025SFAI
CSB-EP725025SFAI
CSB-BP725025SFAI
CSB-MP725025SFAI
CSB-EP725025SFAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cornus mas Photosystem II reaction center protein L (psbL), partial CSB-YP725026CGAH
CSB-EP725026CGAH
CSB-BP725026CGAH
CSB-MP725026CGAH
CSB-EP725026CGAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein pXO2-37/BXB0038/GBAA_pXO2_0038 (pXO2-37, BXB0038, GBAA_pXO2_0038) CSB-YP725027BQE
CSB-EP725027BQE
CSB-BP725027BQE
CSB-MP725027BQE
CSB-EP725027BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaplasma marginale UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) CSB-YP725029AAAC
CSB-EP725029AAAC
CSB-BP725029AAAC
CSB-MP725029AAAC
CSB-EP725029AAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaplasma marginale Phosphomethylpyrimidine synthase (thiC), partial CSB-YP725030AAAC
CSB-EP725030AAAC
CSB-BP725030AAAC
CSB-MP725030AAAC
CSB-EP725030AAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaplasma marginale Zinc import ATP-binding protein ZnuC (znuC) CSB-YP725031AAAC
CSB-EP725031AAAC
CSB-BP725031AAAC
CSB-MP725031AAAC
CSB-EP725031AAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaplasma marginale DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP725032AAAC
CSB-EP725032AAAC
CSB-BP725032AAAC
CSB-MP725032AAAC
CSB-EP725032AAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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