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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus cereus Peptidase T (pepT) CSB-YP726932BAAD
CSB-EP726932BAAD
CSB-BP726932BAAD
CSB-MP726932BAAD
CSB-EP726932BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Vomeromodulin CSB-YP726933RA
CSB-EP726933RA
CSB-BP726933RA
CSB-MP726933RA
CSB-EP726933RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphonates import ATP-binding protein PhnC (phnC) CSB-YP726934BAAD
CSB-EP726934BAAD
CSB-BP726934BAAD
CSB-MP726934BAAD
CSB-EP726934BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse COMM domain-containing protein 3 (Commd3) CSB-YP726935MO
CSB-EP726935MO
CSB-BP726935MO
CSB-MP726935MO
CSB-EP726935MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Myosin-11 (Myh11), partial CSB-YP726936RA
CSB-EP726936RA
CSB-BP726936RA
CSB-MP726936RA
CSB-EP726936RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 1-deoxy-D-xylulose 5-phosphate reductoisomerase 1 (dxr1) CSB-YP726937BAAD
CSB-EP726937BAAD
CSB-BP726937BAAD
CSB-MP726937BAAD
CSB-EP726937BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0457 protein BCE33L2961 (BCE33L2961) CSB-YP726938BAAD
CSB-EP726938BAAD
CSB-BP726938BAAD
CSB-MP726938BAAD
CSB-EP726938BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Ras-related protein Rab-3B (Rab3b) CSB-YP726939RA
CSB-EP726939RA
CSB-BP726939RA
CSB-MP726939RA
CSB-EP726939RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cricetulus griseus Dual specificity mitogen-activated protein kinase kinase 1 (MAP2K1) CSB-YP726940DXU
CSB-EP726940DXU
CSB-BP726940DXU
CSB-MP726940DXU
CSB-EP726940DXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Glutamate 5-kinase (proB) CSB-YP726941BAAD
CSB-EP726941BAAD
CSB-BP726941BAAD
CSB-MP726941BAAD
CSB-EP726941BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Kynurenine formamidase (kynB) CSB-YP726942BAAD
CSB-EP726942BAAD
CSB-BP726942BAAD
CSB-MP726942BAAD
CSB-EP726942BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP726943BAAD
CSB-EP726943BAAD
CSB-BP726943BAAD
CSB-MP726943BAAD
CSB-EP726943BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0403 protein BCE33L1972 (BCE33L1972) CSB-YP726944BAAD
CSB-EP726944BAAD
CSB-BP726944BAAD
CSB-MP726944BAAD
CSB-EP726944BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Dihydroxy-acid dehydratase (ilvD), partial CSB-YP726945BAAD
CSB-EP726945BAAD
CSB-BP726945BAAD
CSB-MP726945BAAD
CSB-EP726945BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0398 protein BCE33L1439 (BCE33L1439) CSB-YP726946BAAD
CSB-EP726946BAAD
CSB-BP726946BAAD
CSB-MP726946BAAD
CSB-EP726946BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Dihydrodipicolinate reductase (dapB) CSB-YP726947BAAD
CSB-EP726947BAAD
CSB-BP726947BAAD
CSB-MP726947BAAD
CSB-EP726947BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Histidinol-phosphate aminotransferase 1 (hisC1) CSB-YP726948BAAD
CSB-EP726948BAAD
CSB-BP726948BAAD
CSB-MP726948BAAD
CSB-EP726948BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Demethylmenaquinone methyltransferase (ubiE) CSB-YP726949BAAD
CSB-EP726949BAAD
CSB-BP726949BAAD
CSB-MP726949BAAD
CSB-EP726949BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP726950BAAD
CSB-EP726950BAAD
CSB-BP726950BAAD
CSB-MP726950BAAD
CSB-EP726950BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP726951BAAD
CSB-EP726951BAAD
CSB-BP726951BAAD
CSB-MP726951BAAD
CSB-EP726951BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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