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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mycoplasma hyopneumoniae Chaperone protein DnaJ (dnaJ) CSB-YP726611MWK
CSB-EP726611MWK
CSB-BP726611MWK
CSB-MP726611MWK
CSB-EP726611MWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Copper-containing nitrite reductase (nirK) CSB-YP726612RBAW
CSB-BP726612RBAW
CSB-MP726612RBAW
CSB-EP726612RBAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii UPF0310 protein MJECL36 (MJECL36) CSB-YP726613MRU
CSB-EP726613MRU
CSB-BP726613MRU
CSB-MP726613MRU
CSB-EP726613MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJECL06 (MJECL06) CSB-YP726614MRU
CSB-EP726614MRU
CSB-BP726614MRU
CSB-MP726614MRU
CSB-EP726614MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJECL16 (MJECL16), partial CSB-YP726615MRU1
CSB-EP726615MRU1
CSB-BP726615MRU1
CSB-MP726615MRU1
CSB-EP726615MRU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJECL02 (MJECL02) CSB-YP726616MRU
CSB-EP726616MRU
CSB-BP726616MRU
CSB-MP726616MRU
CSB-EP726616MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJECL11 (MJECL11) CSB-YP726617MRU
CSB-EP726617MRU
CSB-BP726617MRU
CSB-MP726617MRU
CSB-EP726617MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJECL18 (MJECL18) CSB-YP726618MRU
CSB-EP726618MRU
CSB-BP726618MRU
CSB-MP726618MRU
CSB-EP726618MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJECL24 (MJECL24) CSB-YP726619MRU
CSB-EP726619MRU
CSB-BP726619MRU
CSB-MP726619MRU
CSB-EP726619MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJECL32 (MJECL32) CSB-YP726620MRU
CSB-EP726620MRU
CSB-BP726620MRU
CSB-MP726620MRU
CSB-EP726620MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Putative type-1 restriction enzyme MjaXP specificity protein (MJECL41) CSB-YP726621MRU
CSB-EP726621MRU
CSB-BP726621MRU
CSB-MP726621MRU
CSB-EP726621MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma hyopneumoniae tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP726622MWK
CSB-EP726622MWK
CSB-BP726622MWK
CSB-MP726622MWK
CSB-EP726622MWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylococcus capsulatus 3-octaprenyl-4-hydroxybenzoate carboxy-lyase (ubiD) CSB-YP726623MFM
CSB-EP726623MFM
CSB-BP726623MFM
CSB-MP726623MFM
CSB-EP726623MFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylococcus capsulatus 50S ribosomal protein L34 (rpmH) CSB-YP726624MFM
CSB-EP726624MFM
CSB-BP726624MFM
CSB-MP726624MFM
CSB-EP726624MFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylococcus capsulatus Peptide methionine sulfoxide reductase MsrA (msrA) CSB-YP726625MFM
CSB-EP726625MFM
CSB-BP726625MFM
CSB-MP726625MFM
CSB-EP726625MFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Uncharacterized adenine-specific methylase MJECS02 (MJECS02), partial CSB-YP726626MRU
CSB-EP726626MRU
CSB-BP726626MRU
CSB-MP726626MRU
CSB-EP726626MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ0002 (MJ0002) CSB-YP726627MRU
CSB-EP726627MRU
CSB-BP726627MRU
CSB-MP726627MRU
CSB-EP726627MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ0017 (MJ0017) CSB-YP726628MRU
CSB-EP726628MRU
CSB-BP726628MRU
CSB-MP726628MRU
CSB-EP726628MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ0011 (MJ0011) CSB-YP726629MRU
CSB-EP726629MRU
CSB-BP726629MRU
CSB-MP726629MRU
CSB-EP726629MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ0015 (MJ0015) CSB-YP726630MRU
CSB-EP726630MRU
CSB-BP726630MRU
CSB-MP726630MRU
CSB-EP726630MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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