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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Rat F-box only protein 43 (Fbxo43) CSB-YP727722RA
CSB-EP727722RA
CSB-BP727722RA
CSB-MP727722RA
CSB-EP727722RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Serum response factor-binding protein 1 (Srfbp1) CSB-YP727723RA
CSB-EP727723RA
CSB-BP727723RA
CSB-MP727723RA
CSB-EP727723RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Uncharacterized protein C3orf38 homolog CSB-YP727724RA
CSB-EP727724RA
CSB-BP727724RA
CSB-MP727724RA
CSB-EP727724RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Serum deprivation-response protein (Sdpr) CSB-YP727725RA
CSB-EP727725RA
CSB-BP727725RA
CSB-MP727725RA
CSB-EP727725RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Cyclic AMP-responsive element-binding protein 3-like protein 1 (Creb3l1), partial CSB-YP727726RA
CSB-EP727726RA
CSB-BP727726RA
CSB-MP727726RA
CSB-EP727726RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Pyridoxal phosphate phosphatase PHOSPHO2 (Phospho2) CSB-YP727727RA
CSB-EP727727RA
CSB-BP727727RA
CSB-MP727727RA
CSB-EP727727RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Nuclear autoantigenic sperm protein (Nasp) CSB-YP727728RA
CSB-EP727728RA
CSB-BP727728RA
CSB-MP727728RA
CSB-EP727728RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat E3 ubiquitin-protein ligase RNF13 (Rnf13), partial CSB-YP727729RA
CSB-EP727729RA
CSB-BP727729RA
CSB-MP727729RA
CSB-EP727729RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Solute carrier family 35 member B1 (slc35b1), partial CSB-YP727730DIL
CSB-EP727730DIL
CSB-BP727730DIL
CSB-MP727730DIL
CSB-EP727730DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Transmembrane protein 55B-B (tmem55bb), partial CSB-YP727731DIL1
CSB-EP727731DIL1
CSB-BP727731DIL1
CSB-MP727731DIL1
CSB-EP727731DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Coiled-coil domain-containing protein 130 homolog (ccdc130) CSB-YP727732DIL
CSB-EP727732DIL
CSB-BP727732DIL
CSB-MP727732DIL
CSB-EP727732DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis U1 small nuclear ribonucleoprotein 70 kDa (snrnp70) CSB-YP727733XBF
CSB-EP727733XBF
CSB-BP727733XBF
CSB-MP727733XBF
CSB-EP727733XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Glutamine and serine-rich protein 1 (qser1), partial CSB-YP727734XBE
CSB-EP727734XBE
CSB-BP727734XBE
CSB-MP727734XBE
CSB-EP727734XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein wntless homolog A (wls-a), partial CSB-YP727735XBE1
CSB-EP727735XBE1
CSB-BP727735XBE1
CSB-MP727735XBE1
CSB-EP727735XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Dynactin subunit 2-A (dctn2-a) CSB-YP727736XBE
CSB-EP727736XBE
CSB-BP727736XBE
CSB-MP727736XBE
CSB-EP727736XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Tuftelin-interacting protein 11 (tfip11), partial CSB-YP727737XBE
CSB-EP727737XBE
CSB-BP727737XBE
CSB-MP727737XBE
CSB-EP727737XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis E3 ubiquitin-protein ligase arkadia-C (rnf111-c), partial CSB-YP727738XBE
CSB-EP727738XBE
CSB-BP727738XBE
CSB-MP727738XBE
CSB-EP727738XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Enhancer of polycomb homolog 2 (epc2), partial CSB-YP727739XBE
CSB-EP727739XBE
CSB-BP727739XBE
CSB-MP727739XBE
CSB-EP727739XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Probable alpha-ketoglutarate-dependent dioxygenase ABH5 (alkbh5) CSB-YP727740XBF
CSB-EP727740XBF
CSB-BP727740XBF
CSB-MP727740XBF
CSB-EP727740XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Nuclear receptor subfamily 6 group A member 1 (nr6a1) CSB-YP727741XBF
CSB-EP727741XBF
CSB-BP727741XBF
CSB-MP727741XBF
CSB-EP727741XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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