重組蛋白
重組蛋白質(zhì)是分析生物相互作用的有用工具。在研究實(shí)驗(yàn)室中使用了大量的重組蛋白質(zhì),包括商業(yè)蛋白質(zhì)和在特定研究項(xiàng)目中生成的蛋白質(zhì)。它們?cè)谠S多應(yīng)用中發(fā)揮著關(guān)鍵作用。
如果您正在尋找具有優(yōu)良質(zhì)量(驗(yàn)證的生物活性,較低的內(nèi)毒素水平和高純度)的重組蛋白質(zhì)來支持您的研究,您來對(duì)地方了。
經(jīng)過16多年的發(fā)展,華美生物已經(jīng)建立了從原核到真核的五大系統(tǒng),用于表達(dá)重組蛋白質(zhì)。我們擁有一系列蛋白質(zhì)表達(dá)和純化技術(shù),包括基于桿狀病毒外膜蛋白(OMP)的蛋白質(zhì)表達(dá)技術(shù),以及膜蛋白表達(dá)技術(shù)等。
產(chǎn)品特點(diǎn)
- 超過10,000種重組蛋白覆蓋大多數(shù)基因/靶點(diǎn);
- 5種表達(dá)系統(tǒng),包括大腸桿菌(E.coli)、酵母、桿狀病毒感染的昆蟲細(xì)胞、哺乳動(dòng)物細(xì)胞和體外大腸桿菌。而體外大腸桿菌表達(dá)系統(tǒng)是華美生物的獨(dú)特表達(dá)系統(tǒng)。與傳統(tǒng)蛋白表達(dá)系統(tǒng)相比,體外大腸桿菌省略了許多過程,如質(zhì)粒轉(zhuǎn)化、細(xì)胞培養(yǎng)、收集、破碎和離心等,大大提高了工作效率。
- 多種標(biāo)簽,包括His、GST、Flag、MBP等。
- 廣泛應(yīng)用,包括抗原、細(xì)胞分析、結(jié)合分析/蛋白質(zhì)相互作用、細(xì)胞培養(yǎng)-無血清培養(yǎng)基、藥物相關(guān)研究、體外酶活性、ELISA標(biāo)準(zhǔn)及其原料、體內(nèi)研究、質(zhì)譜標(biāo)準(zhǔn)、蛋白芯片、SDS-PAGE控制、蛋白質(zhì)結(jié)構(gòu)分析(晶體/電子顯微鏡)。
- 多種物種,包括人類、小鼠、大鼠、兔子、豚鼠、雞、猴子、豬等。
- 無動(dòng)物副產(chǎn)品的動(dòng)物源性重組蛋白質(zhì)。華美生物已開發(fā)出無動(dòng)物副產(chǎn)品接觸的重組蛋白質(zhì)產(chǎn)品線。
重組蛋白目錄
以下是所有重組蛋白的目錄。您可以在目錄中找到適合您研究的蛋白質(zhì),或在本網(wǎng)站頭部的搜索框中搜索它們。
如果您找不到您需要的抗體,請(qǐng)查看華美生物蛋白質(zhì)定制服務(wù)。
通過選擇目標(biāo)名稱的首字母瀏覽產(chǎn)品。
產(chǎn)品名稱 | 貨號(hào) | 規(guī)格 |
---|---|---|
Recombinant Pongo abelii ATP synthase subunit f, mitochondrial (ATP5J2), partial | CSB-YP728936PYX1 CSB-EP728936PYX1 CSB-BP728936PYX1 CSB-MP728936PYX1 CSB-EP728936PYX1-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii Dihydroorotate dehydrogenase (quinone), mitochondrial (DHODH), partial | CSB-YP728937PYX1 CSB-EP728937PYX1 CSB-BP728937PYX1 CSB-MP728937PYX1 CSB-EP728937PYX1-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii Spermatid perinuclear RNA-binding protein (STRBP), partial | CSB-YP728938PYX CSB-EP728938PYX CSB-BP728938PYX CSB-MP728938PYX CSB-EP728938PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii Cell differentiation protein RCD1 homolog (RQCD1) | CSB-YP728939PYX CSB-EP728939PYX CSB-BP728939PYX CSB-MP728939PYX CSB-EP728939PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii High mobility group nucleosome-binding domain-containing protein 3 (HMGN3) | CSB-YP728940PYX CSB-EP728940PYX CSB-BP728940PYX CSB-MP728940PYX CSB-EP728940PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii Josephin-1 (JOSD1) | CSB-YP728941PYX CSB-EP728941PYX CSB-BP728941PYX CSB-MP728941PYX CSB-EP728941PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii Tripartite motif-containing protein 16 (TRIM16), partial | CSB-YP728942PYX CSB-EP728942PYX CSB-BP728942PYX CSB-MP728942PYX CSB-EP728942PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii Iron-sulfur cluster assembly 2 homolog, mitochondrial (ISCA2) | CSB-YP728943PYX CSB-EP728943PYX CSB-BP728943PYX CSB-MP728943PYX CSB-EP728943PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii Chitobiosyldiphosphodolichol beta-mannosyltransferase (ALG1), partial | CSB-YP728944PYX CSB-EP728944PYX CSB-BP728944PYX CSB-MP728944PYX CSB-EP728944PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii UDP-glucose 6-dehydrogenase (UGDH) | CSB-YP728945PYX CSB-EP728945PYX CSB-BP728945PYX CSB-MP728945PYX CSB-EP728945PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii Kelch-like protein 20 (KLHL20), partial | CSB-YP728946PYX CSB-EP728946PYX CSB-BP728946PYX CSB-MP728946PYX CSB-EP728946PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii ATP-dependent RNA helicase DDX42 (DDX42), partial | CSB-YP728947PYX CSB-EP728947PYX CSB-BP728947PYX CSB-MP728947PYX CSB-EP728947PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii Peroxiredoxin-6 (PRDX6) | CSB-YP728948PYX CSB-EP728948PYX CSB-BP728948PYX CSB-MP728948PYX CSB-EP728948PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii Zinc fingers and homeoboxes protein 2 (ZHX2), partial | CSB-YP728949PYX CSB-EP728949PYX CSB-BP728949PYX CSB-MP728949PYX CSB-EP728949PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii MAP7 domain-containing protein 2 (MAP7D2), partial | CSB-YP728950PYX CSB-EP728950PYX CSB-BP728950PYX CSB-MP728950PYX CSB-EP728950PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii Probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, mitochondrial (DHTKD1), partial | CSB-YP728951PYX CSB-EP728951PYX CSB-BP728951PYX CSB-MP728951PYX CSB-EP728951PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii Nuclear envelope phosphatase-regulatory subunit 1 (CNEP1R1), partial | CSB-YP728952PYX1 CSB-EP728952PYX1 CSB-BP728952PYX1 CSB-MP728952PYX1 CSB-EP728952PYX1-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii Ras-related protein Rab-5C (RAB5C) | CSB-YP728953PYX CSB-EP728953PYX CSB-BP728953PYX CSB-MP728953PYX CSB-EP728953PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii Bax inhibitor 1 (TMBIM6), partial | CSB-YP728954PYX1 CSB-EP728954PYX1 CSB-BP728954PYX1 CSB-MP728954PYX1 CSB-EP728954PYX1-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii AN1-type zinc finger protein 6 (ZFAND6) | CSB-YP728955PYX CSB-EP728955PYX CSB-BP728955PYX CSB-MP728955PYX CSB-EP728955PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
有用資源
什么是重組蛋白?
重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。
第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。
第二條:如何選擇合適的表達(dá)載體?
表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。
根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。
引用資料
Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023
Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023
Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023
Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023
Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022