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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Leifsonia xyli subsp. xyli GTPase Der (der) CSB-YP728042LAAC
CSB-EP728042LAAC
CSB-BP728042LAAC
CSB-MP728042LAAC
CSB-EP728042LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli Glycerol kinase (glpK), partial CSB-YP728043LAAC
CSB-EP728043LAAC
CSB-BP728043LAAC
CSB-MP728043LAAC
CSB-EP728043LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli 50S ribosomal protein L10 (rplJ) CSB-YP728044LAAC
CSB-EP728044LAAC
CSB-BP728044LAAC
CSB-MP728044LAAC
CSB-EP728044LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP728045LAAC
CSB-EP728045LAAC
CSB-BP728045LAAC
CSB-MP728045LAAC
CSB-EP728045LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli Protein CrcB homolog 1 (crcB1), partial CSB-YP728046LAAC1
CSB-EP728046LAAC1
CSB-BP728046LAAC1
CSB-MP728046LAAC1
CSB-EP728046LAAC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila tRNA (mo5U34)-methyltransferase (cmoB) CSB-YP728047DAAA
CSB-EP728047DAAA
CSB-BP728047DAAA
CSB-MP728047DAAA
CSB-EP728047DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP728048DAAA
CSB-EP728048DAAA
CSB-BP728048DAAA
CSB-MP728048DAAA
CSB-EP728048DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP728049DAAA
CSB-EP728049DAAA
CSB-BP728049DAAA
CSB-MP728049DAAA
CSB-EP728049DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Ribonuclease Y (rny), partial CSB-YP728050DAAA
CSB-EP728050DAAA
CSB-BP728050DAAA
CSB-MP728050DAAA
CSB-EP728050DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila 50S ribosomal protein L19 (rplS) CSB-YP728051DAAA
CSB-EP728051DAAA
CSB-BP728051DAAA
CSB-MP728051DAAA
CSB-EP728051DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Phosphatidylserine decarboxylase proenzyme (psd) CSB-YP728052DAAA
CSB-EP728052DAAA
CSB-BP728052DAAA
CSB-MP728052DAAA
CSB-EP728052DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Peptidyl-tRNA hydrolase (pth) CSB-YP728053DAAA
CSB-EP728053DAAA
CSB-BP728053DAAA
CSB-MP728053DAAA
CSB-EP728053DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP728054DAAA
CSB-EP728054DAAA
CSB-BP728054DAAA
CSB-MP728054DAAA
CSB-EP728054DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Lipid A export ATP-binding/permease protein MsbA (msbA), partial CSB-YP728055DAAA1
CSB-EP728055DAAA1
CSB-BP728055DAAA1
CSB-MP728055DAAA1
CSB-EP728055DAAA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Leucine--tRNA ligase (leuS), partial CSB-YP728056DAAA
CSB-EP728056DAAA
CSB-BP728056DAAA
CSB-MP728056DAAA
CSB-EP728056DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Gamma-glutamyl phosphate reductase (proA) CSB-YP728057DAAA
CSB-EP728057DAAA
CSB-BP728057DAAA
CSB-MP728057DAAA
CSB-EP728057DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Lon protease 2 (lon2), partial CSB-YP728058DAAA
CSB-EP728058DAAA
CSB-BP728058DAAA
CSB-MP728058DAAA
CSB-EP728058DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Ribosomal protein S6 modification protein (rimK) CSB-YP728059DAAA
CSB-EP728059DAAA
CSB-BP728059DAAA
CSB-MP728059DAAA
CSB-EP728059DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila UPF0301 protein DP2218 (DP2218) CSB-YP728060DAAA
CSB-EP728060DAAA
CSB-BP728060DAAA
CSB-MP728060DAAA
CSB-EP728060DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP728061DAAA
CSB-EP728061DAAA
CSB-BP728061DAAA
CSB-MP728061DAAA
CSB-EP728061DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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