Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Yarrowia lipolytica Protein CSN12 homolog (CSN12) CSB-YP728243YAP
CSB-EP728243YAP
CSB-BP728243YAP
CSB-MP728243YAP
CSB-EP728243YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica GPI mannosyltransferase 2 (GPI18), partial CSB-YP728244YAP
CSB-EP728244YAP
CSB-BP728244YAP
CSB-MP728244YAP
CSB-EP728244YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Serine/threonine-protein kinase BUD32 (BUD32) CSB-YP728245YAP
CSB-EP728245YAP
CSB-BP728245YAP
CSB-MP728245YAP
CSB-EP728245YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Mitochondrial Rho GTPase 1 (GEM1), partial CSB-YP728246YAP
CSB-EP728246YAP
CSB-BP728246YAP
CSB-MP728246YAP
CSB-EP728246YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Protein transport protein SEC24 (SEC24), partial CSB-YP728247YAP
CSB-EP728247YAP
CSB-BP728247YAP
CSB-MP728247YAP
CSB-EP728247YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Mitochondrial import inner membrane translocase subunit TIM16 (PAM16) CSB-YP728248YAP
CSB-EP728248YAP
CSB-BP728248YAP
CSB-MP728248YAP
CSB-EP728248YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Glutamine synthetase (GLN1) CSB-YP728249YAP
CSB-EP728249YAP
CSB-BP728249YAP
CSB-MP728249YAP
CSB-EP728249YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica ATPase GET3 (GET3) CSB-YP728250YAP
CSB-EP728250YAP
CSB-BP728250YAP
CSB-MP728250YAP
CSB-EP728250YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica RuvB-like helicase 2 (RVB2) CSB-YP728251YAP
CSB-EP728251YAP
CSB-BP728251YAP
CSB-MP728251YAP
CSB-EP728251YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Increased rDNA silencing protein 4 (IRS4), partial CSB-YP728252YAP
CSB-EP728252YAP
CSB-BP728252YAP
CSB-MP728252YAP
CSB-EP728252YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica tRNA guanosine-2'-O-methyltransferase TRM13 (TRM13) CSB-YP728253YAP
CSB-EP728253YAP
CSB-BP728253YAP
CSB-MP728253YAP
CSB-EP728253YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Pheromone-regulated membrane protein 10 (YALI0E24651g), partial CSB-YP728254YAP
CSB-EP728254YAP
CSB-BP728254YAP
CSB-MP728254YAP
CSB-EP728254YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Palmitoyltransferase PFA3 (PFA3), partial CSB-YP728255YAP1
CSB-EP728255YAP1
CSB-BP728255YAP1
CSB-MP728255YAP1
CSB-EP728255YAP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Palmitoyltransferase AKR1 (AKR1), partial CSB-YP728256YAP1
CSB-EP728256YAP1
CSB-BP728256YAP1
CSB-MP728256YAP1
CSB-EP728256YAP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Transcription activator of gluconeogenesis ERT1-1 (ERT1-1) CSB-YP728257YAP
CSB-EP728257YAP
CSB-BP728257YAP
CSB-MP728257YAP
CSB-EP728257YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Polynucleotide 5'-hydroxyl-kinase GRC3 (GRC3), partial CSB-YP728258YAP
CSB-EP728258YAP
CSB-BP728258YAP
CSB-MP728258YAP
CSB-EP728258YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Arginine biosynthesis bifunctional protein ArgJ, mitochondrial (YALI0E13057g), partial CSB-YP728259YAP
CSB-EP728259YAP
CSB-BP728259YAP
CSB-MP728259YAP
CSB-EP728259YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Protein DML1 (DML1) CSB-YP728260YAP
CSB-EP728260YAP
CSB-BP728260YAP
CSB-MP728260YAP
CSB-EP728260YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Diphthamide biosynthesis protein 4 (DPH4) CSB-YP728261YAP
CSB-EP728261YAP
CSB-BP728261YAP
CSB-MP728261YAP
CSB-EP728261YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Pre-mRNA-splicing factor PRP46 (PRP46) CSB-YP728262YAP
CSB-EP728262YAP
CSB-BP728262YAP
CSB-MP728262YAP
CSB-EP728262YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>