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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Saccharum officinarum Uncharacterized protein ycf72 (ycf72-1) CSB-YP728603SVV
CSB-EP728603SVV
CSB-BP728603SVV
CSB-MP728603SVV
CSB-EP728603SVV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharum officinarum 50S ribosomal protein L36, chloroplastic (rpl36) CSB-YP728604SVV
CSB-EP728604SVV
CSB-BP728604SVV
CSB-MP728604SVV
CSB-EP728604SVV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharum officinarum Photosystem II CP47 chlorophyll apoprotein (psbB), partial CSB-YP728605SVV1
CSB-EP728605SVV1
CSB-BP728605SVV1
CSB-MP728605SVV1
CSB-EP728605SVV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharum officinarum Cytochrome b559 subunit alpha (psbE), partial CSB-YP728606SVV1
CSB-EP728606SVV1
CSB-BP728606SVV1
CSB-MP728606SVV1
CSB-EP728606SVV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharum officinarum Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP728607SVV
CSB-EP728607SVV
CSB-BP728607SVV
CSB-MP728607SVV
CSB-EP728607SVV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharum officinarum ATP synthase subunit b, chloroplastic (atpF), partial CSB-YP728608SVV1
CSB-EP728608SVV1
CSB-BP728608SVV1
CSB-MP728608SVV1
CSB-EP728608SVV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharum officinarum Photosystem II reaction center protein M (psbM), partial CSB-YP728609SVV
CSB-EP728609SVV
CSB-BP728609SVV
CSB-MP728609SVV
CSB-EP728609SVV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharum officinarum Photosystem II reaction center protein I (psbI), partial CSB-YP728610SVV
CSB-EP728610SVV
CSB-BP728610SVV
CSB-MP728610SVV
CSB-EP728610SVV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica SPX domain-containing membrane protein Os02g45520 (Os02g0678200, LOC_Os02g45520), partial CSB-YP728611OFG
CSB-EP728611OFG
CSB-BP728611OFG
CSB-MP728611OFG
CSB-EP728611OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Glutelin type-B 5 (GLUB5) CSB-YP728612OFG
CSB-EP728612OFG
CSB-BP728612OFG
CSB-MP728612OFG
CSB-EP728612OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Germin-like protein 2-4 (Os02g0532500, LOC_Os02g32980) CSB-YP728613OFG
CSB-EP728613OFG
CSB-BP728613OFG
CSB-MP728613OFG
CSB-EP728613OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable 4-coumarate--CoA ligase 3 (4CL3), partial CSB-YP728614OFG
CSB-EP728614OFG
CSB-BP728614OFG
CSB-MP728614OFG
CSB-EP728614OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica U-box domain-containing protein 4 (PUB4), partial CSB-YP728615OFG
CSB-EP728615OFG
CSB-BP728615OFG
CSB-MP728615OFG
CSB-EP728615OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes GDP-fucose protein O-fucosyltransferase 1 (POFUT1) CSB-YP728616EQV
CSB-EP728616EQV
CSB-BP728616EQV
CSB-MP728616EQV
CSB-EP728616EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Suillus bovinus Tubulin beta-1 chain (TUBB1) CSB-YP728617SFAP
CSB-EP728617SFAP
CSB-BP728617SFAP
CSB-MP728617SFAP
CSB-EP728617SFAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nymphaea alba 30S ribosomal protein S15, chloroplastic (rps15) CSB-YP728618NES
CSB-EP728618NES
CSB-BP728618NES
CSB-MP728618NES
CSB-EP728618NES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nymphaea alba Cytochrome c biogenesis protein ccsA (ccsA), partial CSB-YP728619NES1
CSB-EP728619NES1
CSB-BP728619NES1
CSB-MP728619NES1
CSB-EP728619NES1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nymphaea alba 50S ribosomal protein L16, chloroplastic (rpl16) CSB-YP728620NES
CSB-EP728620NES
CSB-BP728620NES
CSB-MP728620NES
CSB-EP728620NES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nymphaea alba Photosystem II reaction center protein H (psbH), partial CSB-YP728621NES1
CSB-EP728621NES1
CSB-BP728621NES1
CSB-MP728621NES1
CSB-EP728621NES1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nymphaea alba 50S ribosomal protein L33, chloroplastic (rpl33) CSB-YP728622NES
CSB-EP728622NES
CSB-BP728622NES
CSB-MP728622NES
CSB-EP728622NES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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