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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus cereus Protein hfq (hfq) CSB-YP730922BAAD
CSB-EP730922BAAD
CSB-BP730922BAAD
CSB-MP730922BAAD
CSB-EP730922BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Protein jagged-1 (Jag1), partial CSB-YP730923RA
CSB-EP730923RA
CSB-BP730923RA
CSB-MP730923RA
CSB-EP730923RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Potassium voltage-gated channel subfamily C member 4 (Kcnc4), partial CSB-YP730924RA1
CSB-EP730924RA1
CSB-BP730924RA1
CSB-MP730924RA1
CSB-EP730924RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Phosphatidylinositol 3-kinase regulatory subunit beta (Pik3r2) CSB-YP730925RA
CSB-EP730925RA
CSB-BP730925RA
CSB-MP730925RA
CSB-EP730925RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Holin-like protein CidA (cidA), partial CSB-YP730926BAAD1
CSB-EP730926BAAD1
CSB-BP730926BAAD1
CSB-MP730926BAAD1
CSB-EP730926BAAD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Glycerol-3-phosphate acyltransferase 2 (plsY2), partial CSB-YP730927BAAD1
CSB-EP730927BAAD1
CSB-BP730927BAAD1
CSB-MP730927BAAD1
CSB-EP730927BAAD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Calcineurin subunit B type 2 (Ppp3r2) CSB-YP730928MO
CSB-EP730928MO
CSB-BP730928MO
CSB-MP730928MO
CSB-EP730928MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Histidine--tRNA ligase 1 (hisS1) CSB-YP730929BAAD
CSB-EP730929BAAD
CSB-BP730929BAAD
CSB-MP730929BAAD
CSB-EP730929BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Protein SET (Set) CSB-YP730930RA
CSB-EP730930RA
CSB-BP730930RA
CSB-MP730930RA
CSB-EP730930RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Myeloid cell surface antigen CD33 (Cd33), partial CSB-YP730931MO
CSB-EP730931MO
CSB-BP730931MO
CSB-MP730931MO
CSB-EP730931MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP730932BAAD
CSB-EP730932BAAD
CSB-BP730932BAAD
CSB-MP730932BAAD
CSB-EP730932BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 5-dehydro-2-deoxygluconokinase 1 (iolC1) CSB-YP730933BAAD
CSB-EP730933BAAD
CSB-BP730933BAAD
CSB-MP730933BAAD
CSB-EP730933BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus FMN-dependent NADH-azoreductase 3 (azoR3) CSB-YP730934BAAD
CSB-EP730934BAAD
CSB-BP730934BAAD
CSB-MP730934BAAD
CSB-EP730934BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus L-lactate dehydrogenase 1 (ldh1) CSB-YP730935BAAD
CSB-EP730935BAAD
CSB-BP730935BAAD
CSB-MP730935BAAD
CSB-EP730935BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 5-methylthioadenosine/S-adenosylhomocysteine deaminase (mtaD) CSB-YP730936BAAD
CSB-EP730936BAAD
CSB-BP730936BAAD
CSB-MP730936BAAD
CSB-EP730936BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Ketol-acid reductoisomerase 2 (ilvC2) CSB-YP730937BAAD
CSB-EP730937BAAD
CSB-BP730937BAAD
CSB-MP730937BAAD
CSB-EP730937BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Holliday junction resolvase recU (recU) CSB-YP730938BAAD
CSB-EP730938BAAD
CSB-BP730938BAAD
CSB-MP730938BAAD
CSB-EP730938BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Cytidylate kinase (cmk) CSB-YP730939BAAD
CSB-EP730939BAAD
CSB-BP730939BAAD
CSB-MP730939BAAD
CSB-EP730939BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphoadenosine phosphosulfate reductase (cysH) CSB-YP730940BAAD
CSB-EP730940BAAD
CSB-BP730940BAAD
CSB-MP730940BAAD
CSB-EP730940BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP730941BAAD
CSB-EP730941BAAD
CSB-BP730941BAAD
CSB-MP730941BAAD
CSB-EP730941BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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