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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mannheimia succiniciproducens UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) CSB-YP731243MAAB
CSB-EP731243MAAB
CSB-BP731243MAAB
CSB-MP731243MAAB
CSB-EP731243MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Gamma-glutamyl phosphate reductase (proA) CSB-YP731244MAAB
CSB-EP731244MAAB
CSB-BP731244MAAB
CSB-MP731244MAAB
CSB-EP731244MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens GTPase obg (obg) CSB-YP731245MAAB
CSB-EP731245MAAB
CSB-BP731245MAAB
CSB-MP731245MAAB
CSB-EP731245MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens UPF0265 protein MS1572 (MS1572) CSB-YP731246MAAB
CSB-EP731246MAAB
CSB-BP731246MAAB
CSB-MP731246MAAB
CSB-EP731246MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Queuine tRNA-ribosyltransferase (tgt) CSB-YP731247MAAB
CSB-EP731247MAAB
CSB-BP731247MAAB
CSB-MP731247MAAB
CSB-EP731247MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP731248MAAB
CSB-EP731248MAAB
CSB-BP731248MAAB
CSB-MP731248MAAB
CSB-EP731248MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Thiamine import ATP-binding protein ThiQ (thiQ) CSB-YP731249MAAB
CSB-EP731249MAAB
CSB-BP731249MAAB
CSB-MP731249MAAB
CSB-EP731249MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens tRNA dimethylallyltransferase (miaA) CSB-YP731250MAAB
CSB-EP731250MAAB
CSB-BP731250MAAB
CSB-MP731250MAAB
CSB-EP731250MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens UPF0133 protein MS1507 (MS1507) CSB-YP731251MAAB
CSB-EP731251MAAB
CSB-BP731251MAAB
CSB-MP731251MAAB
CSB-EP731251MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Argininosuccinate synthase (argG) CSB-YP731252MAAB
CSB-EP731252MAAB
CSB-BP731252MAAB
CSB-MP731252MAAB
CSB-EP731252MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Ribosome maturation factor RimP (rimP) CSB-YP731253MAAB
CSB-EP731253MAAB
CSB-BP731253MAAB
CSB-MP731253MAAB
CSB-EP731253MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Glutamyl-tRNA reductase (hemA) CSB-YP731254MAAB
CSB-EP731254MAAB
CSB-BP731254MAAB
CSB-MP731254MAAB
CSB-EP731254MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Probable multidrug resistance protein norM (norM), partial CSB-YP731255MAAB
CSB-EP731255MAAB
CSB-BP731255MAAB
CSB-MP731255MAAB
CSB-EP731255MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Penicillin-binding protein activator LpoA (lpoA), partial CSB-YP731256MAAB
CSB-EP731256MAAB
CSB-BP731256MAAB
CSB-MP731256MAAB
CSB-EP731256MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Sulfite reductase [NADPH] hemoprotein beta-component (cysI), partial CSB-YP731257MAAB
CSB-EP731257MAAB
CSB-BP731257MAAB
CSB-MP731257MAAB
CSB-EP731257MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Lipoprotein-releasing system ATP-binding protein LolD (lolD) CSB-YP731258MAAB
CSB-EP731258MAAB
CSB-BP731258MAAB
CSB-MP731258MAAB
CSB-EP731258MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Anthranilate phosphoribosyltransferase (trpD) CSB-YP731259MAAB
CSB-EP731259MAAB
CSB-BP731259MAAB
CSB-MP731259MAAB
CSB-EP731259MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP731260MAAB
CSB-EP731260MAAB
CSB-BP731260MAAB
CSB-MP731260MAAB
CSB-EP731260MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP731261MAAB
CSB-EP731261MAAB
CSB-BP731261MAAB
CSB-MP731261MAAB
CSB-EP731261MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP731262MAAB
CSB-EP731262MAAB
CSB-BP731262MAAB
CSB-MP731262MAAB
CSB-EP731262MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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