重組蛋白
重組蛋白質(zhì)是分析生物相互作用的有用工具。在研究實(shí)驗(yàn)室中使用了大量的重組蛋白質(zhì),包括商業(yè)蛋白質(zhì)和在特定研究項(xiàng)目中生成的蛋白質(zhì)。它們?cè)谠S多應(yīng)用中發(fā)揮著關(guān)鍵作用。
如果您正在尋找具有優(yōu)良質(zhì)量(驗(yàn)證的生物活性,較低的內(nèi)毒素水平和高純度)的重組蛋白質(zhì)來(lái)支持您的研究,您來(lái)對(duì)地方了。
經(jīng)過(guò)16多年的發(fā)展,華美生物已經(jīng)建立了從原核到真核的五大系統(tǒng),用于表達(dá)重組蛋白質(zhì)。我們擁有一系列蛋白質(zhì)表達(dá)和純化技術(shù),包括基于桿狀病毒外膜蛋白(OMP)的蛋白質(zhì)表達(dá)技術(shù),以及膜蛋白表達(dá)技術(shù)等。
產(chǎn)品特點(diǎn)
- 超過(guò)10,000種重組蛋白覆蓋大多數(shù)基因/靶點(diǎn);
- 5種表達(dá)系統(tǒng),包括大腸桿菌(E.coli)、酵母、桿狀病毒感染的昆蟲(chóng)細(xì)胞、哺乳動(dòng)物細(xì)胞和體外大腸桿菌。而體外大腸桿菌表達(dá)系統(tǒng)是華美生物的獨(dú)特表達(dá)系統(tǒng)。與傳統(tǒng)蛋白表達(dá)系統(tǒng)相比,體外大腸桿菌省略了許多過(guò)程,如質(zhì)粒轉(zhuǎn)化、細(xì)胞培養(yǎng)、收集、破碎和離心等,大大提高了工作效率。
- 多種標(biāo)簽,包括His、GST、Flag、MBP等。
- 廣泛應(yīng)用,包括抗原、細(xì)胞分析、結(jié)合分析/蛋白質(zhì)相互作用、細(xì)胞培養(yǎng)-無(wú)血清培養(yǎng)基、藥物相關(guān)研究、體外酶活性、ELISA標(biāo)準(zhǔn)及其原料、體內(nèi)研究、質(zhì)譜標(biāo)準(zhǔn)、蛋白芯片、SDS-PAGE控制、蛋白質(zhì)結(jié)構(gòu)分析(晶體/電子顯微鏡)。
- 多種物種,包括人類、小鼠、大鼠、兔子、豚鼠、雞、猴子、豬等。
- 無(wú)動(dòng)物副產(chǎn)品的動(dòng)物源性重組蛋白質(zhì)。華美生物已開(kāi)發(fā)出無(wú)動(dòng)物副產(chǎn)品接觸的重組蛋白質(zhì)產(chǎn)品線。
重組蛋白目錄
以下是所有重組蛋白的目錄。您可以在目錄中找到適合您研究的蛋白質(zhì),或在本網(wǎng)站頭部的搜索框中搜索它們。
如果您找不到您需要的抗體,請(qǐng)查看華美生物蛋白質(zhì)定制服務(wù)。
通過(guò)選擇目標(biāo)名稱的首字母瀏覽產(chǎn)品。
產(chǎn)品名稱 | 貨號(hào) | 規(guī)格 |
---|---|---|
Recombinant Mannheimia succiniciproducens UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) | CSB-YP731243MAAB CSB-EP731243MAAB CSB-BP731243MAAB CSB-MP731243MAAB CSB-EP731243MAAB-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Mannheimia succiniciproducens Gamma-glutamyl phosphate reductase (proA) | CSB-YP731244MAAB CSB-EP731244MAAB CSB-BP731244MAAB CSB-MP731244MAAB CSB-EP731244MAAB-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Mannheimia succiniciproducens GTPase obg (obg) | CSB-YP731245MAAB CSB-EP731245MAAB CSB-BP731245MAAB CSB-MP731245MAAB CSB-EP731245MAAB-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Mannheimia succiniciproducens UPF0265 protein MS1572 (MS1572) | CSB-YP731246MAAB CSB-EP731246MAAB CSB-BP731246MAAB CSB-MP731246MAAB CSB-EP731246MAAB-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Mannheimia succiniciproducens Queuine tRNA-ribosyltransferase (tgt) | CSB-YP731247MAAB CSB-EP731247MAAB CSB-BP731247MAAB CSB-MP731247MAAB CSB-EP731247MAAB-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Mannheimia succiniciproducens 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) | CSB-YP731248MAAB CSB-EP731248MAAB CSB-BP731248MAAB CSB-MP731248MAAB CSB-EP731248MAAB-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Mannheimia succiniciproducens Thiamine import ATP-binding protein ThiQ (thiQ) | CSB-YP731249MAAB CSB-EP731249MAAB CSB-BP731249MAAB CSB-MP731249MAAB CSB-EP731249MAAB-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Mannheimia succiniciproducens tRNA dimethylallyltransferase (miaA) | CSB-YP731250MAAB CSB-EP731250MAAB CSB-BP731250MAAB CSB-MP731250MAAB CSB-EP731250MAAB-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Mannheimia succiniciproducens UPF0133 protein MS1507 (MS1507) | CSB-YP731251MAAB CSB-EP731251MAAB CSB-BP731251MAAB CSB-MP731251MAAB CSB-EP731251MAAB-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Mannheimia succiniciproducens Argininosuccinate synthase (argG) | CSB-YP731252MAAB CSB-EP731252MAAB CSB-BP731252MAAB CSB-MP731252MAAB CSB-EP731252MAAB-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Mannheimia succiniciproducens Ribosome maturation factor RimP (rimP) | CSB-YP731253MAAB CSB-EP731253MAAB CSB-BP731253MAAB CSB-MP731253MAAB CSB-EP731253MAAB-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Mannheimia succiniciproducens Glutamyl-tRNA reductase (hemA) | CSB-YP731254MAAB CSB-EP731254MAAB CSB-BP731254MAAB CSB-MP731254MAAB CSB-EP731254MAAB-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Mannheimia succiniciproducens Probable multidrug resistance protein norM (norM), partial | CSB-YP731255MAAB CSB-EP731255MAAB CSB-BP731255MAAB CSB-MP731255MAAB CSB-EP731255MAAB-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Mannheimia succiniciproducens Penicillin-binding protein activator LpoA (lpoA), partial | CSB-YP731256MAAB CSB-EP731256MAAB CSB-BP731256MAAB CSB-MP731256MAAB CSB-EP731256MAAB-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Mannheimia succiniciproducens Sulfite reductase [NADPH] hemoprotein beta-component (cysI), partial | CSB-YP731257MAAB CSB-EP731257MAAB CSB-BP731257MAAB CSB-MP731257MAAB CSB-EP731257MAAB-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Mannheimia succiniciproducens Lipoprotein-releasing system ATP-binding protein LolD (lolD) | CSB-YP731258MAAB CSB-EP731258MAAB CSB-BP731258MAAB CSB-MP731258MAAB CSB-EP731258MAAB-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Mannheimia succiniciproducens Anthranilate phosphoribosyltransferase (trpD) | CSB-YP731259MAAB CSB-EP731259MAAB CSB-BP731259MAAB CSB-MP731259MAAB CSB-EP731259MAAB-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Mannheimia succiniciproducens tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) | CSB-YP731260MAAB CSB-EP731260MAAB CSB-BP731260MAAB CSB-MP731260MAAB CSB-EP731260MAAB-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Mannheimia succiniciproducens 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial | CSB-YP731261MAAB CSB-EP731261MAAB CSB-BP731261MAAB CSB-MP731261MAAB CSB-EP731261MAAB-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Mannheimia succiniciproducens Molybdenum cofactor biosynthesis protein C (moaC) | CSB-YP731262MAAB CSB-EP731262MAAB CSB-BP731262MAAB CSB-MP731262MAAB CSB-EP731262MAAB-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
有用資源
什么是重組蛋白?
重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。
第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。
第二條:如何選擇合適的表達(dá)載體?
表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。
根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。
引用資料
Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023
Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023
Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023
Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023
Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022