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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Legionella pneumophila LPS-assembly protein lptD (lptD), partial CSB-YP730301LAAB
CSB-EP730301LAAB
CSB-BP730301LAAB
CSB-MP730301LAAB
CSB-EP730301LAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Protease HtpX (htpX), partial CSB-YP730302LAAB1
CSB-EP730302LAAB1
CSB-BP730302LAAB1
CSB-MP730302LAAB1
CSB-EP730302LAAB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Glycine cleavage system H protein (gcvH) CSB-YP730303LAAB
CSB-EP730303LAAB
CSB-BP730303LAAB
CSB-MP730303LAAB
CSB-EP730303LAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Inosine-5'-monophosphate dehydrogenase (guaB) CSB-YP730304SMZ
CSB-EP730304SMZ
CSB-BP730304SMZ
CSB-MP730304SMZ
CSB-EP730304SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L33 3 (rpmG3) CSB-YP730305SMZ
CSB-EP730305SMZ
CSB-BP730305SMZ
CSB-MP730305SMZ
CSB-EP730305SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA mismatch repair protein MutS (mutS), partial CSB-YP730306SMZ
CSB-EP730306SMZ
CSB-BP730306SMZ
CSB-MP730306SMZ
CSB-EP730306SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0297 protein M6_Spy1796 (M6_Spy1796) CSB-YP730307SMZ
CSB-EP730307SMZ
CSB-BP730307SMZ
CSB-MP730307SMZ
CSB-EP730307SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor Ts (tsf) CSB-YP730308SMZ
CSB-EP730308SMZ
CSB-BP730308SMZ
CSB-MP730308SMZ
CSB-EP730308SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative nrdI-like protein (M6_Spy1696) CSB-YP730309SMZ
CSB-EP730309SMZ
CSB-BP730309SMZ
CSB-MP730309SMZ
CSB-EP730309SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tagatose-6-phosphate kinase (lacC) CSB-YP730310SMZ
CSB-EP730310SMZ
CSB-BP730310SMZ
CSB-MP730310SMZ
CSB-EP730310SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable DNA-directed RNA polymerase subunit delta (rpoE) CSB-YP730311SMZ
CSB-EP730311SMZ
CSB-BP730311SMZ
CSB-MP730311SMZ
CSB-EP730311SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein Spy1595 (M6_Spy1595) CSB-YP730312SMZ
CSB-EP730312SMZ
CSB-BP730312SMZ
CSB-MP730312SMZ
CSB-EP730312SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant N utilization substance protein B homolog (nusB) CSB-YP730313SMZ
CSB-EP730313SMZ
CSB-BP730313SMZ
CSB-MP730313SMZ
CSB-EP730313SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein grpE (grpE) CSB-YP730314SMZ
CSB-EP730314SMZ
CSB-BP730314SMZ
CSB-MP730314SMZ
CSB-EP730314SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant PTS system mannose-specific EIIAB component (manX) CSB-YP730315SMZ
CSB-EP730315SMZ
CSB-BP730315SMZ
CSB-MP730315SMZ
CSB-EP730315SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP730316SMZ
CSB-EP730316SMZ
CSB-BP730316SMZ
CSB-MP730316SMZ
CSB-EP730316SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable transaldolase (tal) CSB-YP730317SMZ
CSB-EP730317SMZ
CSB-BP730317SMZ
CSB-MP730317SMZ
CSB-EP730317SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant NH (3)-dependent NAD (+) synthetase (nadE) CSB-YP730318SMZ
CSB-EP730318SMZ
CSB-BP730318SMZ
CSB-MP730318SMZ
CSB-EP730318SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Guanylate kinase (gmk) CSB-YP730319SMZ
CSB-EP730319SMZ
CSB-BP730319SMZ
CSB-MP730319SMZ
CSB-EP730319SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosome-associated factor Y (M6_Spy1371) CSB-YP730320SMZ
CSB-EP730320SMZ
CSB-BP730320SMZ
CSB-MP730320SMZ
CSB-EP730320SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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