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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant 50S ribosomal protein L4 (rplD) CSB-YP731303BUI
CSB-EP731303BUI
CSB-BP731303BUI
CSB-MP731303BUI
CSB-EP731303BUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor Tu (tuf) CSB-YP731304BUI
CSB-EP731304BUI
CSB-BP731304BUI
CSB-MP731304BUI
CSB-EP731304BUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Adenylate kinase (adk) CSB-YP731305BUI
CSB-EP731305BUI
CSB-BP731305BUI
CSB-MP731305BUI
CSB-EP731305BUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L33 (rpmG) CSB-YP731306BUI
CSB-EP731306BUI
CSB-BP731306BUI
CSB-MP731306BUI
CSB-EP731306BUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S12 (rpsL) CSB-YP731307BUI
CSB-EP731307BUI
CSB-BP731307BUI
CSB-MP731307BUI
CSB-EP731307BUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant S-ribosylhomocysteine lyase (luxS) CSB-YP731308BUI
CSB-EP731308BUI
CSB-BP731308BUI
CSB-MP731308BUI
CSB-EP731308BUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylglyoxal synthase (mgsA) CSB-YP731309BUI
CSB-EP731309BUI
CSB-BP731309BUI
CSB-MP731309BUI
CSB-EP731309BUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP731310BUI
CSB-EP731310BUI
CSB-BP731310BUI
CSB-MP731310BUI
CSB-EP731310BUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cydia pomonella granulosis virus Occlusion-derived virus envelope protein E56 (odv-e56), partial CSB-YP731311EQA1
CSB-EP731311EQA1
CSB-BP731311EQA1
CSB-MP731311EQA1
CSB-EP731311EQA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Colorado tick fever virus Uncharacterized protein VP12, partial CSB-YP731312DVJ1
CSB-EP731312DVJ1
CSB-BP731312DVJ1
CSB-MP731312DVJ1
CSB-EP731312DVJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S21 (rpsU) CSB-YP731313BUI
CSB-EP731313BUI
CSB-BP731313BUI
CSB-MP731313BUI
CSB-EP731313BUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA mismatch repair protein mutL (mutL), partial CSB-YP731314BUI
CSB-EP731314BUI
CSB-BP731314BUI
CSB-MP731314BUI
CSB-EP731314BUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Histidine--tRNA ligase (hisS) CSB-YP731315BUI
CSB-EP731315BUI
CSB-BP731315BUI
CSB-MP731315BUI
CSB-EP731315BUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S18 (rpsR) CSB-YP731316BUI
CSB-EP731316BUI
CSB-BP731316BUI
CSB-MP731316BUI
CSB-EP731316BUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable rRNA maturation factor (BG0059) CSB-YP731317BUI
CSB-EP731317BUI
CSB-BP731317BUI
CSB-MP731317BUI
CSB-EP731317BUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant SsrA-binding protein (smpB) CSB-YP731318BUI
CSB-EP731318BUI
CSB-BP731318BUI
CSB-MP731318BUI
CSB-EP731318BUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP731319BUI
CSB-EP731319BUI
CSB-BP731319BUI
CSB-MP731319BUI
CSB-EP731319BUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase subunit b (atpF), partial CSB-YP731320YAH1
CSB-EP731320YAH1
CSB-BP731320YAH1
CSB-MP731320YAH1
CSB-EP731320YAH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA modification GTPase MnmE (mnmE) CSB-YP731321YAH
CSB-EP731321YAH
CSB-BP731321YAH
CSB-MP731321YAH
CSB-EP731321YAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant L-seryl-tRNA (Sec) selenium transferase (selA) CSB-YP731322YAH
CSB-EP731322YAH
CSB-BP731322YAH
CSB-MP731322YAH
CSB-EP731322YAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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