Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Methanocaldococcus jannaschii Putative antitoxin MJECL31 (MJECL31) CSB-YP730601MRU
CSB-EP730601MRU
CSB-BP730601MRU
CSB-MP730601MRU
CSB-EP730601MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Uncharacterized MCM-type protein MJECL13 (MJECL13), partial CSB-YP730602MRU
CSB-EP730602MRU
CSB-BP730602MRU
CSB-MP730602MRU
CSB-EP730602MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJECL27 (MJECL27) CSB-YP730603MRU
CSB-EP730603MRU
CSB-BP730603MRU
CSB-MP730603MRU
CSB-EP730603MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Putative type I restriction enzyme MjaXP M protein (MJECL42), partial CSB-YP730604MRU
CSB-EP730604MRU
CSB-BP730604MRU
CSB-MP730604MRU
CSB-EP730604MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma hyopneumoniae Protein RecA (recA) CSB-YP730605MWK
CSB-EP730605MWK
CSB-BP730605MWK
CSB-MP730605MWK
CSB-EP730605MWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylococcus capsulatus tRNA modification GTPase MnmE (mnmE) CSB-YP730606MFM
CSB-EP730606MFM
CSB-BP730606MFM
CSB-MP730606MFM
CSB-EP730606MFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylococcus capsulatus Ribonuclease PH (rph) CSB-YP730607MFM
CSB-EP730607MFM
CSB-BP730607MFM
CSB-MP730607MFM
CSB-EP730607MFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylococcus capsulatus Thiamine-phosphate synthase (thiE) CSB-YP730608MFM
CSB-EP730608MFM
CSB-BP730608MFM
CSB-MP730608MFM
CSB-EP730608MFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJECS07 (MJECS07) CSB-YP730609MRU
CSB-EP730609MRU
CSB-BP730609MRU
CSB-MP730609MRU
CSB-EP730609MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJECS09 (MJECS09) CSB-YP730610MRU
CSB-EP730610MRU
CSB-BP730610MRU
CSB-MP730610MRU
CSB-EP730610MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Probable aspartate aminotransferase 1 (MJ0001) CSB-YP730611MRU
CSB-EP730611MRU
CSB-BP730611MRU
CSB-MP730611MRU
CSB-EP730611MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Putative transposase MJ0012 (MJ0012) CSB-YP730612MRU
CSB-EP730612MRU
CSB-BP730612MRU
CSB-MP730612MRU
CSB-EP730612MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii RNA 3'-terminal phosphate cyclase (rtcA) CSB-YP730613MRU
CSB-EP730613MRU
CSB-BP730613MRU
CSB-MP730613MRU
CSB-EP730613MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ0042 (MJ0042) CSB-YP730614MRU
CSB-EP730614MRU
CSB-BP730614MRU
CSB-MP730614MRU
CSB-EP730614MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Putative methyltransferase MJ0046 (MJ0046) CSB-YP730615MRU
CSB-EP730615MRU
CSB-BP730615MRU
CSB-MP730615MRU
CSB-EP730615MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Peptidyl-tRNA hydrolase (pth) CSB-YP730616MRU
CSB-EP730616MRU
CSB-BP730616MRU
CSB-MP730616MRU
CSB-EP730616MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ0067 (MJ0067), partial CSB-YP730617MRU1
CSB-EP730617MRU1
CSB-BP730617MRU1
CSB-MP730617MRU1
CSB-EP730617MRU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Putative toxin RelE1 (relE1) CSB-YP730618MRU
CSB-EP730618MRU
CSB-BP730618MRU
CSB-MP730618MRU
CSB-EP730618MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ0076 (MJ0076) CSB-YP730619MRU
CSB-EP730619MRU
CSB-BP730619MRU
CSB-MP730619MRU
CSB-EP730619MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Methyl-coenzyme M reductase II subunit gamma (mrtG) CSB-YP730620MRU
CSB-EP730620MRU
CSB-BP730620MRU
CSB-MP730620MRU
CSB-EP730620MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>