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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rickettsia typhi UDP-glucose 6-dehydrogenase (udg) CSB-YP731604RNE
CSB-EP731604RNE
CSB-BP731604RNE
CSB-MP731604RNE
CSB-EP731604RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi Protein RecA (recA) CSB-YP731605RNE
CSB-EP731605RNE
CSB-BP731605RNE
CSB-MP731605RNE
CSB-EP731605RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi Uncharacterized hydrolase RT0729 (RT0729) CSB-YP731606RNE
CSB-EP731606RNE
CSB-BP731606RNE
CSB-MP731606RNE
CSB-EP731606RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi Ribonuclease H (rnhA) CSB-YP731607RNE
CSB-EP731607RNE
CSB-BP731607RNE
CSB-MP731607RNE
CSB-EP731607RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi Uncharacterized protein RT0683 (RT0683), partial CSB-YP731608RNE1
CSB-EP731608RNE1
CSB-BP731608RNE1
CSB-MP731608RNE1
CSB-EP731608RNE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP731609RNE
CSB-EP731609RNE
CSB-BP731609RNE
CSB-MP731609RNE
CSB-EP731609RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi 50S ribosomal protein L23 (rplW) CSB-YP731610RNE
CSB-EP731610RNE
CSB-BP731610RNE
CSB-MP731610RNE
CSB-EP731610RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi 50S ribosomal protein L14 (rplN) CSB-YP731611RNE
CSB-EP731611RNE
CSB-BP731611RNE
CSB-MP731611RNE
CSB-EP731611RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi 50S ribosomal protein L30 (rpmD) CSB-YP731612RNE
CSB-EP731612RNE
CSB-BP731612RNE
CSB-MP731612RNE
CSB-EP731612RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi 30S ribosomal protein S20 (rpsT) CSB-YP731613RNE
CSB-EP731613RNE
CSB-BP731613RNE
CSB-MP731613RNE
CSB-EP731613RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi 50S ribosomal protein L34 (rpmH) CSB-YP731614RNE
CSB-EP731614RNE
CSB-BP731614RNE
CSB-MP731614RNE
CSB-EP731614RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE) CSB-YP731615RNE
CSB-EP731615RNE
CSB-BP731615RNE
CSB-MP731615RNE
CSB-EP731615RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP731616RNE
CSB-EP731616RNE
CSB-BP731616RNE
CSB-MP731616RNE
CSB-EP731616RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi Putative fatty acid oxidation complex trifunctional enzyme (RT0547), partial CSB-YP731617RNE
CSB-EP731617RNE
CSB-BP731617RNE
CSB-MP731617RNE
CSB-EP731617RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi Primosomal protein N' (priA), partial CSB-YP731618RNE
CSB-EP731618RNE
CSB-BP731618RNE
CSB-MP731618RNE
CSB-EP731618RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi 30S ribosomal protein S1 (rpsA), partial CSB-YP731619RNE
CSB-EP731619RNE
CSB-BP731619RNE
CSB-MP731619RNE
CSB-EP731619RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi Pyruvate, phosphate dikinase (ppdK), partial CSB-YP731620RNE
CSB-EP731620RNE
CSB-BP731620RNE
CSB-MP731620RNE
CSB-EP731620RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi Isopentenyl-diphosphate delta-isomerase (fni) CSB-YP731621RNE
CSB-EP731621RNE
CSB-BP731621RNE
CSB-MP731621RNE
CSB-EP731621RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP731622RNE
CSB-EP731622RNE
CSB-BP731622RNE
CSB-MP731622RNE
CSB-EP731622RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi Uncharacterized lipoprotein RT0399 (RT0399) CSB-YP731623RNE
CSB-EP731623RNE
CSB-BP731623RNE
CSB-MP731623RNE
CSB-EP731623RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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