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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant African swine fever virus Putative DNA-directed RNA polymerase subunit 5 homolog (Mal-116) CSB-YP731143AYE
CSB-EP731143AYE
CSB-BP731143AYE
CSB-MP731143AYE
CSB-EP731143AYE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Uncharacterized protein H171R (Mal-122) CSB-YP731144AYE
CSB-EP731144AYE
CSB-BP731144AYE
CSB-MP731144AYE
CSB-EP731144AYE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Endoglucanase 17 (GLU13) CSB-YP731145OFG
CSB-EP731145OFG
CSB-BP731145OFG
CSB-MP731145OFG
CSB-EP731145OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Putative germin-like protein 9-2 (Os09g0568600, LOC_Os09g39520) CSB-YP731146OFG
CSB-EP731146OFG
CSB-BP731146OFG
CSB-MP731146OFG
CSB-EP731146OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orgyia pseudotsugata multicapsid polyhedrosis virus Uncharacterized 15.4 kDa protein (ORF73) CSB-YP731147OEU
CSB-EP731147OEU
CSB-BP731147OEU
CSB-MP731147OEU
CSB-EP731147OEU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Banana bunchy top virus Capsid protein (DNA-S) CSB-YP731148BEH
CSB-EP731148BEH
CSB-BP731148BEH
CSB-MP731148BEH
CSB-EP731148BEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Solanesyl-diphosphate synthase 1, mitochondrial (SPS1) CSB-YP731149OFG
CSB-EP731149OFG
CSB-BP731149OFG
CSB-MP731149OFG
CSB-EP731149OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine ephemeral fever virus Protein beta (beta) CSB-YP731150BJQ
CSB-EP731150BJQ
CSB-BP731150BJQ
CSB-MP731150BJQ
CSB-EP731150BJQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus C Outer capsid protein VP4, partial CSB-YP731151RHG
CSB-EP731151RHG
CSB-BP731151RHG
CSB-MP731151RHG
CSB-EP731151RHG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beet necrotic yellow vein virus 31 kDa protein (p31) CSB-YP731152BSO
CSB-EP731152BSO
CSB-BP731152BSO
CSB-MP731152BSO
CSB-EP731152BSO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beet necrotic yellow vein virus Probable suppressor of RNA silencing (ORF7) CSB-YP731153BSO
CSB-EP731153BSO
CSB-BP731153BSO
CSB-MP731153BSO
CSB-EP731153BSO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Zinc finger CCCH domain-containing protein 7 (Os01g0572100, LOC_Os01g39100), partial CSB-YP731154OFG
CSB-EP731154OFG
CSB-BP731154OFG
CSB-MP731154OFG
CSB-EP731154OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Phosphate transporter PHO1-1 (PHO1-1), partial CSB-YP731155OFG1
CSB-EP731155OFG1
CSB-BP731155OFG1
CSB-MP731155OFG1
CSB-EP731155OFG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxsackievirus A16 Genome polyprotein CSB-YP731156CGAV
CSB-EP731156CGAV
CSB-BP731156CGAV
CSB-MP731156CGAV
CSB-EP731156CGAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Canine coronavirus Non-structural protein 3x (3x) CSB-YP731157CCO
CSB-EP731157CCO
CSB-BP731157CCO
CSB-MP731157CCO
CSB-EP731157CCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dog G2/mitotic-specific cyclin-B3 (CCNB3), partial CSB-YP731158DO
CSB-EP731158DO
CSB-BP731158DO
CSB-MP731158DO
CSB-EP731158DO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dog Alpha- (1,3)-fucosyltransferase (FUT9), partial CSB-YP731159DO
CSB-EP731159DO
CSB-BP731159DO
CSB-MP731159DO
CSB-EP731159DO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listonella damsela Hemin import ATP-binding protein HmuV (hmuV) CSB-YP731160LGY
CSB-EP731160LGY
CSB-BP731160LGY
CSB-MP731160LGY
CSB-EP731160LGY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus licheniformis 50S ribosomal protein L9 (rplI) CSB-YP731161BQU
CSB-EP731161BQU
CSB-BP731161BQU
CSB-MP731161BQU
CSB-EP731161BQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus licheniformis Methylmalonate semialdehyde dehydrogenase [acylating] 2 (iolA2) CSB-YP731162BQU
CSB-EP731162BQU
CSB-BP731162BQU
CSB-MP731162BQU
CSB-EP731162BQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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