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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus licheniformis UPF0133 protein BLi00029/BL02358 (BLi00029, BL02358) CSB-YP731223BQU
CSB-EP731223BQU
CSB-BP731223BQU
CSB-MP731223BQU
CSB-EP731223BQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens tRNA (uracil (54)-C (5))-methyltransferase (trmA) CSB-YP731224MAAB
CSB-EP731224MAAB
CSB-BP731224MAAB
CSB-MP731224MAAB
CSB-EP731224MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens ATP synthase subunit beta (atpD) CSB-YP731225MAAB
CSB-EP731225MAAB
CSB-BP731225MAAB
CSB-MP731225MAAB
CSB-EP731225MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Multifunctional CCA protein (cca) CSB-YP731226MAAB
CSB-EP731226MAAB
CSB-BP731226MAAB
CSB-MP731226MAAB
CSB-EP731226MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens 33 kDa chaperonin (hslO) CSB-YP731227MAAB
CSB-EP731227MAAB
CSB-BP731227MAAB
CSB-MP731227MAAB
CSB-EP731227MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens UPF0350 protein MS2227 (MS2227) CSB-YP731228MAAB
CSB-EP731228MAAB
CSB-BP731228MAAB
CSB-MP731228MAAB
CSB-EP731228MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Peptide deformylase (def) CSB-YP731229MAAB
CSB-EP731229MAAB
CSB-BP731229MAAB
CSB-MP731229MAAB
CSB-EP731229MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Thiosulfate sulfurtransferase glpE (glpE) CSB-YP731230MAAB
CSB-EP731230MAAB
CSB-BP731230MAAB
CSB-MP731230MAAB
CSB-EP731230MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Maltoporin (lamB) CSB-YP731231MAAB
CSB-EP731231MAAB
CSB-BP731231MAAB
CSB-MP731231MAAB
CSB-EP731231MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens 50S ribosomal protein L4 (rplD) CSB-YP731232MAAB
CSB-EP731232MAAB
CSB-BP731232MAAB
CSB-MP731232MAAB
CSB-EP731232MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens 50S ribosomal protein L30 (rpmD) CSB-YP731233MAAB
CSB-EP731233MAAB
CSB-BP731233MAAB
CSB-MP731233MAAB
CSB-EP731233MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Anaerobic glycerol-3-phosphate dehydrogenase subunit B (glpB) CSB-YP731234MAAB
CSB-EP731234MAAB
CSB-BP731234MAAB
CSB-MP731234MAAB
CSB-EP731234MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP731235MAAB
CSB-EP731235MAAB
CSB-BP731235MAAB
CSB-MP731235MAAB
CSB-EP731235MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Histidine--tRNA ligase (hisS) CSB-YP731236MAAB
CSB-EP731236MAAB
CSB-BP731236MAAB
CSB-MP731236MAAB
CSB-EP731236MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Phosphate acyltransferase (plsX) CSB-YP731237MAAB
CSB-EP731237MAAB
CSB-BP731237MAAB
CSB-MP731237MAAB
CSB-EP731237MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Bifunctional protein FolD (folD) CSB-YP731238MAAB
CSB-EP731238MAAB
CSB-BP731238MAAB
CSB-MP731238MAAB
CSB-EP731238MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Membrane-bound lytic murein transglycosylase F (mltF) CSB-YP731239MAAB
CSB-EP731239MAAB
CSB-BP731239MAAB
CSB-MP731239MAAB
CSB-EP731239MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Diaminopimelate epimerase (dapF) CSB-YP731240MAAB
CSB-EP731240MAAB
CSB-BP731240MAAB
CSB-MP731240MAAB
CSB-EP731240MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Chaperone protein hscA homolog (hscA), partial CSB-YP731241MAAB
CSB-EP731241MAAB
CSB-BP731241MAAB
CSB-MP731241MAAB
CSB-EP731241MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Chaperone protein htpG (htpG), partial CSB-YP731242MAAB
CSB-EP731242MAAB
CSB-BP731242MAAB
CSB-MP731242MAAB
CSB-EP731242MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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