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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pinus sibirica Maturase K (matK), partial CSB-YP731864PGAJ
CSB-EP731864PGAJ
CSB-BP731864PGAJ
CSB-MP731864PGAJ
CSB-EP731864PGAJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human UHRF1-binding protein 1 (UHRF1BP1), partial CSB-YP731865HU
CSB-EP731865HU
CSB-BP731865HU
CSB-MP731865HU
CSB-EP731865HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Eustoma exaltatum subsp. russellianum Chalcone--flavonone isomerase (CHI) CSB-YP731866EPS
CSB-EP731866EPS
CSB-BP731866EPS
CSB-MP731866EPS
CSB-EP731866EPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 2-dehydro-3-deoxy-6-phosphogalactonate aldolase (dgoA) CSB-YP731867ENV
CSB-EP731867ENV
CSB-BP731867ENV
CSB-MP731867ENV
CSB-EP731867ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Iron sulfur cluster assembly protein 1, mitochondrial (ISU1), partial CSB-YP731868DIS
CSB-EP731868DIS
CSB-BP731868DIS
CSB-MP731868DIS
CSB-EP731868DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Putative DNA helicase INO80 (INO80), partial CSB-YP731869DIS
CSB-EP731869DIS
CSB-BP731869DIS
CSB-MP731869DIS
CSB-EP731869DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Autophagy protein 16 (ATG16) CSB-YP731870DIS
CSB-EP731870DIS
CSB-BP731870DIS
CSB-MP731870DIS
CSB-EP731870DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Autophagy-related protein 23 (ATG23), partial CSB-YP731871DIS
CSB-EP731871DIS
CSB-BP731871DIS
CSB-MP731871DIS
CSB-EP731871DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Palmitoyltransferase ERF2 (ERF2), partial CSB-YP731872DIS1
CSB-EP731872DIS1
CSB-BP731872DIS1
CSB-MP731872DIS1
CSB-EP731872DIS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Assembly factor CBP4 (CBP4), partial CSB-YP731873DIS1
CSB-EP731873DIS1
CSB-BP731873DIS1
CSB-MP731873DIS1
CSB-EP731873DIS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Polyadenylate-binding protein, cytoplasmic and nuclear (PAB1), partial CSB-YP731874DIS
CSB-EP731874DIS
CSB-BP731874DIS
CSB-MP731874DIS
CSB-EP731874DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Protein FYV4, mitochondrial (FYV4) CSB-YP731875DIS
CSB-EP731875DIS
CSB-BP731875DIS
CSB-MP731875DIS
CSB-EP731875DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii RNA exonuclease 4 (REX4) CSB-YP731876DIS
CSB-EP731876DIS
CSB-BP731876DIS
CSB-MP731876DIS
CSB-EP731876DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Probable cytosolic iron-sulfur protein assembly protein 1 (CIA1) CSB-YP731877DIS
CSB-EP731877DIS
CSB-BP731877DIS
CSB-MP731877DIS
CSB-EP731877DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Acetate non-utilizing protein 9, mitochondrial (ACN9) CSB-YP731878DIS
CSB-EP731878DIS
CSB-BP731878DIS
CSB-MP731878DIS
CSB-EP731878DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Pre-rRNA-processing protein PNO1 (PNO1) CSB-YP731879DIS
CSB-EP731879DIS
CSB-BP731879DIS
CSB-MP731879DIS
CSB-EP731879DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Carbamoyl-phosphate synthase arginine-specific small chain (CPA1) CSB-YP731880DIS
CSB-EP731880DIS
CSB-BP731880DIS
CSB-MP731880DIS
CSB-EP731880DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii ATP-dependent RNA helicase DHH1 (DHH1), partial CSB-YP731881DIS
CSB-EP731881DIS
CSB-BP731881DIS
CSB-MP731881DIS
CSB-EP731881DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Mitochondrial inner membrane protease ATP23 (ATP23) CSB-YP731882DIS
CSB-EP731882DIS
CSB-BP731882DIS
CSB-MP731882DIS
CSB-EP731882DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii ATP-dependent RNA helicase DBP7 (DBP7), partial CSB-YP731883DIS
CSB-EP731883DIS
CSB-BP731883DIS
CSB-MP731883DIS
CSB-EP731883DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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