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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Desulfotalea psychrophila 50S ribosomal protein L3 (rplC) CSB-YP731784DAAA
CSB-EP731784DAAA
CSB-BP731784DAAA
CSB-MP731784DAAA
CSB-EP731784DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila 50S ribosomal protein L10 (rplJ) CSB-YP731785DAAA
CSB-EP731785DAAA
CSB-BP731785DAAA
CSB-MP731785DAAA
CSB-EP731785DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Uncharacterized transporter DP0976 (DP0976), partial CSB-YP731786DAAA
CSB-EP731786DAAA
CSB-BP731786DAAA
CSB-MP731786DAAA
CSB-EP731786DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP731787DAAA1
CSB-EP731787DAAA1
CSB-BP731787DAAA1
CSB-MP731787DAAA1
CSB-EP731787DAAA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila ATP synthase subunit b (atpF), partial CSB-YP731788DAAA1
CSB-EP731788DAAA1
CSB-BP731788DAAA1
CSB-MP731788DAAA1
CSB-EP731788DAAA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB) CSB-YP731789DAAA
CSB-EP731789DAAA
CSB-BP731789DAAA
CSB-MP731789DAAA
CSB-EP731789DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C (gatC) CSB-YP731790DAAA
CSB-EP731790DAAA
CSB-BP731790DAAA
CSB-MP731790DAAA
CSB-EP731790DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Acetate kinase (ackA) CSB-YP731791DAAA
CSB-EP731791DAAA
CSB-BP731791DAAA
CSB-MP731791DAAA
CSB-EP731791DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Argininosuccinate synthase (argG) CSB-YP731792DAAA
CSB-EP731792DAAA
CSB-BP731792DAAA
CSB-MP731792DAAA
CSB-EP731792DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Octanoyltransferase (lipB) CSB-YP731793DAAA
CSB-EP731793DAAA
CSB-BP731793DAAA
CSB-MP731793DAAA
CSB-EP731793DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Putative cobalt-precorrin-6A synthase [deacetylating] (cbiD) CSB-YP731794DAAA
CSB-EP731794DAAA
CSB-BP731794DAAA
CSB-MP731794DAAA
CSB-EP731794DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP731795DAAA
CSB-EP731795DAAA
CSB-BP731795DAAA
CSB-MP731795DAAA
CSB-EP731795DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Phosphoglycerate kinase (pgk) CSB-YP731796DAAA
CSB-EP731796DAAA
CSB-BP731796DAAA
CSB-MP731796DAAA
CSB-EP731796DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Homeobox-leucine zipper protein HOX32 (HOX32), partial CSB-YP731797OFG
CSB-EP731797OFG
CSB-BP731797OFG
CSB-MP731797OFG
CSB-EP731797OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Ninja-family protein Os05g0558800 (Os05g0558800, LOC_Os05g48500) CSB-YP731798OFG
CSB-EP731798OFG
CSB-BP731798OFG
CSB-MP731798OFG
CSB-EP731798OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Peptide methionine sulfoxide reductase B3, chloroplastic (MSRB3) CSB-YP731799OFG
CSB-EP731799OFG
CSB-BP731799OFG
CSB-MP731799OFG
CSB-EP731799OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable metal-nicotianamine transporter YSL3 (YSL3), partial CSB-YP731800OFG
CSB-EP731800OFG
CSB-BP731800OFG
CSB-MP731800OFG
CSB-EP731800OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Carboxylesterase 5A (Ces5a) CSB-YP731801MO
CSB-EP731801MO
CSB-BP731801MO
CSB-MP731801MO
CSB-EP731801MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (CG32068) CSB-YP731802DLU
CSB-EP731802DLU
CSB-BP731802DLU
CSB-MP731802DLU
CSB-EP731802DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Sulfhydryl oxidase 2 (qsox2), partial CSB-YP731803XBE1
CSB-EP731803XBE1
CSB-BP731803XBE1
CSB-MP731803XBE1
CSB-EP731803XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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