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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Human Ribonuclease H2 subunit B (RNASEH2B) CSB-YP732934HU
CSB-EP732934HU
CSB-BP732934HU
CSB-MP732934HU
CSB-EP732934HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Roquin-1 (RC3H1), partial CSB-YP732935HU
CSB-EP732935HU
CSB-BP732935HU
CSB-MP732935HU
CSB-EP732935HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Tau-tubulin kinase 1 (TTBK1), partial CSB-YP732936HU
CSB-EP732936HU
CSB-BP732936HU
CSB-MP732936HU
CSB-EP732936HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein DDI1 homolog 2 (DDI2) CSB-YP732937HU
CSB-EP732937HU
CSB-BP732937HU
CSB-MP732937HU
CSB-EP732937HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative uncharacterized protein C6orf164 (C6orf164) CSB-YP732938HU
CSB-EP732938HU
CSB-BP732938HU
CSB-MP732938HU
CSB-EP732938HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Signal-regulatory protein beta-1 isoform 3 (SIRPB1), partial CSB-YP732939HU
CSB-EP732939HU
CSB-BP732939HU
CSB-MP732939HU
CSB-EP732939HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Sterile alpha motif domain-containing protein 5 (SAMD5) CSB-YP732940HU
CSB-EP732940HU
CSB-BP732940HU
CSB-MP732940HU
CSB-EP732940HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Brefeldin A-inhibited guanine nucleotide-exchange protein 3 (ARFGEF3), partial CSB-YP732941HU
CSB-EP732941HU
CSB-BP732941HU
CSB-MP732941HU
CSB-EP732941HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dog Zinc finger and BTB domain-containing protein 22 (ZBTB22), partial CSB-YP732942DO
CSB-EP732942DO
CSB-BP732942DO
CSB-MP732942DO
CSB-EP732942DO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dog Beta-1,3-galactosyltransferase 4 (B3GALT4), partial CSB-YP732943DO
CSB-EP732943DO
CSB-BP732943DO
CSB-MP732943DO
CSB-EP732943DO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dog Zinc transporter SLC39A7 (SLC39A7), partial CSB-YP732944DO1
CSB-EP732944DO1
CSB-BP732944DO1
CSB-MP732944DO1
CSB-EP732944DO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Histone acetyltransferase KAT6A (Kat6a), partial CSB-YP732945RA
CSB-EP732945RA
CSB-BP732945RA
CSB-MP732945RA
CSB-EP732945RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca mulatta Lymphotoxin-beta (LTB), partial CSB-YP732946MOW
CSB-EP732946MOW
CSB-BP732946MOW
CSB-MP732946MOW
CSB-EP732946MOW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca mulatta Serine/threonine-protein phosphatase 1 regulatory subunit 10 (PPP1R10), partial CSB-YP732947MOW
CSB-EP732947MOW
CSB-BP732947MOW
CSB-MP732947MOW
CSB-EP732947MOW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca mulatta Phostensin (PPP1R18), partial CSB-YP732948MOW
CSB-EP732948MOW
CSB-BP732948MOW
CSB-MP732948MOW
CSB-EP732948MOW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anopheles gambiae Glucosamine-6-phosphate isomerase (Gnpda1) CSB-YP732949BZL
CSB-EP732949BZL
CSB-BP732949BZL
CSB-MP732949BZL
CSB-EP732949BZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anopheles gambiae Probable 39S ribosomal protein L23, mitochondrial (mRpL23) CSB-YP732950BZL
CSB-EP732950BZL
CSB-BP732950BZL
CSB-MP732950BZL
CSB-EP732950BZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anopheles gambiae Structure-specific endonuclease subunit SLX1 homolog (AGAP002259) CSB-YP732951BZL
CSB-EP732951BZL
CSB-BP732951BZL
CSB-MP732951BZL
CSB-EP732951BZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio UPF0480 protein C15orf24 homolog (si:ch211-150c22.3), partial CSB-YP732952DIL
CSB-EP732952DIL
CSB-BP732952DIL
CSB-MP732952DIL
CSB-EP732952DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ankyrin repeat domain-containing protein 45 (ANKRD45) CSB-YP732953HU
CSB-EP732953HU
CSB-BP732953HU
CSB-MP732953HU
CSB-EP732953HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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