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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rhomboid-like protease 1 (ROM1), partial CSB-YP734740TOV1
CSB-EP734740TOV1
CSB-BP734740TOV1
CSB-MP734740TOV1
CSB-EP734740TOV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dog Transient receptor potential cation channel subfamily V member 1 (TRPV1), partial CSB-YP734741DO
CSB-EP734741DO
CSB-BP734741DO
CSB-MP734741DO
CSB-EP734741DO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Eriocheir sinensis reovirus RNA-directed RNA polymerase, partial CSB-YP734742ECAJ
CSB-EP734742ECAJ
CSB-BP734742ECAJ
CSB-MP734742ECAJ
CSB-EP734742ECAJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ziphius cavirostris NADH-ubiquinone oxidoreductase chain 4L (MT-ND4L), partial CSB-YP734743ZAI1
CSB-EP734743ZAI1
CSB-BP734743ZAI1
CSB-MP734743ZAI1
CSB-EP734743ZAI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo pygmaeus Cytochrome b-c1 complex subunit Rieske, mitochondrial (UQCRFS1), partial CSB-YP734744EXP1
CSB-EP734744EXP1
CSB-BP734744EXP1
CSB-MP734744EXP1
CSB-EP734744EXP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ophiophagus hannah Cytotoxin 27 CSB-YP734745OEL
CSB-EP734745OEL
CSB-BP734745OEL
CSB-MP734745OEL
CSB-EP734745OEL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trichophyton rubrum Subtilisin-like protease 5 (SUB5) CSB-YP734746TQA
CSB-EP734746TQA
CSB-BP734746TQA
CSB-MP734746TQA
CSB-EP734746TQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lagenorhynchus acutus Cytochrome P450 19A1 (CYP19A1), partial CSB-YP734747LCAQ
CSB-EP734747LCAQ
CSB-BP734747LCAQ
CSB-MP734747LCAQ
CSB-EP734747LCAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Sucrose transport protein SUT5 (SUT5), partial CSB-YP734748OFG
CSB-EP734748OFG
CSB-BP734748OFG
CSB-MP734748OFG
CSB-EP734748OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Laccase-14 (LAC14), partial CSB-YP734749OFG
CSB-EP734749OFG
CSB-BP734749OFG
CSB-MP734749OFG
CSB-EP734749OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Ubiquitin-like protein ATG12 (ATG12) CSB-YP734750OFG
CSB-EP734750OFG
CSB-BP734750OFG
CSB-MP734750OFG
CSB-EP734750OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Heat shock protein 81-2 (HSP81-2), partial CSB-YP734751OFG
CSB-EP734751OFG
CSB-BP734751OFG
CSB-MP734751OFG
CSB-EP734751OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Cytochrome b6-f complex iron-sulfur subunit, chloroplastic (petC), partial CSB-YP734752OFG1
CSB-EP734752OFG1
CSB-BP734752OFG1
CSB-MP734752OFG1
CSB-EP734752OFG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Ferrochelatase-1, chloroplastic (Os09g0297000, LOC_Os09g12560) CSB-YP734753OFG
CSB-EP734753OFG
CSB-BP734753OFG
CSB-MP734753OFG
CSB-EP734753OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Tubby-like F-box protein 12 (TULP12) CSB-YP734754OFG
CSB-EP734754OFG
CSB-BP734754OFG
CSB-MP734754OFG
CSB-EP734754OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Protein argonaute PNH1 (PHN1), partial CSB-YP734755OFG
CSB-EP734755OFG
CSB-BP734755OFG
CSB-MP734755OFG
CSB-EP734755OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Cytochrome P450 734A4 (CYP734A4), partial CSB-YP734756OFG
CSB-EP734756OFG
CSB-BP734756OFG
CSB-MP734756OFG
CSB-EP734756OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative pleckstrin homology domain-containing family M member 1P (PLEKHM1P) CSB-YP734757HU
CSB-EP734757HU
CSB-BP734757HU
CSB-MP734757HU
CSB-EP734757HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Uncharacterized protein C1orf222 (C1orf222) CSB-YP734758HU
CSB-EP734758HU
CSB-BP734758HU
CSB-MP734758HU
CSB-EP734758HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Sterile alpha motif domain-containing protein 9-like (Samd9l), partial CSB-YP734759MO
CSB-EP734759MO
CSB-BP734759MO
CSB-MP734759MO
CSB-EP734759MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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